Best Viral RNA Plant Direct PCR Kit

Genetici-technologies

An Optimised Direct Lysis Technique for Viral RNA Extraction and Detection of Foodborne Viruses on Fruits and Greens

 

Detection of norovirus (NoV) and hepatitis A virus (HAV) on fruit and veggies utilizing present normal methodologies might be inefficient. Technique optimisation focussing on ease, rapidity and elevated viral RNA restoration is required for environment friendly reverse transcription (RT)-qPCR detection of viruses.
A easy and fast direct lysis technique for RNA extraction was optimised (technique A) to realize elevated viral RNA restoration and minimised RT-qPCR inhibition by growing the amount of lysis buffer and inclusion of pectinase, Plant RNA Isolation Support and OneStep PCR Inhibitor Removing Package.
Technique A and an inside technique structurally similar to the ISO 15216 normal (technique B) have been in contrast for his or her efficiencies to get well viral RNA from the method controls, mengovirus (MC0) and murine norovirus (MNV), spiked in 13 forms of fruits, greens, compound meals or seeds/nuts. All extracts (> 61) have been additionally analysed for RT-qPCR inhibition and for pure contamination of NoV and HAV.
The general imply extraction efficiencies of MC0 and MNV have been 36 ± 31 and 44 ± 38%, respectively, for technique A and 9 ± 16 and 5 ± 11%, respectively, for technique B. Inhibition of RT-qPCR amplification of RNA from NoV genogroup (G)I, NoV GII, and HAV ranged from 5 ± 10 to 13 ± 14% for technique A and 34 ± 36 to 48 ± 40% for technique B.
NoV GII was detected in samples of strawberries and seaweed processed by each strategies. In conclusion, the brand new direct lysis technique confirmed an total higher efficiency in comparison with the modified ISO 15216 normal and needs to be validated for implementation in evaluation of viruses in meals of plant origin.
Key phrases: Dates; Hepatitis A virus; Norovirus; PCR inhibition; Restoration effectivity; Seaweed.

 

Genetici-technologies
Genetici-technologies

Direct Plant PCR Kit
E0960-02 500reactions
EUR 251.79
Description: Enables to perform PCR reactions directly from plants with no prior DNA extraction or purification

FastAmp® Plant Direct PCR Kit
4615 1/EA
EUR 396
Description: Intact Genomics FastAmpTM Plant Direct PCR kit is an easy and robust method to amplify DNA fragments from plant tissues with high specificity and high sensitivity without the need of complicated DNA purification steps. The advanced formulation of this kit also allows fast PCR cycling conditions without compromising PCR sensitivity, specificity and yield. The FastAmpTM Plant Direct PCR kit is an ideal and powerful tool for high-throughput genotyping, DNA amplification, and plant genome analysis.Highlights:Direct PCR- no need to purify DNASpecially engineered Taq DNA polymerase with highest sensitivity and specificityExtremely short PCR protocol timesMaster mix format with premixed gel loading dye to reduce cross-contamination and sample handlingerrors5x magic enhancer for high GC containing DNA amplificationProduct Includes:FastAmp™ plant direct PCR master mix (2x)Dilution bufferControl primer mix (25 µM each)5x magic enhancerNuclease- free water

FastAmp® Plant Direct PCR & Genotyping Solution
4611 20mL
EUR 156
Description: FastAmp® Plant Direct PCR/Genotyping Solution for is designed for rapid cell lysis and DNA separation simultaneously without the need of DNA extraction steps . It contains very low concentration of less toxic chemicals for direct DNA amplification of genomic DNA from various plant tissues. No heat treatment and no termination buffer is is required, saving significant time. FastAmp® Plant Direct PCR/Genotyping Solution has been optimized with FastAmp® Plant Direct PCR kit and FastAmp® Plant Tissue/Seed Genotyping PCR kit.

Direct PCRKits for Plant
FYT303-100P 100 Preps Ask for price

Plant Leaf Direct PCR Kit植物叶片直接PCR试剂盒
4402A 100 Tests
EUR 150

Plant Leaf Direct PCR Kit植物叶片直接PCR试剂盒
4402B 500 Tests
EUR 500

Plant Seed Direct PCR Kit植物种子直接PCR试剂盒
4409A 100 Tests
EUR 500

Plant Seed Direct PCR Kit植物种子直接PCR试剂盒
4409B 500 Tests
EUR 500

Phire Plant Direct PCR Kit (without sampling tools) - 200 x 50 µL rxns
THF-130WH each
EUR 323.73

Phire Plant Direct PCR Master Mix - 500 x 50 µl rxns
THF-160L each
EUR 648.55

Phire Plant Direct PCR Master Mix - 100 x 50 µl rxns
THF-160S each
EUR 165.68

Direct PCR Complete Kit
S700 100 rcs
EUR 159.6

Direct PCR Complete Kit
S700-2 5x100 rcs
EUR 628.8

Direct Blood PCR Kit
E0950-01 100reactions
EUR 69.76
Description: Enables to perform PCR reactions directly from whole blood with no prior DNA extraction or purification

Direct Blood PCR Kit
E0950-02 500reactions
EUR 277.95
Description: Enables to perform PCR reactions directly from whole blood with no prior DNA extraction or purification

Direct Blood PCR Kit
MBPCR171-100R 1 unit
EUR 267.52
Description: Direct Blood PCR Kit

Direct Blood PCR Kit
MBPCR171-10R 1 unit
EUR 71.73
Description: Direct Blood PCR Kit

Blood Direct PCR Kit V2
PD103-01 50 rxn
EUR 51.41

Blood Direct PCR Kit V2
PD103-02 200 rxn
EUR 188.5

Direct Tissue PCR Kit
E0940-01 100reactions
EUR 55.59
Description: Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no priorDNA purification

Direct Tissue PCR Kit
E0940-02 500reactions
EUR 251.79
Description: Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no priorDNA purification

Mouse tissue direct PCR kit
EP033 50T
EUR 125

Mouse tissue direct PCR kit
EP033-200T 200T
EUR 125

Mouse tissue direct PCR kit
EP033-50T 50T
EUR 40

Mouse Tissue Direct PCR Kit
RK20736 100RXN
EUR 149.88

EzWay Mouse Tail Direct PCR Kit
K0568600 1set
EUR 188
Description: https://komabiotech.co.kr/labiskoma/datasheet/K0568600.pdf

Mouse Direct PCR Kit (For Genotyping)
B40013 200 ractions
EUR 250.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B40015 500 ractions
EUR 490.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B45012 1ml*5 (500 ractions)
EUR 336
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B45013 1ml*20 (2000 ractions)
EUR 1038
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Plant Preservative Mixture
PCT02 100 ml
EUR 898.8
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture
PCT03 250 ml
EUR 372
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture
PCT04 500 ml
EUR 540
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture PPM
PCT05 1000 ml
EUR 958.8
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Mouse Tissue Direct PCR Kit (With Dye)
10185ES50 50T
EUR 65

Mouse Tissue Direct PCR Kit (With Dye)
10185ES70 200T
EUR 235

BioLit Animal Tissue Direct PCR Kit (Research)
91210 50 Preps
EUR 222.28
Description: Part E

Supreme Plant Tissue Culture Grade Agar
PCTA100 500g
EUR 144
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Supreme Plant Tissue Culture Grade Agar
PCTA500 1000g
EUR 180
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Allele-In-One Mouse Tail Direct PCR Kit
ABP-PP-MT02100 100 RXNS Ask for price

Allele-In-One Mouse Tail Direct PCR Kit
ABP-PP-MT02500 500 RXNS Ask for price

amfiSure Direct PCR Lysis Buffer
D0300-010 100ml
EUR 165.6

Allele-In-One Human Blood Direct PCR Kit
ABP-PP-BD02100 100 RXNS Ask for price

Allele-In-One Human Blood Direct PCR Kit
ABP-PP-BD02500 500 RXNS Ask for price

Saliva Direct PCR Kit唾液直接PCR试剂盒
4405A 100 Tests
EUR 500

Saliva Direct PCR Kit唾液直接PCR试剂盒
4405B 500 Tests
EUR 500

Mousetail Direct PCR Kit鼠尾直接PCR试剂盒
4404A 100 Tests
EUR 500

 

miR‑192‑5p upregulation mediates the suppression of curcumin in human NSCLC cell proliferation, migration and invasion by concentrating on c‑Myc and inactivating the Wnt/β‑catenin signaling pathway

 

Curcumin is a naturally lively phenolic compound extracted from the rhizome of the plant Curcuma longa, which has been demonstrated to function an anticancer drug in various kinds of most cancers, together with non‑small‑cell lung most cancers (NSCLC). Accumulating proof has steered that curcumin might exert epigenetic regulatory results on microRNAs (miRs).

 

Subsequently, the current examine aimed to research the position of miR‑192‑5p, and the consequences of curcumin, in NSCLC, alongside the underlying mechanisms. Human NSCLC cells, A427 and A549, have been handled with curcumin, and the expression ranges of miR‑192‑5p and c‑Myc have been detected utilizing reverse transcription‑quantitative PCR and western blotting. Mobile proliferation was analyzed utilizing Cell Counting Package‑eight assays and cell viability was decided utilizing a MTT assay.

 

Moreover, the migratory and invasive skills of cells have been analyzed utilizing Transwell and Matrigel assays, respectively. The binding websites between miR‑192‑5p and c‑Myc have been predicted utilizing TargetScanHuman software program, and confirmed utilizing a twin‑luciferase reporter assay and RNA immunoprecipitation.

 

Lastly, the Wnt pathway regulator, β‑catenin, and cyclin D1 expression ranges have been decided utilizing western blotting. Curcumin remedy inhibited NSCLC cell proliferation, migration, invasion and viability in a dose‑dependent method, along with selling a dose‑dependent enhance within the expression ranges of miR‑192‑5p and a discount in c‑Myc expression ranges.

 

Notably, the genetic knockdown of miR‑192‑5p blocked the inhibitory results of curcumin on NSCLC development and as an alternative promoted NSCLC development, which was noticed to be partially reversed by c‑Myc silencing; thus, c‑Myc was steered to be a direct goal gene of miR‑192‑5p as demonstrated by the TargetScanHuman database, twin‑lucierase and RIP assay outcomes.

 

As well as, the curcumin‑induced decreased expression ranges of β‑catenin, cyclin D1 and c‑Myc have been rescued following the genetic knockdown of miR‑192‑5p. In conclusion, these findings steered that the upregulation of miR‑192‑5p might underlie the inhibitory results of curcumin on NSCLC cells by concentrating on c‑Myc and inactivating the Wnt/β‑catenin signaling pathway.

 

Mouse Direct PCR Package (For Genotyping)

 

Description: The Mouse Direct PCR Package offers a quick preparation and PCR amplIFication that’s specIFically designed for mouse genotyping. Buffer L and Protease Plus quickly digests mouse tissue to launch intact genomic DNA that can be utilized immediately because the template for PCR amplIFication.

 

The Chinese language natural drugs Qing-Dai (often known as Indigo naturalis) extracted from indigo-bearing vegetation together with Baphicacanthus cusia (Ness) Bremekwas beforehand reported to exhibit anti-psoriatic results in topical remedy.

 

TH17 was later established as a key participant within the pathogenesis of psoriasis. We investigated the anti-TH17 impact of Indigo naturalis and its lively compounds.To judge the toxicity of Indigo naturalis (IN) and its derivatives on 5 cell varieties concerned in psoriasis and to review the anti-inflammatory mechanism for the toxicity.

 

MULTIPLEX KIT PCR Babesia & Theileria PCR package

 

  • Contact us so as to know the reactivity of the package.
Description: An standard PCR package for detection of MULTIPLEX KIT PCR Babesia & Theileria

 

 

Following the fingerprint and amount evaluation of indirubin, indigo, and tryptanthrin in IN extract, we used MTS kits to measure the anti-proliferative impact of IN and three lively compounds on 5 totally different cell varieties recognized in psoriatic lesions.

 

  • Quantitative RT-PCR evaluation was used to measure the expression of assorted genes recognized within the activated keratinocytes and TH17 polarized gene expression in RORγt-expressing T cells.
  • We confirmed that IN differentially inhibited the proliferation of keratinocytes and endothelial cells however not monocytes, fibroblasts nor Jurkat T cells.
  • Amongst three lively compounds recognized in IN, tryptanthrin was probably the most potent compound to scale back their proliferation.
  • Along with differentially lowering IL6 and IL8 expression, each IN and tryptanthrin additionally potently decreased the expression of anti-microbial S100A9 peptide, CCL20 chemokine, IL1B and TNFA cytokines, impartial of NF-κB-p65-activation.
  • Their attenuating impact was additionally detected on the expression of signature cytokines or chemokines induced throughout RORγT-induced TH17 polarization. We have been the primary to verify a direct anti-TH17 impact of each IN natural extract and tryptanthrin.

TuMinute PCR Clean-Up Kit 
P-1005
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  • 100 Samples
  • 50 Samples
  • 100 Samples

PCR DNA CLEAN-UP KIT
IB47035 2 X 96 WELL FORMAT KIT
EUR 305.5

PCR DNA CLEAN-UP KIT
IB47040 4 X 96 WELL FORMAT KIT
EUR 608.35

PCR DNA CLEAN-UP KIT
IB47050 10 X 96 WELL FORMAT KIT
EUR 1425.45

MicroElute PCR Clean Up Kit (200prep)
FAMPK-001-1 200 preps
EUR 224.4

MicroElute PCR Clean Up Kit (50prep)
FAMPK-001 50 preps
EUR 151.2

PCR Clean-Up Mini Kit (200prep)
FAPCK-001-1 200 preps
EUR 202.8

PCR Clean-Up Mini Kit (300prep)
FAPCK-001-2 300 preps
EUR 226.8

PCR Clean-Up Mini Kit (200prep)
FAPCK_001-1 1 kit
EUR 54

PCR Clean-Up Mini Kit (300prep)
FAPCK_001-2 1 kit
EUR 69.5

PCR Clean-Up Mini Kit (50prep)
FAPCK-001 50 preps
EUR 144

PCR Clean-Up Mini Kit (50prep)
FAPCK_001 1 kit
EUR 16

PCR Clean-Up & Gel Extraction Kit
PDC01-0100 100 rxns
EUR 55

PCR Clean-Up & Gel Extraction Kit
MBS402028-100Reactions 100Reactions
EUR 240

PCR Clean-Up & Gel Extraction Kit
MBS402028-4Reactions 4Reactions
EUR 185

PCR Advanced Clean Up System
PF2001 50 preps
EUR 104.58

PCR Advanced Clean Up System
PF2002 250 preps
EUR 427.48

AxyPrep Mag PCR clean up Kit-1mL - EACH
AXY1040 EACH
EUR 102.6

AxyPrep Mag PCR clean up Kit-5mL - EACH
AXY1044 EACH
EUR 410.4

PCR Clean Up for DNA Sequencing
BT5100 100preps
EUR 114.82

PCR Clean Up for DNA Sequencing
BT5101 1000Preps, 1000prep
EUR 553.3

GF-1 PCR Clean-up Kit, 100 preps
GF-PC-100 each Ask for price

GF-1 PCR Clean-up Kit, 200 preps
GF-PC-200 each Ask for price

GF-1 PCR Clean-up Kit, 50 preps
GF-PC-050 each Ask for price

96-well PCR Clean-Up Kit (1 prep)
FACKE-96001 1 prep
EUR 189.6

96-well PCR Clean-Up Kit (2 prep)
FACKE-96002 2 preps
EUR 255.6

96-well PCR Clean-Up Kit (4 prep)
FACKE-96004 4 preps
EUR 380.4

96-well PCR Clean-Up Kit (1 prep)
FACKE_96001 1 kit
EUR 45

96-well PCR Clean-Up Kit (2 prep)
FACKE_96002 1 kit
EUR 87.5

96-well PCR Clean-Up Kit (4 prep)
FACKE_96004 1 kit
EUR 168

Column-Pure Gel and PCR Clean-Up Kit
D516 100 Preparations
EUR 165

GeneMAGNET PCR/ DNA CLEAN - UP Purification Kit
E3420-01 96preps
EUR 138.43

GeneMATRIX PCR/ DNA CLEAN - UP Purification Kit
E3520-01 50preps
EUR 50.14
Description: Kit for purification of PCR products / DNA after enzymatic reactions

GeneMATRIX PCR/ DNA CLEAN - UP Purification Kit
E3520-02 150preps
EUR 138.43
Description: Kit for purification of PCR products / DNA after enzymatic reactions

AXYPREP MAG PCR CLEAN UP KIT- 50 ML - 1110 PREPS
MAG-PCR-CL-50 1/pk
EUR 694.8
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

AXYPREP MAG PCR CLEAN UP KIT- 5 ML - 110 PREPS
MAG-PCR-CL-5 1/pk
EUR 175.2
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

AxyPrep Mag PCR clean up -50mL - EACH
AXY1046 EACH
EUR 1934.55

AXYPREP MAG PCR CLEAN UP KIT- 1 ML - (SAMPLE SIZE)
MAG-PCR-CL-1 1/pk
EUR 69.6
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

MicroElute PCR Clean Up Kit (100prep) (blister packing for columns)
FAMPK_001-1B 1 kit
EUR 56

MicroElute PCR Clean Up Kit (50prep) (blister packing for columns)
FAMPK_001B 1 kit
EUR 28

96-well GE/PCR Clean Up Purification Kit (1 plates)
FAPKE-96001 1 prep
EUR 175.2

96-well GE/PCR Clean Up Purification Kit (2 plates)
FAPKE-96002 2 preps
EUR 225.6

96-well GE/PCR Clean Up Purification Kit (4 plates)
FAPKE-96004 4 preps
EUR 328.8

96-well GE/PCR Clean Up Purification Kit (1 plates)
FAPKE_96001 1 kit
EUR 36

96-well GE/PCR Clean Up Purification Kit (2 plates)
FAPKE_96002 1 kit
EUR 68.5

96-well GE/PCR Clean Up Purification Kit (4 plates)
FAPKE_96004 1 kit
EUR 134.5

PAGE Clean Up Kit
06441-50 1KIT
EUR 269.5

DNA Clean-up Kit
MBS278006-200Preps 200Preps
EUR 250

DNA Clean-up Kit
MBS278006-50Preps 50Preps
EUR 180

GF-1 96-well PCR Clean-up Kit, 96 x 5 plates
GF-96-C05 each Ask for price

GF-1 96-well PCR Clean-up Kit, 96 x 10 plates
GF-96-C10 each Ask for price

PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System)
60200 50 Preps
EUR 83.4

Universal RNA Clean-Up Kit
E3589-01 25preps
EUR 62.13
Description: Kit removes DNA and other contaminants from RNA samples isolated from different sources (animal and plant tissues, bacteria, yeast, blood etc.)

Universal RNA Clean-Up Kit
E3589-02 100preps
EUR 234.35
Description: Kit removes DNA and other contaminants from RNA samples isolated from different sources (animal and plant tissues, bacteria, yeast, blood etc.)