Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Dec 10, 2020 Assay Kits

Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Background and goal: Flinders Expertise Associates (FTA) playing cards simplify pattern storage, transport, and extraction by decreasing price and time for analysis. This examine evaluated the FTA suitability for protected transport and storage of Gram-positive and Gram-negative bacterial cells of animal origin on its liquid tradition type and from organ impression smears (tissues) underneath the identical routine situation of microbiological laboratory together with detecting their nucleic acid over completely different storage circumstances.
Supplies and strategies: Enhance in bacterial depend from 104 to 107 (colony-forming items/mL) of 78 isolates representing seven bacterial species was utilized onto playing cards. FTA playing cards had been grouped and inoculated by these micro organism after which saved at completely different circumstances of 24-27°C, 4°C, and -20°C for 24 h, for two weeks, for 1 and three month storage, respectively. Bacteriological examination was completed, after which bacterial DNA was recognized utilizing particular primers for every bacterial kind and detected by polymerase chain response (PCR).
Outcomes: The whole share of recovered micro organism from FTA playing cards was 66.7% at 24-27°C for 24 h, the detection restrict was 100% in Gram-positive species, whereas it was 57.4% in Gram-negative ones. Relating to viable cell detection from organ impression smears, it was profitable underneath the earlier circumstances. No reside bacterial cells had been noticed by bacteriological isolation quite than solely at 24-27°C for 24 h storage. All bacterial DNA had been sufficiently confirmed by the PCR approach at completely different circumstances.
Conclusion: General, the FTA card technique was noticed to be a legitimate software for nucleic acid purification for micro organism of animal origin within the type of tradition or organ smears no matter its Gram kind and is used for a short while solely 24 h for storage and transport of reside micro organism particularly Gram-positive kind. Furthermore, the bacterial nucleic acid was intact after storage in -20°C for three months and was PCR amplifiable.

An environment friendly technique for the development of synthetic, concatemeric DNA, RNA and proteins with genetically programmed features, utilizing a novel, vector-enzymatic DNA fragment amplification-expression know-how

De novo designed bioactive molecules, equivalent to DNA, RNA and peptides, are utilized in more and more various scientific, industrial and biomedical functions. Concatemerization of designed DNA, RNA and peptides might enhance their stability, bioactivity and permit for gradual launch of the bioactive molecule on the supposed vacation spot. On this context, we developed a brand new technique enabling the formation of DNA concatemers for the manufacturing of synthetic, repetitive genes, encoding concatemeric RNAs and proteins of any nucleotide and amino-acid sequence.
The know-how recruits the Sort IIS SapI restriction endonuclease (REase) for assembling DNA fragments in an ordered head-to-tail-orientation. Alternatively, different commercially out there SapI isoschizomers can be utilized: LguI and thermostable BspQI. 4 sequence of DNA vectors devoted to the expression of newly shaped, concatemeric open studying frames (ORFs), had been designed and constructed to satisfy the know-how wants. • Vector-enzymatic DNA fragment amplification know-how. • Building of DNA concatemers many occasions longer than these out there with the usage of present de novo gene synthesis strategies. • Biosynthesis of protein tandem repeats with programmable operate by no means seen in nature. With the fast improvement of isothermal amplification know-how, DNA molecular analysis has grow to be an necessary reference for scientific remedy.
On this work, we’ve designed a DNA molecular diagnostic know-how with LAMP-like sensitivity for nucleic acid evaluation and detection based mostly on just one pair of hairpin primers. This DNA molecular diagnostic know-how consists of Bst DNA polymerase and one pair of hairpin primers, that are designed simply by including a stem-loop construction to a goal binding area. When the goal is current, the polymerization response between the hairpin primers and the goal generates a particular dumbbell DNA much like LAMP, which triggers cyclic amplification reactions to increase a sequence of lengthy dsDNA merchandise with repeated sequences by inserting fluorescent dye Eva Inexperienced noticed the rise in fluorescence sign.
Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions
Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

Applied sciences for focused DNA methylation modifications in most cancers:Mechanism and utility

DNA methylation abnormalities are thought to be important occasion for most cancers initiation and improvement. Tumor-associated genes encompassing aberrant DNA methylation alterations at particular locus are correlated with gene silencing and chromatin reworking in varied malignancies. Thus, applied sciences designed to govern DNA methylation at particular loci of genome are crucial for the useful examine and therapeutic utility within the context of most cancers administration.

Bacterial DNA Extraction Kit

MBS8808212-50Tests 50Tests
EUR 280

Bacterial DNA Purification Kit II

K1457-250 250 Preps
EUR 697.2

Bacterial DNA Purification Kit II

K1457-50 50 Preps
EUR 381.6

EcoSpin Bacterial Genomic DNA Kit

E1050 50 rxn
EUR 53.9
Description: EcoSpin Bacterial Genomic DNA Kit is designed as a simple and convenient purification of high quality genomic DNA from Gram (-) negative and Gram (+) positive bacterial cells. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 25 minutes and purified DNA can be used directly in PCR, qPCR, sequencing and enzymatic reactions.

Bacterial Genomic DNA Isolation Kit

K309-100 each
EUR 451.2

Milk Bacterial DNA Extraction Kit (50prep)

FAMBD-001 50 preps
EUR 328.8

Milk Bacterial DNA Extraction Kit (50prep)

FAMBD_001 1 kit
EUR 134.5

GeneMATRIX TISSUE & BACTERIAL DNA Purification Kit

E3551-01 50preps
EUR 98.1
Description: Kit for isolation of total DNA from human and animal tissues and bacteria

GeneMATRIX TISSUE & BACTERIAL DNA Purification Kit

E3551-02 150preps
EUR 275.77
Description: Kit for isolation of total DNA from human and animal tissues and bacteria

Bacterial DNA Extraction Kit (Proteinase K included)

BA50 50 preps
EUR 120

Bacterial DNA Extraction Kit (Proteinase K included)

BA05 5 preps Ask for price

Bacterial DNA Extraction Kit (Proteinase K included)

BA100 100 preps
EUR 165.6

Bacterial DNA Extraction Kit (Proteinase K included)

BA96-05 5x96 preps
EUR 694

AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria

Z1322006 1 kit (48 extractions) Including all required plastic disposables
EUR 259

Column-Pure Bacterial Genomic DNA Isolation Kit

D511 50 Preparations
EUR 195

SaMag Bacterial DNA Extraction kit For use with SaMag-12/24 instruments; extraction of bacterial DNA of from clinical specimens or coltures

SM006 48
EUR 187.48

GeneMATRIX BACTERIAL & YEAST GENOMIC DNA Purification Kit

E3580-01 50preps
EUR 95.92

GeneMATRIX BACTERIAL & YEAST GENOMIC DNA Purification Kit

E3580-02 150preps
EUR 270.32

HiPer® Bacterial Genomic DNA Extraction

HTBM008E-10PR 1 unit
EUR 32.63
Description: HiPer® Bacterial Genomic DNA Extraction

HiPer® Bacterial Genomic DNA Extraction

HTBM009E-10PR 1 unit
EUR 37.4
Description: HiPer® Bacterial Genomic DNA Extraction

Insta NX® Bacterial Genomic DNA Purifica

MBIN006-12PR 1 unit
EUR 71.73
Description: Insta NX® Bacterial Genomic DNA Purifica

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS423 50Preps
EUR 98.36

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS624 250preps
EUR 231.86

HiPurA® Bacterial Genomic DNA Miniprep P

MB505-20PR 1 unit
EUR 38.72
Description: HiPurA® Bacterial Genomic DNA Miniprep P

HiPurA® Bacterial Genomic DNA Miniprep P

MB505-250PR 1 unit
EUR 415.6
Description: HiPurA® Bacterial Genomic DNA Miniprep P

HiPurA® Bacterial Genomic DNA Miniprep P

MB505-50PR 1 unit
EUR 91.51
Description: HiPurA® Bacterial Genomic DNA Miniprep P

ExoQuali™ Bacterial MVs Isolation Kit (bacterial culture media)

CEIK-092L-10T 10 T Ask for price

ExoQuali™ Bacterial MVs Isolation Kit (bacterial culture media)

CEIK-092L-20T 20 T Ask for price

HiPurA® 96-Bacterial Genomic DNA Purific

MB548-1X96PR 1 unit
EUR 172.52
Description: HiPurA® 96-Bacterial Genomic DNA Purific

GF-1 Bacterial Starter Kit / Taq DNA Polymerase, 25 preps

GF-BA-K each Ask for price

GF-1 Bacterial DNA Extraction Kit (Proteinase K included), 50 preps

GF-BA-050 each Ask for price

GF-1 Bacterial DNA Extraction Kit (Proteinase K included), 100 preps

GF-BA-100 each Ask for price

Bacterial Transformation Kit

MBT127-10R 1 unit
EUR 18.24
Description: Bacterial Transformation Kit

Bacterial Transformation Kit

MBT127-20R 1 unit
EUR 32.87
Description: Bacterial Transformation Kit

Bacterial Transformation Kit

MBT127-40R 1 unit
EUR 58.64
Description: Bacterial Transformation Kit

EasyRNA? Bacterial RNA Kit

K1351-250 each
EUR 1161.6

EasyRNA? Bacterial RNA Kit

K1351-50 each
EUR 444

GF-1 Bacterial Starter Kit / Chromo Taq DNA Polymerase, 25 preps

GF-BA-KW each Ask for price

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 186

Bacterial Collagenase Assay Kit

3014 1 kit
EUR 409
Description: Culture Media, Tissue Homogenate

Bacterial Flagellin

VAdv-Ly0027 50 µg
EUR 9391.2
Description: Bacterial flagellin, a TLR5 agonist vaccine adjuvant.

genesigPLEX GI Bacterial Kit 2

R01046 100 tests
EUR 1777

genesigPLEX Bacterial Meningitis Kit

R01002 100 tests
EUR 1777

Ricketssia like bacterial PCR kit

PCR-V283-48D 50T
EUR 543.6

Ricketssia like bacterial PCR kit

PCR-V283-96D 100T
EUR 686.4

Live-Dead Bacterial Staining Kit

K2239-100 100T
EUR 300.8
Description: Cell Biology|Cell Proliferation & Toxicity

Live-Dead Bacterial Staining Kit

K2239-20 20T
EUR 78.4
Description: Cell Biology|Cell Proliferation & Toxicity

genesigPLEX Gastrointestinal Bacterial Kit

R01004 100 tests
EUR 1777

Bacterial Recombinant MDH

6357-100 each
EUR 574.8

Bacterial Recombinant GOR

6380-100 each
EUR 548.4

Ricketssia like bacterial

Oneq-V283-OneqV283100D Oneq-V283-100D
EUR 515

Ricketssia like bacterial

Oneq-V283-OneqV283150D Oneq-V283-150D
EUR 594

Ricketssia like bacterial

Oneq-V283-OneqV28350D Oneq-V283-50D
EUR 413

Ricketssia like bacterial

PCR-V283-PCRV28348D PCR-V283-48D
EUR 230

Ricketssia like bacterial

PCR-V283-PCRV28396D PCR-V283-96D
EUR 312

Ricketssia like bacterial RT PCR kit

RTq-V283-100D 100T
EUR 860.4

Ricketssia like bacterial RT PCR kit

RTq-V283-150D 150T
EUR 969.6

Ricketssia like bacterial RT PCR kit

RTq-V283-50D 50T
EUR 717.6

MycoLightâ„¢ Bacterial Viability Assay Kit

22400-200Tests 200 Tests
EUR 334
Description: AAT Bioquest's Mycolight™ Bacterial Viability Assay Kit provides two-color fluorescence assay of bacterial viability in both gram-positive and negative bacterial cell.

Bacterial Transformation Kit - 1 (Teaching)

64279 15 expt. Kit
EUR 78.77
Description: Part E

Bacterial Transformation Kit - 1 (Teaching)

64279-1 30 expt. Kit
EUR 111.53
Description: Part E

NFNB, bacterial recombinant

7830-100 each
EUR 483.6

Bacterial Recombinant SurA

6381-100 each
EUR 548.4

Bacterial Counting Colorimetric Assay Kit

K511-2500 each
EUR 744

Bacterial Counting Colorimetric Assay Kit

K511-500 each
EUR 418.8

Human Bacterial vaginosis (BV) ELISA Kit

abx052798-96tests 96 tests
EUR 801.6

Human bacterial vaginosis,BV ELISA Kit

YLA2512HU-48T 48T Ask for price

Human bacterial vaginosis,BV ELISA Kit

YLA2512HU-96T 96T Ask for price

Human bacterial vaginosis(BV)ELISA Kit

GA-E1103HM-48Tests 48 Tests Ask for price

Human bacterial vaginosis(BV)ELISA Kit

GA-E1103HM-96Tests 96 Tests
EUR 388

Human bacterial vaginosis(BV)ELISA Kit

GA-E1103HM-48T 48T
EUR 346.8

Human bacterial vaginosis(BV)ELISA Kit

GA-E1103HM-96T 96T
EUR 559.2

Human bacterial vaginosis(BV)ELISA Kit

QY-E00922 96T
EUR 433.2

Human bacterial vaginosis,BV ELISA Kit

SL0328Hu 96 Tests
EUR 468

Human bacterial vaginosis (BV) ELISA Kit

MBS9311237-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human bacterial vaginosis (BV) ELISA Kit

MBS9311237-48StripWells 48-Strip-Wells
EUR 550

Human bacterial vaginosis (BV) ELISA Kit

MBS9311237-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human bacterial vaginosis (BV) ELISA Kit

MBS9311237-96StripWells 96-Strip-Wells
EUR 765

Human bacterial vaginosis, BV ELISA Kit

MBS9302678-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human bacterial vaginosis, BV ELISA Kit

MBS9302678-48StripWells 48-Strip-Wells
EUR 550

Human bacterial vaginosis, BV ELISA Kit

MBS9302678-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human bacterial vaginosis, BV ELISA Kit

MBS9302678-96StripWells 96-Strip-Wells
EUR 765

Human bacterial vaginosis,BV ELISA Kit

201-12-1087 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Bacterial Sialidase Antibody

GWB-9CC345 1 ml Ask for price

HiPurA™ Bacterial RNA Purification Kit

MB613-20PR 1 unit
EUR 59.86
Description: HiPurA™ Bacterial RNA Purification Kit

HiPurA™ Bacterial RNA Purification Kit

MB613-250PR 1 unit
EUR 560.73
Description: HiPurA™ Bacterial RNA Purification Kit

HiPurA™ Bacterial RNA Purification Kit

MB613-50PR 1 unit
EUR 119.46
Description: HiPurA™ Bacterial RNA Purification Kit

Live Bacterial Gram Stain Kit - 800 assays

32000-1 1kit
EUR 393
Description: Lectin from wheat germ (Triticum aestivum)

Live Bacterial Gram Stain Kit - 200 assays

32000-1-1 KT
EUR 158

Collagenase (bacterial) antibody

20C-CR6033RP 500 ul
EUR 337
Description: Rabbit polyclonal Collagenase (bacterial) antibody

Collagenase (bacterial) antibody

MBS535696-05mL 0.5mL
EUR 725

Collagenase (bacterial) antibody

MBS535696-5x05mL 5x0.5mL
EUR 3105

AFTSpin Bacterial Fast RNA Extraction Kit

RK30123 50RXN
EUR 38.15

Bacterial Organophosphorus Medium

HY-157359 Get quote Ask for price
Description: Bacterial Organophosphorus Medium can be used to determine the efficiency of phosphorus bacteria in decomposing organic phosphorus in phosphorus bacteria fertilizers. Bacterial Organophosphorus Medium ingredients include glucose, ammonium sulfate, yeast extract, sodium chloride, potassium chloride, magnesium sulfate, ferrous sulfate, manganese sulfate, lecithin, calcium carbonate, agar[1].

HiPer® Bacterial RNA Teaching Kit (Colum

HTBM042-10PR 1 unit
EUR 32.63
Description: HiPer® Bacterial RNA Teaching Kit (Colum

Bacterial Endotoxin Test Water

E028 each
EUR 263.2

Bacterial Protein Extraction Kit with Lysozyme

18-402 100 Preps/Unit
EUR 193.81
Description: Total Protein Extraction

Bacterial Protein Extraction Kit with Lysozyme

18-403 200 Preps/Unit
EUR 372.94
Description: Total Protein Extraction

RABBIT ANTI BACTERIAL SIALIDASE

MBS221018-1mL 1mL
EUR 565

RABBIT ANTI BACTERIAL SIALIDASE

MBS221018-5x1mL 5x1mL
EUR 2360

Agar Bacterial Tech 2Kg - EACH

281210 EACH
EUR 772.2

Ricketssia like bacterial One-Step PCR kit

Oneq-V283-100D 100T
EUR 1039.2

Ricketssia like bacterial One-Step PCR kit

Oneq-V283-150D 150T
EUR 1177.2

Ricketssia like bacterial One-Step PCR kit

Oneq-V283-50D 50T
EUR 861.6

Bacterial Luciferase Antibody (HRP)

GWB-AE01B9 0.1 ml Ask for price

Bacterial Luciferase Antibody (HRP)

GWB-BE5D59 1 mg Ask for price

3-min Bacterial Total Protein Extraction Kit

P512L - Ask for price

Bacterial Luciferase Antibody (FITC)

GWB-5FC9AA 0.1 ml Ask for price

Bacterial Protein Extraction Buffer

18-400 250 mL/Unit
EUR 119.05
Description: Total Protein Extraction

Bacterial Protein Extraction Buffer

18-401 500 mL/Unit
EUR 226.89
Description: Total Protein Extraction

Bacterial Protein Extraction Buffer

20010 200 ml
EUR 130
Description: BPS Bacterial Protein extraction Buffer enables extraction of both soluble and insoluble (inclusion bodies) proteins from bacteria. The buffer provides high efficiency recovery of over-expressed proteins and is fully compatible with HIS/GST-tag purification procedures and standard protein concentration determination assays. The buffer can also be used for protein extraction from insect cells.

Bacterial Protein Extraction Buffer

20020 500 ml
EUR 245
Description: BPS Bacterial Protein extraction Buffer enables extraction of both soluble and insoluble (inclusion bodies) proteins from bacteria. The buffer provides high efficiency recovery of over-expressed proteins and is fully compatible with HIS/GST-tag purification procedures and standard protein concentration determination assays. The buffer can also be used for protein extraction from insect cells.

Bacterial Protein Extraction Buffer

GWB-PS6392 200mL Ask for price

Bacterial Protein Extraction Buffer

GWB-PSF9DC 500mL Ask for price
Historically, the manipulation of DNA methylation demonstrates an unspecific function, adversely inflicting global-genome epigenetic alterations and complicated the operate function of desired gene on epigenetic regulation. Novel applied sciences for focused DNA methylation regulation have an ideal benefit of manipulating gene epigenetic alterations in a extra particular and environment friendly technique. On this evaluation, we described completely different site-specific concentrating on DNA methylation methods, together with each their benefits and limitations. By a complete understanding of those concentrating on instruments, we hope to open a brand new perspective for most cancers remedy.

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