An Optimised Direct Lysis Technique for Viral RNA Extraction and Detection of Foodborne Viruses on Fruits and Greens

FastAmp™ Plant Direct PCR Kit - 1,250 Reactions
4615	Intact Genomics	1/EA	EUR 495
Description: Intact Genomics FastAmpTM Plant Direct PCR kit is an easy and robust method to amplify DNA fragments from plant tissues with high specificity and high sensitivity without the need of complicated DNA purification steps. The advanced formulation of this kit also allows fast PCR cycling conditions without compromising PCR sensitivity, specificity and yield. The FastAmpTM Plant Direct PCR kit is an ideal and powerful tool for high-throughput genotyping, DNA amplification, and plant genome analysis.Highlights:Direct PCR- no need to purify DNASpecially engineered Taq DNA polymerase with highest sensitivity and specificityExtremely short PCR protocol timesMaster mix format with premixed gel loading dye to reduce cross-contamination and sample handlingerrors5x magic enhancer for high GC containing DNA amplificationProduct Includes:FastAmp™ plant direct PCR master mix (2x)Dilution bufferControl primer mix (25 µM each)5x magic enhancerNuclease- free water

FastAmp® Plant Direct PCR & Genotyping Solution - 20mL
4611	Intact Genomics	20mL	EUR 195
Description: FastAmp® Plant Direct PCR/Genotyping Solution for is designed for rapid cell lysis and DNA separation simultaneously without the need of DNA extraction steps . It contains very low concentration of less toxic chemicals for direct DNA amplification of genomic DNA from various plant tissues. No heat treatment and no termination buffer is is required, saving significant time. FastAmp® Plant Direct PCR/Genotyping Solution has been optimized with FastAmp® Plant Direct PCR kit and FastAmp® Plant Tissue/Seed Genotyping PCR kit.

Direct PCRKits for Plant
FYT303-100P	Yeastern Biotech	100 Preps	Ask for price

Phire Plant Direct PCR Kit (without sampling tools) - 200 x 50 µL rxns
THF-130WH	Westburg	each	EUR 323.73

Phire Plant Direct PCR Master Mix - 500 x 50 µl rxns
THF-160L	Westburg	each	EUR 648.55

Phire Plant Direct PCR Master Mix - 100 x 50 µl rxns
THF-160S	Westburg	each	EUR 165.68

Direct PCR Complete Kit
S700	GeneOn	100 rcs	EUR 159.6

Direct PCR Complete Kit
S700-2	GeneOn	5x100 rcs	EUR 628.8

Direct Blood PCR Kit
MBPCR171-100R	EWC Diagnostics	1 unit	EUR 267.52
Description: Direct Blood PCR Kit

Direct Blood PCR Kit
MBPCR171-10R	EWC Diagnostics	1 unit	EUR 71.73
Description: Direct Blood PCR Kit

Direct Blood PCR Kit
E0950-01	EURx	100reactions	EUR 69.76
Description: Enables to perform PCR reactions directly from whole blood with no prior DNA extraction or purification

Direct Blood PCR Kit
E0950-02	EURx	500reactions	EUR 277.95
Description: Enables to perform PCR reactions directly from whole blood with no prior DNA extraction or purification

Direct Tissue PCR Kit
E0940-01	EURx	100reactions	EUR 55.59
Description: Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no priorDNA purification

Direct Tissue PCR Kit
E0940-02	EURx	500reactions	EUR 251.79
Description: Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no priorDNA purification

Blood Direct PCR Kit V2
PD103-01	Vazyme	50 rxn	EUR 51.41

Blood Direct PCR Kit V2
PD103-02	Vazyme	200 rxn	EUR 188.5

Mouse tissue direct PCR kit
EP033-200T	ELK Biotech	200T	EUR 125

Mouse tissue direct PCR kit
EP033-50T	ELK Biotech	50T	EUR 40

Mouse tissue direct PCR kit
EP033	ELK Biotech	50T	EUR 125

Mouse Tissue Direct PCR Kit
RK20736	Abclonal	100RXN	EUR 150

Mouse Direct PCR Kit (For Genotyping)
B40013	Bimake	200 ractions	EUR 250.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B40015	Bimake	500 ractions	EUR 490.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B45012	Bimake	1ml*5 (500 ractions)	EUR 336
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B45013	Bimake	1ml*20 (2000 ractions)	EUR 1038
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Plant Preservative Mixture
PCT01	Plant Cell Technology	30 ml	EUR 108
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture
PCT02	Plant Cell Technology	100 ml	EUR 898.8
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture
PCT03	Plant Cell Technology	250 ml	EUR 372
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture
PCT04	Plant Cell Technology	500 ml	EUR 540
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

Plant Preservative Mixture PPM
PCT05	Plant Cell Technology	1000 ml	EUR 958.8
Description: Plant Preservative Mixture, also known as PPM, is a powerful biocide against waterborne, endogenous contamination, airborne, fungal contamination and human contact. It is a liquid solution that prevents and reduces microbial contamination in plant tissue culture, and it is an excelent replacement of the antibiotics. It targets and inhibits multiple enzymes, the formation of resistant mutants towards PPM is unlikely. It is used both as a biocide and biostatic compound as a preventative measure.

BioLit Animal Tissue Direct PCR Kit (Research)
91210	Sisco Laboratories	50 Preps	EUR 222.28
Description: Part E

Allele-In-One Mouse Tail Direct PCR Kit
ABP-PP-MT02100	Allele Biotech	100 RXNS	Ask for price

Allele-In-One Mouse Tail Direct PCR Kit
ABP-PP-MT02500	Allele Biotech	500 RXNS	Ask for price

amfiSure Direct PCR Lysis Buffer
D0300-010	GenDepot	100ml	EUR 165.6

Supreme Plant Tissue Culture Grade Agar
PCTA100	Plant Cell Technology	500g	EUR 144
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Supreme Plant Tissue Culture Grade Agar
PCTA500	Plant Cell Technology	1000g	EUR 180
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Allele-In-One Human Blood Direct PCR Kit
ABP-PP-BD02100	Allele Biotech	100 RXNS	Ask for price

Allele-In-One Human Blood Direct PCR Kit
ABP-PP-BD02500	Allele Biotech	500 RXNS	Ask for price

Phusion Blood Direct PCR Kit - 500 x 20 µL rxns
THF-547L	Westburg	each	EUR 583.15

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)
105-540	GeneOn	20 rcs x 50 µl	EUR 196.8

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)
105-542	GeneOn	100 rcs x 50 µl	EUR 658.8

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)
105-544	GeneOn	1000 rcs x 50 µl	EUR 5026.8

Phusion Human Specimen Direct PCR Kit - 200 x 20 µL rxns
THF-150	Westburg	each	EUR 348.26

Phire Animal Direct PCR Kit (without sampling tools) - 200 x 50 µL rxns
THF-140WH	Westburg	each	EUR 412.57

Direct RT-PCR Kit wioRealStart DNA Polymerase
FYT507-100P	Yeastern Biotech	100 Preps	Ask for price

Direct RT-PCR Kit wioRealStart DNA Polymerase
FYT507-50P	Yeastern Biotech	50 Preps	Ask for price

Phire Tissue Direct PCR Master Mix - 500 x 50 µl rxns
THF-170L	Westburg	each	EUR 888.35

Phire Tissue Direct PCR Master Mix - 100 x 50 µl rxns
THF-170S	Westburg	each	EUR 203.29

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miR‑192‑5p upregulation mediates the suppression of curcumin in human NSCLC cell proliferation, migration and invasion by concentrating on c‑Myc and inactivating the Wnt/β‑catenin signaling pathway

Curcumin is a naturally lively phenolic compound extracted from the rhizome of the plant Curcuma longa, which has been demonstrated to function an anticancer drug in various kinds of most cancers, together with non‑small‑cell lung most cancers (NSCLC). Accumulating proof has steered that curcumin might exert epigenetic regulatory results on microRNAs (miRs).

Subsequently, the current examine aimed to research the position of miR‑192‑5p, and the consequences of curcumin, in NSCLC, alongside the underlying mechanisms. Human NSCLC cells, A427 and A549, have been handled with curcumin, and the expression ranges of miR‑192‑5p and c‑Myc have been detected utilizing reverse transcription‑quantitative PCR and western blotting. Mobile proliferation was analyzed utilizing Cell Counting Package‑eight assays and cell viability was decided utilizing a MTT assay.

Moreover, the migratory and invasive skills of cells have been analyzed utilizing Transwell and Matrigel assays, respectively. The binding websites between miR‑192‑5p and c‑Myc have been predicted utilizing TargetScanHuman software program, and confirmed utilizing a twin‑luciferase reporter assay and RNA immunoprecipitation.

Lastly, the Wnt pathway regulator, β‑catenin, and cyclin D1 expression ranges have been decided utilizing western blotting. Curcumin remedy inhibited NSCLC cell proliferation, migration, invasion and viability in a dose‑dependent method, along with selling a dose‑dependent enhance within the expression ranges of miR‑192‑5p and a discount in c‑Myc expression ranges.

Notably, the genetic knockdown of miR‑192‑5p blocked the inhibitory results of curcumin on NSCLC development and as an alternative promoted NSCLC development, which was noticed to be partially reversed by c‑Myc silencing; thus, c‑Myc was steered to be a direct goal gene of miR‑192‑5p as demonstrated by the TargetScanHuman database, twin‑lucierase and RIP assay outcomes.

As well as, the curcumin‑induced decreased expression ranges of β‑catenin, cyclin D1 and c‑Myc have been rescued following the genetic knockdown of miR‑192‑5p. In conclusion, these findings steered that the upregulation of miR‑192‑5p might underlie the inhibitory results of curcumin on NSCLC cells by concentrating on c‑Myc and inactivating the Wnt/β‑catenin signaling pathway.

MULTIPLEX KIT PCR Babesia & Theileria PCR package