Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Dec 10, 2020 Assay Kits

Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Background and goal: Flinders Expertise Associates (FTA) playing cards simplify pattern storage, transport, and extraction by decreasing price and time for analysis. This examine evaluated the FTA suitability for protected transport and storage of Gram-positive and Gram-negative bacterial cells of animal origin on its liquid tradition type and from organ impression smears (tissues) underneath the identical routine situation of microbiological laboratory together with detecting their nucleic acid over completely different storage circumstances.
Supplies and strategies: Enhance in bacterial depend from 104 to 107 (colony-forming items/mL) of 78 isolates representing seven bacterial species was utilized onto playing cards. FTA playing cards had been grouped and inoculated by these micro organism after which saved at completely different circumstances of 24-27°C, 4°C, and -20°C for 24 h, for two weeks, for 1 and three month storage, respectively. Bacteriological examination was completed, after which bacterial DNA was recognized utilizing particular primers for every bacterial kind and detected by polymerase chain response (PCR).
Outcomes: The whole share of recovered micro organism from FTA playing cards was 66.7% at 24-27°C for 24 h, the detection restrict was 100% in Gram-positive species, whereas it was 57.4% in Gram-negative ones. Relating to viable cell detection from organ impression smears, it was profitable underneath the earlier circumstances. No reside bacterial cells had been noticed by bacteriological isolation quite than solely at 24-27°C for 24 h storage. All bacterial DNA had been sufficiently confirmed by the PCR approach at completely different circumstances.
Conclusion: General, the FTA card technique was noticed to be a legitimate software for nucleic acid purification for micro organism of animal origin within the type of tradition or organ smears no matter its Gram kind and is used for a short while solely 24 h for storage and transport of reside micro organism particularly Gram-positive kind. Furthermore, the bacterial nucleic acid was intact after storage in -20°C for three months and was PCR amplifiable.

An environment friendly technique for the development of synthetic, concatemeric DNA, RNA and proteins with genetically programmed features, utilizing a novel, vector-enzymatic DNA fragment amplification-expression know-how

De novo designed bioactive molecules, equivalent to DNA, RNA and peptides, are utilized in more and more various scientific, industrial and biomedical functions. Concatemerization of designed DNA, RNA and peptides might enhance their stability, bioactivity and permit for gradual launch of the bioactive molecule on the supposed vacation spot. On this context, we developed a brand new technique enabling the formation of DNA concatemers for the manufacturing of synthetic, repetitive genes, encoding concatemeric RNAs and proteins of any nucleotide and amino-acid sequence.
The know-how recruits the Sort IIS SapI restriction endonuclease (REase) for assembling DNA fragments in an ordered head-to-tail-orientation. Alternatively, different commercially out there SapI isoschizomers can be utilized: LguI and thermostable BspQI. 4 sequence of DNA vectors devoted to the expression of newly shaped, concatemeric open studying frames (ORFs), had been designed and constructed to satisfy the know-how wants. • Vector-enzymatic DNA fragment amplification know-how. • Building of DNA concatemers many occasions longer than these out there with the usage of present de novo gene synthesis strategies. • Biosynthesis of protein tandem repeats with programmable operate by no means seen in nature. With the fast improvement of isothermal amplification know-how, DNA molecular analysis has grow to be an necessary reference for scientific remedy.
On this work, we’ve designed a DNA molecular diagnostic know-how with LAMP-like sensitivity for nucleic acid evaluation and detection based mostly on just one pair of hairpin primers. This DNA molecular diagnostic know-how consists of Bst DNA polymerase and one pair of hairpin primers, that are designed simply by including a stem-loop construction to a goal binding area. When the goal is current, the polymerization response between the hairpin primers and the goal generates a particular dumbbell DNA much like LAMP, which triggers cyclic amplification reactions to increase a sequence of lengthy dsDNA merchandise with repeated sequences by inserting fluorescent dye Eva Inexperienced noticed the rise in fluorescence sign.
Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions
Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Applied sciences for focused DNA methylation modifications in most cancers:Mechanism and utility

DNA methylation abnormalities are thought to be important occasion for most cancers initiation and improvement. Tumor-associated genes encompassing aberrant DNA methylation alterations at particular locus are correlated with gene silencing and chromatin reworking in varied malignancies. Thus, applied sciences designed to govern DNA methylation at particular loci of genome are crucial for the useful examine and therapeutic utility within the context of most cancers administration.

Milk Bacterial DNA Extraction Kit (50prep)

FAMBD-001 50 preps
EUR 328.8

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 84
Description: Bioscience Mol Bio; DNA Ladder

Bacterial DNA Extraction Kit (Proteinase K included)

BA05 5 preps Ask for price

Bacterial DNA Extraction Kit (Proteinase K included)

BA100 100 preps
EUR 165.6

Bacterial DNA Extraction Kit (Proteinase K included)

BA50 50 preps
EUR 120

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS423 50Preps
EUR 98.36

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS624 250preps
EUR 231.86

Bacterial Flagellin

VAdv-Ly0027 50 µg
EUR 9391.2
Description: Bacterial flagellin, a TLR5 agonist vaccine adjuvant.

Bacterial Collagenase Assay Kit

3014 1 kit
EUR 700.32
Description: Bacterial Collagenase Assay Kit

EasyRNA? Bacterial RNA Kit

K1351-250
EUR 1161.6

EasyRNA? Bacterial RNA Kit

K1351-50
EUR 444

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 186

Bacterial Counting Colorimetric Assay Kit

K511-2500
EUR 744

Bacterial Counting Colorimetric Assay Kit

K511-500
EUR 418.8

Ricketssia like bacterial PCR kit

PCR-V283-48D 50T
EUR 543.6

Ricketssia like bacterial PCR kit

PCR-V283-96D 100T
EUR 686.4

Bacterial Recombinant MDH

6357-100
EUR 574.8

Bacterial Recombinant GOR

6380-100
EUR 548.4

Bacterial Recombinant SurA

6381-100
EUR 548.4

Collagenase (bacterial) antibody

20C-CR6033RP 500 ul
EUR 516
Description: Rabbit polyclonal Collagenase (bacterial) antibody

NFNB, bacterial recombinant

7830-100
EUR 483.6

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Human Bacterial vaginosis (BV) ELISA Kit

abx052798-96tests 96 tests
EUR 801.6

Human bacterial vaginosis,BV ELISA Kit

201-12-1087 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human bacterial vaginosis(BV)ELISA Kit

GA-E1103HM-48T 48T
EUR 346.8

Human bacterial vaginosis(BV)ELISA Kit

GA-E1103HM-96T 96T
EUR 559.2

Human bacterial vaginosis(BV)ELISA Kit

QY-E00922 96T
EUR 433.2

Ricketssia like bacterial RT PCR kit

RTq-V283-100D 100T
EUR 860.4

Ricketssia like bacterial RT PCR kit

RTq-V283-150D 150T
EUR 969.6

Ricketssia like bacterial RT PCR kit

RTq-V283-50D 50T
EUR 717.6

Column Packing Kit

PACK-KIT 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

ATCC25922 Standard bacterial Strains

S0077 100 ul
EUR 438

ATCC25923 Standard bacterial Strains

S0078 100 ul
EUR 438

ATCC17802 Standard bacterial Strains

S0079 100 ul
EUR 438

ATCC16404 Standard bacterial Strains

S0080 100 ul
EUR 438

ATCC13813 Standard bacterial Strains

S0092 100 ul
EUR 438

ATCC27853 Standard bacterial Strains

S0110 100 ul
EUR 438

ATCC10792 Standard bacterial Strains

S0111 100 ul
EUR 438

ATCC19606 Standard bacterial Strains

S0113 100 ul
EUR 438

Agar Bacterial Tech 2Kg

281210 EACH
EUR 571.14

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

Live Bacterial Gram Stain Kit - 200 assays

32000-1 1kit
EUR 228
Description: Minimum order quantity: 1 unit of 1kit

Cell Meter™ Bacterial Viability Assay Kit

22400 200 Tests
EUR 367.2

3-min Bacterial Total Protein Extraction Kit

P512L - Ask for price

Ricketssia like bacterial One-Step PCR kit

Oneq-V283-100D 100T
EUR 1039.2

Ricketssia like bacterial One-Step PCR kit

Oneq-V283-150D 150T
EUR 1177.2

Ricketssia like bacterial One-Step PCR kit

Oneq-V283-50D 50T
EUR 861.6

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1358.4

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1358.4

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1358.4

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 966

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1358.4

Oxalate Decarboxylase, Active Bacterial Recombinant

7262-100
EUR 535.2

Oxalate Decarboxylase, Active Bacterial Recombinant

7262-1000
EUR 3517.2

Oxalate Decarboxylase, Active Bacterial Recombinant

7262-20
EUR 294

Bacterial Total Protein Extraction Reagent

abx090632-50100assays 50-100 assays
EUR 309.6

EZLys? Bacterial Protein Extraction Reagent

8001-100
EUR 157.2

EZLys? Bacterial Protein Extraction Reagent

8001-500
EUR 352.8

Sushi Peptide S3, bacterial recombinant

7611-100
EUR 444

Sushi Peptide S3, bacterial recombinant

7611-1000
EUR 1227.6

Sushi Peptide S3, bacterial recombinant

7611-20
EUR 183.6

Complete Bacterial Protein Extraction Reagent

AR0157 50mL (sufficient for lysis of 10g of bacterial biomass)
EUR 169.2

Acetobacter xylinum Standard bacterial Strains

S0090 100 ul
EUR 438

Suez Filter Cartridge Bacterial 0.1um

WAT2274 EACH
EUR 723.9

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1358.4

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 183.6

Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXMT-KIT 30ML
EUR 237.12

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 5929.2

Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXHM-KIT 30ML
EUR 245.1

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 5929.2

Bacterial Viability and Gram Stain Kit - 200 assays

32001 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

MycoLight™ Fluorescence Live/Dead Bacterial Imaging Kit

22411 100 Tests
EUR 446

MycoLight™ Rapid Fluorescence Bacterial Gram Stain Kit

22413 100 Tests
EUR 446

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2558.4

5X Bacterial Protein Extraction Reagent (Tris)

AKR-180 50mL
EUR 331.2
Description: The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption.

5X Bacterial Protein Extraction Reagent (Phosphate)

AKR-181 50mL
EUR 331.2
Description: The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption.

Recombinant Bacterial Outer Membrane Protein-A

7-05761 100µg Ask for price

Recombinant Bacterial Outer Membrane Protein-A

7-05762 200µg Ask for price

Recombinant Bacterial Outer Membrane Protein-A

7-05763 1mg Ask for price

Bacterial Outer Membrane Protein-A Protein

20-abx263222
  • EUR 577.20
  • EUR 2148.00
  • EUR 693.60
  • 100 ug
  • 1 mg
  • 200 ug

Bacterial Sortase Substrate III, Abz/DNP

HY-P1883 5mg
EUR 782.4

pC001 - huLshC2C2- MBP for bacterial expression

PVT10645 2 ug
EUR 361.2

CMCC (B) 51572 Standard bacterial Strains

S0081 100 ul
EUR 438

CMCC (B) 26003 Standard bacterial Strains

S0083 100 ul
EUR 438

CMCC (F) 98001 Standard bacterial Strains

S0084 100 ul
EUR 438

CMCC (B) 54002 Standard bacterial Strains

S0085 100 ul
EUR 438

CMCC (F) 98003 Standard bacterial Strains

S0088 100 ul
EUR 438

CMCC (B) 44102 Standard bacterial Strains

S0096 100 ul
EUR 438

CMCC (B) 10104 Standard bacterial Strains

S0097 100 ul
EUR 438

vWF Acty. Kit

ABP-ACT-KIT 12 x 8 microwells
EUR 513.6

vWF Ant. Kit

ABP-TOT-KIT 12 x 8 microwells
EUR 472.8

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS585 5XPlates, 480prep
EUR 585.38

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS5852 2XPlates, 192prep
EUR 300.37

Bacterial Lipopolysaccharides (LPS) Extraction Kit (up to 50 samples)

1000-100-LPS 1 kit
EUR 489.6

Bacterial RNA Polymerase Pyrophosphate Assay Kit (enzyme not included)

RPAP100K 100 assays
EUR 278.4
Description: This product includes 400 ul of Buffer RP, 33 ul of 100 x DNA, 33 ul of 100 x NTP mix, 5 ul of 1000 x pyrophosphatase and 4500 ul of dye for 100 assays of E. coli RNA polymerase reactions in a 384-well assay format. The assay This product includes all reagents except the enzyme.

PrecisionX Multiplex gRNA Cloning Kit

CAS9-GRNA-KIT 10 rxn
EUR 534

hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)

CAS620A-KIT 1 kit
EUR 2582.4
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN300A-KIT 1 Kit
EUR 3357.6

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN400A-KIT 1 Kit
EUR 3357.6

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN410A-KIT 1 Kit
EUR 5202
Historically, the manipulation of DNA methylation demonstrates an unspecific function, adversely inflicting global-genome epigenetic alterations and complicated the operate function of desired gene on epigenetic regulation. Novel applied sciences for focused DNA methylation regulation have an ideal benefit of manipulating gene epigenetic alterations in a extra particular and environment friendly technique. On this evaluation, we described completely different site-specific concentrating on DNA methylation methods, together with each their benefits and limitations. By a complete understanding of those concentrating on instruments, we hope to open a brand new perspective for most cancers remedy.

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