Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Dec 10, 2020 Assay Kits

Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Background and goal: Flinders Expertise Associates (FTA) playing cards simplify pattern storage, transport, and extraction by decreasing price and time for analysis. This examine evaluated the FTA suitability for protected transport and storage of Gram-positive and Gram-negative bacterial cells of animal origin on its liquid tradition type and from organ impression smears (tissues) underneath the identical routine situation of microbiological laboratory together with detecting their nucleic acid over completely different storage circumstances.
Supplies and strategies: Enhance in bacterial depend from 104 to 107 (colony-forming items/mL) of 78 isolates representing seven bacterial species was utilized onto playing cards. FTA playing cards had been grouped and inoculated by these micro organism after which saved at completely different circumstances of 24-27°C, 4°C, and -20°C for 24 h, for two weeks, for 1 and three month storage, respectively. Bacteriological examination was completed, after which bacterial DNA was recognized utilizing particular primers for every bacterial kind and detected by polymerase chain response (PCR).
Outcomes: The whole share of recovered micro organism from FTA playing cards was 66.7% at 24-27°C for 24 h, the detection restrict was 100% in Gram-positive species, whereas it was 57.4% in Gram-negative ones. Relating to viable cell detection from organ impression smears, it was profitable underneath the earlier circumstances. No reside bacterial cells had been noticed by bacteriological isolation quite than solely at 24-27°C for 24 h storage. All bacterial DNA had been sufficiently confirmed by the PCR approach at completely different circumstances.
Conclusion: General, the FTA card technique was noticed to be a legitimate software for nucleic acid purification for micro organism of animal origin within the type of tradition or organ smears no matter its Gram kind and is used for a short while solely 24 h for storage and transport of reside micro organism particularly Gram-positive kind. Furthermore, the bacterial nucleic acid was intact after storage in -20°C for three months and was PCR amplifiable.

An environment friendly technique for the development of synthetic, concatemeric DNA, RNA and proteins with genetically programmed features, utilizing a novel, vector-enzymatic DNA fragment amplification-expression know-how

De novo designed bioactive molecules, equivalent to DNA, RNA and peptides, are utilized in more and more various scientific, industrial and biomedical functions. Concatemerization of designed DNA, RNA and peptides might enhance their stability, bioactivity and permit for gradual launch of the bioactive molecule on the supposed vacation spot. On this context, we developed a brand new technique enabling the formation of DNA concatemers for the manufacturing of synthetic, repetitive genes, encoding concatemeric RNAs and proteins of any nucleotide and amino-acid sequence.
The know-how recruits the Sort IIS SapI restriction endonuclease (REase) for assembling DNA fragments in an ordered head-to-tail-orientation. Alternatively, different commercially out there SapI isoschizomers can be utilized: LguI and thermostable BspQI. 4 sequence of DNA vectors devoted to the expression of newly shaped, concatemeric open studying frames (ORFs), had been designed and constructed to satisfy the know-how wants. • Vector-enzymatic DNA fragment amplification know-how. • Building of DNA concatemers many occasions longer than these out there with the usage of present de novo gene synthesis strategies. • Biosynthesis of protein tandem repeats with programmable operate by no means seen in nature. With the fast improvement of isothermal amplification know-how, DNA molecular analysis has grow to be an necessary reference for scientific remedy.
On this work, we’ve designed a DNA molecular diagnostic know-how with LAMP-like sensitivity for nucleic acid evaluation and detection based mostly on just one pair of hairpin primers. This DNA molecular diagnostic know-how consists of Bst DNA polymerase and one pair of hairpin primers, that are designed simply by including a stem-loop construction to a goal binding area. When the goal is current, the polymerization response between the hairpin primers and the goal generates a particular dumbbell DNA much like LAMP, which triggers cyclic amplification reactions to increase a sequence of lengthy dsDNA merchandise with repeated sequences by inserting fluorescent dye Eva Inexperienced noticed the rise in fluorescence sign.
Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions
Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Applied sciences for focused DNA methylation modifications in most cancers:Mechanism and utility

DNA methylation abnormalities are thought to be important occasion for most cancers initiation and improvement. Tumor-associated genes encompassing aberrant DNA methylation alterations at particular locus are correlated with gene silencing and chromatin reworking in varied malignancies. Thus, applied sciences designed to govern DNA methylation at particular loci of genome are crucial for the useful examine and therapeutic utility within the context of most cancers administration.
Bacterial DNA Purification Kit II
K1457-250 250 Preps
EUR 581
Bacterial DNA Purification Kit II
K1457-50 50 Preps
EUR 318
Milk Bacterial DNA Extraction Kit (50prep)
FAMBD-001 50 preps
EUR 274
100 BP DNA LADDER, 500UL PER KIT
M-DNA-100BP 1/pk
EUR 73
Description: Bioscience Mol Bio; DNA Ladder
1 KB DNA LADDER, 500UL PER KIT
M-DNA-1KB 1/pk
EUR 70
Description: Bioscience Mol Bio; DNA Ladder
Bacterial DNA Extraction Kit (Proteinase K included)
BA05 5 preps Ask for price
Bacterial DNA Extraction Kit (Proteinase K included)
BA100 100 preps
EUR 138
Bacterial DNA Extraction Kit (Proteinase K included)
BA50 50 preps
EUR 100
EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS423 50Preps
EUR 81.97
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria
EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS624 250preps
EUR 193.22
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria
Bacterial Flagellin
VAdv-Ly0027 50 µg
EUR 7826
Description: Bacterial flagellin, a TLR5 agonist vaccine adjuvant.
Bacterial Collagenase Assay Kit
3014 1 kit
EUR 583.6
Description: Bacterial Collagenase Assay Kit
EasyRNA? Bacterial RNA Kit
K1351-250
EUR 968
EasyRNA? Bacterial RNA Kit
K1351-50
EUR 370
Bacterial RNA Extraction Kit
R403-01 100 rxn
EUR 155
Bacterial Counting Colorimetric Assay Kit
K511-2500
EUR 620
Bacterial Counting Colorimetric Assay Kit
K511-500
EUR 349
Ricketssia like bacterial PCR kit
PCR-V283-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Ricketssia like bacterial
Ricketssia like bacterial PCR kit
PCR-V283-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Ricketssia like bacterial
NFNB, bacterial recombinant
7830-100
EUR 403
Collagenase (bacterial) antibody
20C-CR6033RP 500 ul
EUR 430
Description: Rabbit polyclonal Collagenase (bacterial) antibody
Bacterial Recombinant MDH
6357-100
EUR 479
Bacterial Recombinant GOR
6380-100
EUR 457
Bacterial Recombinant SurA
6381-100
EUR 457
Frit Kit
FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.
Human bacterial vaginosis(BV)ELISA Kit
GA-E1103HM-48T 48T
EUR 289
Human bacterial vaginosis(BV)ELISA Kit
GA-E1103HM-96T 96T
EUR 466
Human Bacterial vaginosis (BV) ELISA Kit
abx052798-96tests 96 tests
EUR 668
  • Shipped within 5-10 working days.
Human bacterial vaginosis,BV ELISA Kit
201-12-1087 96 tests
EUR 440
  • This bacterial vaginosis ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.
Ricketssia like bacterial RT PCR kit
RTq-V283-100D 100T
EUR 628.5
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Ricketssia like bacterial .
Ricketssia like bacterial RT PCR kit
RTq-V283-150D 150T
EUR 701
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Ricketssia like bacterial .
Ricketssia like bacterial RT PCR kit
RTq-V283-50D 50T
EUR 532.8
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Ricketssia like bacterial .
Human bacterial vaginosis(BV)ELISA Kit
QY-E00922 96T
EUR 361
Column Packing Kit
PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.
ATCC25922 Standard bacterial Strains
S0077 100 ul
EUR 365
ATCC25923 Standard bacterial Strains
S0078 100 ul
EUR 365
ATCC17802 Standard bacterial Strains
S0079 100 ul
EUR 365
ATCC16404 Standard bacterial Strains
S0080 100 ul
EUR 365
ATCC13813 Standard bacterial Strains
S0092 100 ul
EUR 365
ATCC27853 Standard bacterial Strains
S0110 100 ul
EUR 365
ATCC10792 Standard bacterial Strains
S0111 100 ul
EUR 365
ATCC19606 Standard bacterial Strains
S0113 100 ul
EUR 365
PCR Mycoplasma Detection Kit
M034-Kit Kit
EUR 266
Live Bacterial Gram Stain Kit - 800 assays
32000 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit
Live Bacterial Gram Stain Kit - 200 assays
32000-1 1kit
EUR 190
Description: Minimum order quantity: 1 unit of 1kit
Cell Meter™ Bacterial Viability Assay Kit
22400 200 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200
Ricketssia like bacterial One-Step PCR kit
Oneq-V283-100D 100T
EUR 747.4
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ricketssia like bacterial.
Ricketssia like bacterial One-Step PCR kit
Oneq-V283-150D 150T
EUR 838.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ricketssia like bacterial.
Ricketssia like bacterial One-Step PCR kit
Oneq-V283-50D 50T
EUR 628.5
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ricketssia like bacterial.
3-min Bacterial Total Protein Extraction Kit
P512L NULL
EUR 0
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS400A-KIT 1 kit (10 rxn)
EUR 1110
  • Category: Cas9
CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV100PA-KIT 1 Kit
EUR 1132
  • Category: Cas9
CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV105PA-KIT 1 Kit
EUR 1132
  • Category: Cas9
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV120PA-KIT 1 Kit
EUR 1132
  • Category: Cas9
MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV125PA-KIT 1 Kit
EUR 1132
  • Category: Cas9
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS700A-KIT 10 rxn
EUR 1132
  • Category: Cas9
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS720A-KIT 10 rxn
EUR 1132
  • Category: Cas9
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS740A-KIT 10 rxn
EUR 1132
  • Category: Cas9
Complete Bacterial Protein Extraction Reagent
AR0157 50mL (sufficient for lysis of 10g of bacterial biomass)
EUR 141
Bacterial Total Protein Extraction Reagent
abx090632-50100assays 50-100 assays
EUR 258
  • Shipped within 5-10 working days.
EZLys? Bacterial Protein Extraction Reagent
8001-100
EUR 131
EZLys? Bacterial Protein Extraction Reagent
8001-500
EUR 294
Oxalate Decarboxylase, Active Bacterial Recombinant
7262-100
EUR 446
Oxalate Decarboxylase, Active Bacterial Recombinant
7262-1000
EUR 2931
Oxalate Decarboxylase, Active Bacterial Recombinant
7262-20
EUR 245
Sushi Peptide S3, bacterial recombinant
7611-100
EUR 370
Sushi Peptide S3, bacterial recombinant
7611-1000
EUR 1023
Sushi Peptide S3, bacterial recombinant
7611-20
EUR 153
Acetobacter xylinum Standard bacterial Strains
S0090 100 ul
EUR 365
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
CAS510A-KIT 1 Kit
EUR 805
  • Category: Cas9
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV200PA-KIT 1 Kit
EUR 1132
  • Category: Cas9
Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV205PA-KIT 1 Kit
EUR 1132
  • Category: Cas9
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV220PA-KIT 1 Kit
EUR 1132
  • Category: Cas9
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV225PA-KIT 1 Kit
EUR 1132
  • Category: Cas9
Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS750A-KIT 10 rxn
EUR 1132
  • Category: Cas9
Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS770A-KIT 10 rxn
EUR 1132
  • Category: Cas9
Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS790A-KIT 10 rxn
EUR 1132
  • Category: Cas9
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]
CAS9LIG-KIT 1 Kit
EUR 153
  • Category: Cas9
PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN320A-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools
PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN340iPS-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools
Bacterial Viability and Gram Stain Kit - 200 assays
32001 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE620A-KIT 1 kit
EUR 2132
  • Category: Gene Editing
5X Bacterial Protein Extraction Reagent (Tris)
AKR-180 50mL
EUR 276
Description: The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption.
5X Bacterial Protein Extraction Reagent (Phosphate)
AKR-181 50mL
EUR 276
Description: The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption.
Bacterial Sortase Substrate III, Abz/DNP
HY-P1883 5mg
EUR 652
Bacterial Outer Membrane Protein-A Protein
20-abx263222
  • EUR 481.00
  • EUR 1790.00
  • EUR 578.00
  • 100 ug
  • 1 mg
  • 200 ug
  • Shipped within 5-10 working days.
Recombinant Bacterial Outer Membrane Protein-A
7-05761 100µg Ask for price
Recombinant Bacterial Outer Membrane Protein-A
7-05762 200µg Ask for price
Recombinant Bacterial Outer Membrane Protein-A
7-05763 1mg Ask for price
CMCC (B) 51572 Standard bacterial Strains
S0081 100 ul
EUR 365
CMCC (B) 26003 Standard bacterial Strains
S0083 100 ul
EUR 365
CMCC (F) 98001 Standard bacterial Strains
S0084 100 ul
EUR 365
CMCC (B) 54002 Standard bacterial Strains
S0085 100 ul
EUR 365
CMCC (F) 98003 Standard bacterial Strains
S0088 100 ul
EUR 365
CMCC (B) 44102 Standard bacterial Strains
S0096 100 ul
EUR 365
CMCC (B) 10104 Standard bacterial Strains
S0097 100 ul
EUR 365
pC001 - huLshC2C2- MBP for bacterial expression
PVT10645 2 ug
EUR 301
AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE622A-KIT 1 kit
EUR 2132
  • Category: Gene Editing
AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE624A-KIT 1 kit
EUR 2132
  • Category: Gene Editing
Bacterial Lipopolysaccharides (LPS) Extraction Kit (up to 50 samples)
1000-100-LPS 1 kit
EUR 408
96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS585 5XPlates, 480prep
EUR 487.82
  • Product category: Molecular Biology Kits/RNA - Extraction/Bacteria
96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS5852 2XPlates, 192prep
EUR 250.31
  • Product category: Molecular Biology Kits/RNA - Extraction/Bacteria
vWF Acty. Kit
ABP-ACT-KIT 12 x 8 microwells
EUR 428
vWF Ant. Kit
ABP-TOT-KIT 12 x 8 microwells
EUR 394
hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)
CAS620A-KIT 1 kit
EUR 2152
  • Category: Cas9
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)
PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN300A-KIT 1 Kit
EUR 2798
  • Category: PinPoint Integrase Tools
PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN400A-KIT 1 Kit
EUR 2798
  • Category: PinPoint Integrase Tools
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN410A-KIT 1 Kit
EUR 4335
  • Category: PinPoint Integrase Tools
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN412A-KIT 1 Kit
EUR 4335
  • Category: PinPoint Integrase Tools
Bacterial/Permeability-Increasing Protein (BPI), Human Neutrophil
BPI15-N-50 50 ug
EUR 225
Bacterial Sortase Substrate III, Abz/DNP (TFA)
HY-P1883A 1mg
EUR 250
Superoxide Dismutase A (SOD-A), bacterial recombinant
6361-100
EUR 457
Helicobacter pylori Bacterial non-heme ferritin (ftnA)
1-CSB-YP009053HUV
  • EUR 679.00
  • EUR 335.00
  • EUR 2172.00
  • EUR 1051.00
  • EUR 1442.00
  • EUR 435.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 21.8 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Helicobacter pylori Bacterial non-heme ferritin(ftnA) expressed in Yeast
Helicobacter pylori Bacterial non-heme ferritin (ftnA)
1-CSB-EP009053HUV
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 24.8 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Helicobacter pylori Bacterial non-heme ferritin(ftnA) expressed in E.coli
Historically, the manipulation of DNA methylation demonstrates an unspecific function, adversely inflicting global-genome epigenetic alterations and complicated the operate function of desired gene on epigenetic regulation. Novel applied sciences for focused DNA methylation regulation have an ideal benefit of manipulating gene epigenetic alterations in a extra particular and environment friendly technique. On this evaluation, we described completely different site-specific concentrating on DNA methylation methods, together with each their benefits and limitations. By a complete understanding of those concentrating on instruments, we hope to open a brand new perspective for most cancers remedy.

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