Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Dec 10, 2020 Assay Kits

Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Background and goal: Flinders Expertise Associates (FTA) playing cards simplify pattern storage, transport, and extraction by decreasing price and time for analysis. This examine evaluated the FTA suitability for protected transport and storage of Gram-positive and Gram-negative bacterial cells of animal origin on its liquid tradition type and from organ impression smears (tissues) underneath the identical routine situation of microbiological laboratory together with detecting their nucleic acid over completely different storage circumstances.
Supplies and strategies: Enhance in bacterial depend from 104 to 107 (colony-forming items/mL) of 78 isolates representing seven bacterial species was utilized onto playing cards. FTA playing cards had been grouped and inoculated by these micro organism after which saved at completely different circumstances of 24-27°C, 4°C, and -20°C for 24 h, for two weeks, for 1 and three month storage, respectively. Bacteriological examination was completed, after which bacterial DNA was recognized utilizing particular primers for every bacterial kind and detected by polymerase chain response (PCR).
Outcomes: The whole share of recovered micro organism from FTA playing cards was 66.7% at 24-27°C for 24 h, the detection restrict was 100% in Gram-positive species, whereas it was 57.4% in Gram-negative ones. Relating to viable cell detection from organ impression smears, it was profitable underneath the earlier circumstances. No reside bacterial cells had been noticed by bacteriological isolation quite than solely at 24-27°C for 24 h storage. All bacterial DNA had been sufficiently confirmed by the PCR approach at completely different circumstances.
Conclusion: General, the FTA card technique was noticed to be a legitimate software for nucleic acid purification for micro organism of animal origin within the type of tradition or organ smears no matter its Gram kind and is used for a short while solely 24 h for storage and transport of reside micro organism particularly Gram-positive kind. Furthermore, the bacterial nucleic acid was intact after storage in -20°C for three months and was PCR amplifiable.

An environment friendly technique for the development of synthetic, concatemeric DNA, RNA and proteins with genetically programmed features, utilizing a novel, vector-enzymatic DNA fragment amplification-expression know-how

De novo designed bioactive molecules, equivalent to DNA, RNA and peptides, are utilized in more and more various scientific, industrial and biomedical functions. Concatemerization of designed DNA, RNA and peptides might enhance their stability, bioactivity and permit for gradual launch of the bioactive molecule on the supposed vacation spot. On this context, we developed a brand new technique enabling the formation of DNA concatemers for the manufacturing of synthetic, repetitive genes, encoding concatemeric RNAs and proteins of any nucleotide and amino-acid sequence.
The know-how recruits the Sort IIS SapI restriction endonuclease (REase) for assembling DNA fragments in an ordered head-to-tail-orientation. Alternatively, different commercially out there SapI isoschizomers can be utilized: LguI and thermostable BspQI. 4 sequence of DNA vectors devoted to the expression of newly shaped, concatemeric open studying frames (ORFs), had been designed and constructed to satisfy the know-how wants. • Vector-enzymatic DNA fragment amplification know-how. • Building of DNA concatemers many occasions longer than these out there with the usage of present de novo gene synthesis strategies. • Biosynthesis of protein tandem repeats with programmable operate by no means seen in nature. With the fast improvement of isothermal amplification know-how, DNA molecular analysis has grow to be an necessary reference for scientific remedy.
On this work, we’ve designed a DNA molecular diagnostic know-how with LAMP-like sensitivity for nucleic acid evaluation and detection based mostly on just one pair of hairpin primers. This DNA molecular diagnostic know-how consists of Bst DNA polymerase and one pair of hairpin primers, that are designed simply by including a stem-loop construction to a goal binding area. When the goal is current, the polymerization response between the hairpin primers and the goal generates a particular dumbbell DNA much like LAMP, which triggers cyclic amplification reactions to increase a sequence of lengthy dsDNA merchandise with repeated sequences by inserting fluorescent dye Eva Inexperienced noticed the rise in fluorescence sign.
Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions
Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Applied sciences for focused DNA methylation modifications in most cancers:Mechanism and utility

DNA methylation abnormalities are thought to be important occasion for most cancers initiation and improvement. Tumor-associated genes encompassing aberrant DNA methylation alterations at particular locus are correlated with gene silencing and chromatin reworking in varied malignancies. Thus, applied sciences designed to govern DNA methylation at particular loci of genome are crucial for the useful examine and therapeutic utility within the context of most cancers administration.

AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria

Z1322006 1 kit (48 extractions) Including all required plastic disposables
EUR 216

Bacterial DNA Extraction Kit (Proteinase K included)

BA50 50 preps
EUR 120

Bacterial DNA Extraction Kit (Proteinase K included)

BA05 5 preps Ask for price

Bacterial DNA Extraction Kit (Proteinase K included)

BA100 100 preps
EUR 165.6

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS423 50Preps
EUR 98.36

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS624 250preps
EUR 231.86

EasyRNA? Bacterial RNA Kit

K1351-250 each
EUR 1161.6

EasyRNA? Bacterial RNA Kit

K1351-50 each
EUR 444

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 186

Bacterial Flagellin

VAdv-Ly0027 50 µg
EUR 9391.2
Description: Bacterial flagellin, a TLR5 agonist vaccine adjuvant.

Bacterial Collagenase Assay Kit

3014 1 kit
EUR 700.32
Description: Bacterial Collagenase Assay Kit

Ricketssia like bacterial PCR kit

PCR-V283-48D 50T
EUR 543.6

Ricketssia like bacterial PCR kit

PCR-V283-96D 100T
EUR 686.4

Bacterial Recombinant MDH

6357-100 each
EUR 574.8

Bacterial Recombinant GOR

6380-100 each
EUR 548.4

Agar Bacterial Tech 2Kg

281210 EACH
EUR 686.4

NFNB, bacterial recombinant

7830-100 each
EUR 483.6

Bacterial Recombinant SurA

6381-100 each
EUR 548.4

Ricketssia like bacterial RT PCR kit

RTq-V283-100D 100T
EUR 860.4

Ricketssia like bacterial RT PCR kit

RTq-V283-150D 150T
EUR 969.6

Ricketssia like bacterial RT PCR kit

RTq-V283-50D 50T
EUR 717.6

MycoLightâ„¢ Bacterial Viability Assay Kit

22400-200Tests 200 Tests
EUR 334
Description: AAT Bioquest's Mycolight™ Bacterial Viability Assay Kit provides two-color fluorescence assay of bacterial viability in both gram-positive and negative bacterial cell.

Human bacterial vaginosis,BV ELISA Kit

201-12-1087 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Bacterial vaginosis (BV) ELISA Kit

abx052798-96tests 96 tests
EUR 801.6

Human bacterial vaginosis(BV)ELISA Kit

GA-E1103HM-48T 48T
EUR 346.8

Human bacterial vaginosis(BV)ELISA Kit

GA-E1103HM-96T 96T
EUR 559.2

Human bacterial vaginosis(BV)ELISA Kit

QY-E00922 96T
EUR 433.2

Human bacterial vaginosis,BV ELISA Kit

YLA2512HU-48T 48T
EUR 435

Human bacterial vaginosis,BV ELISA Kit

YLA2512HU-96T 96T
EUR 562.5

Collagenase (bacterial) antibody

20C-CR6033RP 500 ul
EUR 516
Description: Rabbit polyclonal Collagenase (bacterial) antibody

Bacterial Counting Colorimetric Assay Kit

K511-2500 each
EUR 744

Bacterial Counting Colorimetric Assay Kit

K511-500 each
EUR 418.8

Live Bacterial Gram Stain Kit - 200 assays

32000-1 1kit
EUR 228
Description: Minimum order quantity: 1 unit of 1kit

MycoLight™ Bacterial Viability Assay Kit

22400 200 Tests
EUR 334

Ricketssia like bacterial One-Step PCR kit

Oneq-V283-100D 100T
EUR 1039.2

Ricketssia like bacterial One-Step PCR kit

Oneq-V283-150D 150T
EUR 1177.2

Ricketssia like bacterial One-Step PCR kit

Oneq-V283-50D 50T
EUR 861.6

Bacterial Protein Extraction Buffer

20010 200 ml
EUR 130
Description: BPS Bacterial Protein extraction Buffer enables extraction of both soluble and insoluble (inclusion bodies) proteins from bacteria. The buffer provides high efficiency recovery of over-expressed proteins and is fully compatible with HIS/GST-tag purification procedures and standard protein concentration determination assays. The buffer can also be used for protein extraction from insect cells.

Bacterial Protein Extraction Buffer

20020 500 ml
EUR 245
Description: BPS Bacterial Protein extraction Buffer enables extraction of both soluble and insoluble (inclusion bodies) proteins from bacteria. The buffer provides high efficiency recovery of over-expressed proteins and is fully compatible with HIS/GST-tag purification procedures and standard protein concentration determination assays. The buffer can also be used for protein extraction from insect cells.

ATCC16404 Standard bacterial Strains

S0080 100 ul
EUR 438

ATCC13813 Standard bacterial Strains

S0092 100 ul
EUR 438

ATCC27853 Standard bacterial Strains

S0110 100 ul
EUR 438

ATCC10792 Standard bacterial Strains

S0111 100 ul
EUR 438

ATCC19606 Standard bacterial Strains

S0113 100 ul
EUR 438

ATCC25922 Standard bacterial Strains

S0077 100 ul
EUR 438

ATCC25923 Standard bacterial Strains

S0078 100 ul
EUR 438

ATCC17802 Standard bacterial Strains

S0079 100 ul
EUR 438

3-min Bacterial Total Protein Extraction Kit

P512L - Ask for price

Suez Filter Cartridge Bacterial 0.1um

WAT2274 EACH
EUR 960

Bacterial Viability and Gram Stain Kit - 200 assays

32001 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Sushi Peptide S3, bacterial recombinant

7611-100 each
EUR 444

Sushi Peptide S3, bacterial recombinant

7611-1000 each
EUR 1227.6

Sushi Peptide S3, bacterial recombinant

7611-20 each
EUR 183.6

CMCC (F) 98003 Standard bacterial Strains

S0088 100 ul
EUR 438

CMCC (B) 44102 Standard bacterial Strains

S0096 100 ul
EUR 438

CMCC (B) 10104 Standard bacterial Strains

S0097 100 ul
EUR 438

CMCC (B) 51572 Standard bacterial Strains

S0081 100 ul
EUR 438

CMCC (B) 26003 Standard bacterial Strains

S0083 100 ul
EUR 438

CMCC (F) 98001 Standard bacterial Strains

S0084 100 ul
EUR 438

CMCC (B) 54002 Standard bacterial Strains

S0085 100 ul
EUR 438

Bacterial Sortase Substrate III, Abz/DNP

HY-P1883 5mg
EUR 782.4

ReadiUseâ„¢ bacterial cell lysis buffer *5X*

24100-10mL 10 mL
EUR 109
Description: Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS585 5XPlates, 480prep
EUR 585.38

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS5852 2XPlates, 192prep
EUR 300.37

MycoLightâ„¢ Rapid Fluorescence Bacterial Gram Stain Kit

22413-100Tests 100 Tests
EUR 446
Description: The MycoLightâ„¢ Rapid Fluorescence Bacterial Gram Stain Kit provides an easy and convenient way for determination of gram sign in live bacteria.

EZLys? Bacterial Protein Extraction Reagent

8001-100 each
EUR 157.2

EZLys? Bacterial Protein Extraction Reagent

8001-500 each
EUR 352.8

Bacterial Total Protein Extraction Reagent

abx090632-50100assays 50-100 assays
EUR 309.6

MycoLightâ„¢ Fluorescence Live/Dead Bacterial Imaging Kit

22411-100Tests 100 Tests
EUR 446
Description: AAT Bioquest's MycoLight™ Fluorescence Live/Dead Bacterial Imaging Kit provides two-color fluorescence assay for visualizing live and dead bacteria through fluorescent microscope.

ReadiUse™ bacterial cell lysis buffer *5X*

24100 10 mL
EUR 109

Native Bacterial Phytase, feed grade, 50,000U/g

NATE-0565 5kg
EUR 855.6

Complete Bacterial Protein Extraction Reagent

AR0157 50mL (sufficient for lysis of 10g of bacterial biomass)
EUR 169.2

MycoLight™ Rapid Fluorescence Bacterial Gram Stain Kit

22413 100 Tests
EUR 446

Bacterial Outer Membrane Protein-A Protein

20-abx263222
  • EUR 577.20
  • EUR 2148.00
  • EUR 693.60
  • 100 ug
  • 1 mg
  • 200 ug

5X Bacterial Protein Extraction Reagent (Tris)

AKR-180 50mL
EUR 331.2
Description: The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption.

pC001 - huLshC2C2- MBP for bacterial expression

PVT10645 2 ug
EUR 361.2

Bacterial Sortase Substrate III, Abz/DNP (TFA)

HY-P1883A 1mg
EUR 300

Acetobacter xylinum Standard bacterial Strains

S0090 100 ul
EUR 438

MycoLight™ Fluorescence Live/Dead Bacterial Imaging Kit

22411 100 Tests
EUR 446

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA)

31050 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

Recombinant Bacterial Outer Membrane Protein-A

7-05761 100µg Ask for price

Recombinant Bacterial Outer Membrane Protein-A

7-05762 200µg Ask for price

Recombinant Bacterial Outer Membrane Protein-A

7-05763 1mg Ask for price

Oxalate Decarboxylase, Active Bacterial Recombinant

7262-100 each
EUR 535.2

Oxalate Decarboxylase, Active Bacterial Recombinant

7262-1000 each
EUR 3517.2

Oxalate Decarboxylase, Active Bacterial Recombinant

7262-20 each
EUR 294

5X Bacterial Protein Extraction Reagent (Phosphate)

AKR-181 50mL
EUR 331.2
Description: The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption.

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) PMAxx

31050-X 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

Bacterial Protein Lysis Buffer, 1x Stength Solution

BSP023-25ml 25ml, 20prep
EUR 91.32

Bacterial Protein Lysis Buffer, 1x Stength Solution

BSP023-4x25ml 4x25ml, 80prep
EUR 174.84

Bacterial Lipopolysaccharides (LPS) Extraction Kit (up to 50 samples)

1000-100-LPS 1 kit
EUR 489.6

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276)

31037 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

Superoxide Dismutase A (SOD-A), bacterial recombinant

6361-100 each
EUR 548.4

Bacteria Genomic DNA Kit

20-abx098080
  • EUR 126.00
  • EUR 292.80
  • 2 rxns
  • 50 rxns

Helicobacter pylori Bacterial non-heme ferritin (ftnA)

1-CSB-BP009053HUV
  • EUR 1008.00
  • EUR 398.40
  • EUR 2604.00
  • EUR 1362.00
  • EUR 1894.80
  • EUR 564.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Helicobacter pylori Bacterial non-heme ferritin(ftnA) expressed in Baculovirus

Helicobacter pylori Bacterial non-heme ferritin (ftnA)

1-CSB-YP009053HUV
  • EUR 814.80
  • EUR 402.00
  • EUR 2606.40
  • EUR 1261.20
  • EUR 1730.40
  • EUR 522.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Helicobacter pylori Bacterial non-heme ferritin(ftnA) expressed in Yeast

Helicobacter pylori Bacterial non-heme ferritin (ftnA)

1-CSB-EP009053HUV
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Helicobacter pylori Bacterial non-heme ferritin(ftnA) expressed in E.coli

Bacterial RNA Polymerase Pyrophosphate Assay Kit (enzyme not included)

RPAP100K 100 assays
EUR 278.4
Description: This product includes 400 ul of Buffer RP, 33 ul of 100 x DNA, 33 ul of 100 x NTP mix, 5 ul of 1000 x pyrophosphatase and 4500 ul of dye for 100 assays of E. coli RNA polymerase reactions in a 384-well assay format. The assay This product includes all reagents except the enzyme.

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA)

31036 1kit
EUR 478.8
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly)

31051 1kit
EUR 478.8
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip)

31053 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

Extract-EZ B, Bacterial Protein Extraction Kit, 10X Strength Solution

BS596 1kit, 10prep
EUR 98.63

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) PMAxx

31037-X 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) PMAxx

31033-X 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

Recombinant (E.Coli) Bacterial Outer Membrane Protein-A (Omp-A)

RP-981 50 ug
EUR 343.2

QPCR Kit DNA Bacteria Domain

MOL6430 EACH
EUR 842.46

Bacterial/Permeability-Increasing Protein (BPI), Human Neutrophil

BPI15-N-50 50 ug
EUR 270

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) PMAxx

31051-X 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

MycoLightâ„¢ Ratiometric Bacterial Membrane Potential Kit *Red/Green Fluorescence*

22401-200Tests 200 Tests
EUR 334
Description: AAT Bioquest's MycoLight™ Ratiometric Bacterial Membrane Potential Kit uses a fluorescent sensor that exhibits green fluorescence in both gram-positive and negative bacterial cells in low concentration, but the fluorescence shifts toward red emission at higher cytosolic concentrations due to the dye molecule aggregation caused by larger membrane potentials.

Rabbit Anti Bacterial Alkaline Phosphatase Polyclonal Antibody,HRP

CPBT-66804RB 0.5 mg
EUR 1182

MycoLight™ Ratiometric Bacterial Membrane Potential Kit *Red/Green Fluorescence*

22401 200 Tests
EUR 334

QPCR Kit DNA Bacteria DomaiMmix

MOL6432 EACH
EUR 1072.74

miRZip-1246 anti-miR-1246 microRNA construct bacterial streak

MZIP1246-PA-1 Bacterial Streak
EUR 620

miRZip-1248 anti-miR-1248 microRNA construct bacterial streak

MZIP1248-PA-1 Bacterial Streak
EUR 620
Historically, the manipulation of DNA methylation demonstrates an unspecific function, adversely inflicting global-genome epigenetic alterations and complicated the operate function of desired gene on epigenetic regulation. Novel applied sciences for focused DNA methylation regulation have an ideal benefit of manipulating gene epigenetic alterations in a extra particular and environment friendly technique. On this evaluation, we described completely different site-specific concentrating on DNA methylation methods, together with each their benefits and limitations. By a complete understanding of those concentrating on instruments, we hope to open a brand new perspective for most cancers remedy.

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