An Optimised Direct Lysis Technique for Viral RNA Extraction and Detection of Foodborne Viruses on Fruits and Greens

Blood Direct PCR Kit V2
PD103-01	Vazyme	50 rxn	EUR 169

Blood Direct PCR Kit V2
PD103-02	Vazyme	200 rxn	EUR 354

MyTaq Plant-PCR Kit
BIO-25055	Bioline	250 reactions	Ask for price

MyTaq Plant-PCR Kit
BIO-25056	Bioline	500 reactions	Ask for price

Plant Tissue PCR Kit
20-abx09801220ulSystems	Abbexa	EUR 286.00 EUR 690.00	100 rxns × 20 ul Systems 500 rxns × 20 ul Systems

Plant Advance? PCR Kit
M1144-25	Biovision		EUR 376

Plant Preservative Mixture
PCT01	Plant Cell Technologies	30 ml	EUR 90
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture
PCT02	Plant Cell Technologies	100 ml	EUR 749
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture
PCT03	Plant Cell Technologies	250 ml	EUR 310
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture
PCT04	Plant Cell Technologies	500 ml	EUR 450
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture
PCT05	Plant Cell Technologies	1000 ml	EUR 799
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

PCR Mycoplasma Detection Kit
M034-Kit	TOKU-E	Kit	EUR 266

Mouse Direct PCR Kit (For Genotyping)
B40013	Bimake	200 ractions	EUR 209
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B40015	Bimake	500 ractions	EUR 409
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B45012	Bimake	1ml*5 (500 ractions)	EUR 280
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B45013	Bimake	1ml*20 (2000 ractions)	EUR 865
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

quickAmp? Plant PCR Kit, 1000rxns
A2500-1000RXN	ACTGene		EUR 1890

quickAmp? Plant PCR Kit, 100rxns
A2500-100RXN	ACTGene		EUR 259

amfiSure Direct PCR Lysis Buffer
D0300-010	GenDepot	100ml	EUR 138

AXYPREP MAG PLANT DNA-10 PREP
MAG-PLANT-gDNA-10	CORNING	1/pk	EUR 72
Description: Bioscience Miscellaneous; Bioscience Miscellaneous - Axygen

AXYPREP MAG PLANT DNA-384 PREP
MAG-PLANT-gDNA-M	CORNING	1/pk	EUR 742
Description: Bioscience Miscellaneous; Bioscience Miscellaneous - Axygen

AXYPREP MAG PLANT DNA-96 PREP
MAG-PLANT-gDNA-S	CORNING	1/pk	EUR 415
Description: Bioscience Miscellaneous; Bioscience Miscellaneous - Axygen

Supreme Plant Tissue Culture Grade Agar
PCTA100	Plant Cell Technologies	500g	EUR 120
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Supreme Plant Tissue Culture Grade Agar
PCTA500	Plant Cell Technologies	1000g	EUR 150
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Direct RT-PCR Kit wioRealStart DNA Polymerase
FYT507-100P	Yeastern Biotech	100 Preps	Ask for price

Direct RT-PCR Kit wioRealStart DNA Polymerase
FYT507-50P	Yeastern Biotech	50 Preps	Ask for price

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-48D	Bioingentech	50T	EUR 453

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-96D	Bioingentech	100T	EUR 572

Direct RT-PCR Kit wi RealStart DNA Polymerase
FYT506-100P	Yeastern Biotech	100 Preps	Ask for price

Direct RT-PCR Kit wi RealStart DNA Polymerase
FYT506-50P	Yeastern Biotech	50 Preps;	Ask for price

Allele-In-One Human Blood Direct PCR Kit
ABP-PP-BD02100	Allele Biotech	100 RXNS	Ask for price

Allele-In-One Human Blood Direct PCR Kit
ABP-PP-BD02500	Allele Biotech	500 RXNS	Ask for price

Allele-In-One Mouse Tail Direct PCR Kit
ABP-PP-MT02100	Allele Biotech	100 RXNS	Ask for price

Allele-In-One Mouse Tail Direct PCR Kit
ABP-PP-MT02500	Allele Biotech	500 RXNS	Ask for price

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-48D	Bioingentech	50T	EUR 453

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-96D	Bioingentech	100T	EUR 572

Halobacillus PCR kit
PCR-A635-48D	Bioingentech	50T	EUR 453

Halobacillus PCR kit
PCR-A635-96D	Bioingentech	100T	EUR 572

Enterovirus PCR kit
PCR-H433-48R	Bioingentech	50T	EUR 823

Enterovirus PCR kit
PCR-H433-96R	Bioingentech	100T	EUR 859

Mengovirus PCR kit
PCR-H453-48R	Bioingentech	50T	EUR 823

Mengovirus PCR kit
PCR-H453-96R	Bioingentech	100T	EUR 1113

Astrovirus PCR kit
PCR-H491-48R	Bioingentech	50T	EUR 823

Astrovirus PCR kit
PCR-H491-96R	Bioingentech	100T	EUR 1113

Calicivirus PCR kit
PCR-H493-48R	Bioingentech	50T	EUR 823

Calicivirus PCR kit
PCR-H493-96R	Bioingentech	100T	EUR 1113

Adenovirus PCR kit
PCR-H501-48D	Bioingentech	50T	EUR 453

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miR‑192‑5p upregulation mediates the suppression of curcumin in human NSCLC cell proliferation, migration and invasion by concentrating on c‑Myc and inactivating the Wnt/β‑catenin signaling pathway

Curcumin is a naturally lively phenolic compound extracted from the rhizome of the plant Curcuma longa, which has been demonstrated to function an anticancer drug in various kinds of most cancers, together with non‑small‑cell lung most cancers (NSCLC). Accumulating proof has steered that curcumin might exert epigenetic regulatory results on microRNAs (miRs).

Subsequently, the current examine aimed to research the position of miR‑192‑5p, and the consequences of curcumin, in NSCLC, alongside the underlying mechanisms. Human NSCLC cells, A427 and A549, have been handled with curcumin, and the expression ranges of miR‑192‑5p and c‑Myc have been detected utilizing reverse transcription‑quantitative PCR and western blotting. Mobile proliferation was analyzed utilizing Cell Counting Package‑eight assays and cell viability was decided utilizing a MTT assay.

Moreover, the migratory and invasive skills of cells have been analyzed utilizing Transwell and Matrigel assays, respectively. The binding websites between miR‑192‑5p and c‑Myc have been predicted utilizing TargetScanHuman software program, and confirmed utilizing a twin‑luciferase reporter assay and RNA immunoprecipitation.

Lastly, the Wnt pathway regulator, β‑catenin, and cyclin D1 expression ranges have been decided utilizing western blotting. Curcumin remedy inhibited NSCLC cell proliferation, migration, invasion and viability in a dose‑dependent method, along with selling a dose‑dependent enhance within the expression ranges of miR‑192‑5p and a discount in c‑Myc expression ranges.

Notably, the genetic knockdown of miR‑192‑5p blocked the inhibitory results of curcumin on NSCLC development and as an alternative promoted NSCLC development, which was noticed to be partially reversed by c‑Myc silencing; thus, c‑Myc was steered to be a direct goal gene of miR‑192‑5p as demonstrated by the TargetScanHuman database, twin‑lucierase and RIP assay outcomes.

As well as, the curcumin‑induced decreased expression ranges of β‑catenin, cyclin D1 and c‑Myc have been rescued following the genetic knockdown of miR‑192‑5p. In conclusion, these findings steered that the upregulation of miR‑192‑5p might underlie the inhibitory results of curcumin on NSCLC cells by concentrating on c‑Myc and inactivating the Wnt/β‑catenin signaling pathway.

MULTIPLEX KIT PCR Babesia & Theileria PCR package