Best Commercial Plasmid DNA Kit

Genetici-technologies

Comparability of six business kits to extract bacterial chromosome and plasmid DNA for MiSeq sequencing.

We in contrast business kits for extraction of genomic DNA from the Gram-negative bacterium Klebsiella pneumoniae for subsequent Miseq sequencing.

 

Purification of DNA was based mostly on matrix binding (silica or anion alternate resin) or differential precipitation (salting out), respectively.

 

The selection of extraction equipment had little impact on sequencing high quality and protection throughout drastically completely different replicons, aside from an obvious depletion of small plasmids (<5 kb) throughout precipitation-based extractions.

 

Genomic DNA Isolation Kit | Soil Genomic DNA Kit

Description: Genomic DNA Isolation Kit for use in the research laboratory

 

Sequencing protection supplied copy-number estimates for small plasmids that have been persistently larger than these from quantitative real-time PCR.

Genetici-technologies
Genetici-technologies

Excessive effectivity technique to acquire supercoiled DNA with a business plasmid purification equipment.

Supercoiled state corresponds to the energetic type for plasmid purposes. The relaxed round type of plasmids is commonly inactive or poorly energetic.

 

To acquire vital quantities of virtually absolutely supercoiled DNA, we modified the usual protocol of a commercially accessible Qiagen plasmid purification equipment. Our modifications led to isolation of virtually 100% of the plasmids within the supercoiled state.

Fungal Genomic DNA Kit | Plant Genomic DNA Kit

The modified protocol was used to purify completely different plasmids with constant outcomes. The purified plasmids keep supercoiled state for about two months.

 

The modified protocol could be very advantageous as a result of it permits straightforward DNA manufacturing with excessive diploma of supercoiled type at low price.

 

GENOMIC DNA KIT

IB47251 4 X 96 PREP KIT
EUR 1464.04

GENOMIC DNA KIT

IB47252 10 X 96 PREP KIT
EUR 3254.61

Genomic DNA Kit

abx098076-100l 100 µl
EUR 300

Genomic DNA Kit

abx098076-1ml 1 ml Ask for price

Genomic DNA Kit

abx098076-200l 200 µl
EUR 550

Genomic DNA Mini Kit (Blood/Cultured Cell)

GB004 each
EUR 3

Genomic DNA Mini Kit (Blood/Cultured Cell)

GB100 each
EUR 70

Genomic DNA Mini Kit (Blood/Cultured Cell)

GB300 each
EUR 189

Genomic DNA Maxi Kit (Blood/Cultured Cell)

GDM002 each
EUR 21

Genomic DNA Maxi Kit (Blood/Cultured Cell)

GDM010 each
EUR 89.3

Genomic DNA Maxi Kit (Blood/Cultured Cell)

GDM025 each
EUR 199.5

Soil Genomic DNA Kit

K1411-250 each
EUR 673.2

Soil Genomic DNA Kit

K1411-50 each
EUR 379.2

Plant Genomic DNA Kit

20-abx098077
  • Ask for price
  • Ask for price
  • 200 rxns
  • 50 rxns

Micro Genomic DNA Kit

20-abx098242
  • Ask for price
  • Ask for price
  • 2 rxns
  • 50 rxns

Blood Genomic DNA Kit

abx098868-50rxns 50 rxns
EUR 326.4

Plant Genomic DNA Kit

abx294004-50preps 50 preps
EUR 477.6

Yeast Genomic DNA Kit

K1414-250 each
EUR 673.2

Yeast Genomic DNA Kit

K1414-50 each
EUR 379.2

Plant Genomic DNA Kit

abx098077-100l 100 µl
EUR 287.5

Plant Genomic DNA Kit

abx098077-1ml 1 ml Ask for price

Plant Genomic DNA Kit

abx098077-200l 200 µl
EUR 512.5

Micro Genomic DNA Kit

abx098242-100l 100 µl
EUR 812.5

Micro Genomic DNA Kit

abx098242-1ml 1 ml Ask for price

Micro Genomic DNA Kit

abx098242-200l 200 µl Ask for price

Blood Genomic DNA Kit

abx098868-100l 100 µl
EUR 337.5

Blood Genomic DNA Kit

abx098868-1ml 1 ml Ask for price

Blood Genomic DNA Kit

abx098868-200l 200 µl Ask for price

Plant Genomic DNA Kit

abx294004-100g 100 µg Ask for price

Plant Genomic DNA Kit

abx294004-20g 20 µg
EUR 312.5

Plant Genomic DNA Kit

abx294004-50g 50 µg Ask for price

Animal Genomic DNA Kit

abx294005-100preps 100 preps
EUR 627.6

Animal Genomic DNA Kit

abx294005-50preps 50 preps
EUR 477.6

Insect Genomic DNA Kit

K1412-250 each
EUR 673.2

Insect Genomic DNA Kit

K1412-50 each
EUR 379.2

Fungal Genomic DNA Kit

K1415-250 each
EUR 673.2

Fungal Genomic DNA Kit

K1415-50 each
EUR 379.2

Animal Genomic DNA Kit

abx294005-100g 100 µg Ask for price

Animal Genomic DNA Kit

abx294005-20g 20 µg
EUR 312.5

Animal Genomic DNA Kit

abx294005-50g 50 µg
EUR 725

Mollusc Genomic DNA Kit

K1413-250 each
EUR 673.2

Mollusc Genomic DNA Kit

K1413-50 each
EUR 379.2

Bacteria Genomic DNA Kit

20-abx098080
  • Ask for price
  • Ask for price
  • 2 rxns
  • 50 rxns

Bacteria Genomic DNA Kit

abx098080-100l 100 µl
EUR 300

Bacteria Genomic DNA Kit

abx098080-1ml 1 ml Ask for price

Bacteria Genomic DNA Kit

abx098080-200l 200 µl Ask for price

Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)

PDC09-0100 100 rxns
EUR 99

Genomic DNA - Human Tumor Cell Line: A431

D1255801 100 ug
EUR 282

Genomic DNA - Human Tumor Cell Line: Hela

D1255811 100 ug
EUR 282

Genomic DNA - Human Tumor Cell Line: Raji

D1255840 100 ug
EUR 282

Cell & tissue genomic DNA extraction and purification kit

K1442-100 each
EUR 679.2

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 273
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation Kit

GWB-AXR187 50 assays Ask for price

Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 389
Description: Isolates highly pure Genomic DNA within 90 min.

Genomic DNA Isolation Kit

K281-50 each
EUR 326.4

Genomic DNA Isolation Kit

abx460025-1096tests 10 × 96 tests Ask for price

Genomic DNA Isolation Kit

abx460025-596tests 5 × 96 tests Ask for price

Genomic DNA Isolation Kit

abx460025-96tests 96 tests
EUR 437.5

Genomic DNA - Human Tumor Cell Line: Jurkat

D1255815 100 ug
EUR 282

gBAC MINI GENOMIC DNA KIT

IB47290 4 PREP SAMPLE KIT
EUR 30.42

gBAC MINI GENOMIC DNA KIT

IB47291 100 PREP KIT
EUR 269.93

gBAC MINI GENOMIC DNA KIT

IB47292 300 PREP KIT
EUR 725.56

Insta NX® Cell Genomic DNA Purification

MBIN011-12PR 1 unit
EUR 71.73
Description: Insta NX® Cell Genomic DNA Purification

ISOLATE II Genomic DNA Kit

BIO-52065 10 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52066 50 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52067 250 preps Ask for price

gSWAB MINI GENOMIC DNA KIT

IB47275 4 PREP SAMPLE KIT
EUR 30.11

 

 

Zenoquick Genomic DNA Purification Kit

 

Plasmid DNA purification from E. coli is a core approach for molecular cloning. Small scale purification (miniprep) from lower than 5 ml of bacterial tradition is a fast means for clone verification or DNA isolation, adopted by additional enzymatic reactions (polymerase chain response and restriction enzyme digestion).

 

Right here, we video-recorded the final procedures of miniprep via the QIAGEN’s QIAprep eight Miniprep Equipment, aiming to introducing this extremely environment friendly approach to the final learners for molecular biology strategies.

 

The entire process relies on alkaline lysis of E. coli cells adopted by adsorption of DNA onto silica within the presence of excessive salt.

 

Cell & tissue genomic DNA extraction and purification kit

Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

It consists of three steps: 1) preparation and clearing of a bacterial lysate, 2) adsorption of DNA onto the QIAprep membrane, 3) washing and elution of plasmid DNA.

 

Genomic DNA from Plant Regular Tissue: Arabidopsis

Description: Can be utilized for numerous research within the realm of gene expression, each regular and pathological. It is a superb management and appropriate for academic functions.  Can be utilized for numerous research within the realm of gene expression, each regular and pathological. It is a superb management and appropriate for academic functions.

 

 

All steps are carried out with out the use of phenol, chloroform, CsCl, ethidium bromide, and with out alcohol precipitation. It normally takes lower than 2 hours to complete your complete process.

 

Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification equipment, for era of huge quantities of plasmid DNA.

The GenomiPhi DNA Amplification Equipment employs rolling circle amplification (RCA) utilizing phi29 polymerase, dNTPs, and random hexamers. We demonstrated that repeated RCA (a minimum of 3 times) is helpful for high-fidelity amplification of huge quantities of plasmid DNA.

 

Toxin Child uncouples DNA replication and cell division to implement retention of plasmid R1 in Escherichia coli cells.

Worldwide dissemination of antibiotic resistance in micro organism is facilitated by plasmids that encode postsegregational killing (PSK) techniques.

 

These produce a secure toxin (T) and a labile antitoxin (A) conditioning cell survival to plasmid upkeep, as a result of solely this ensures neutralization of toxicity.

 

Scarcity of antibiotic options and the hyperlink of TA pairs to PSK have stimulated the opinion that untimely toxin activation could possibly be used to kill these recalcitrant organisms within the clinic.

 

Nevertheless, validation of TA pairs as therapeutic targets requires unambiguous understanding of their mode of motion, penalties for cell viability, and performance in plasmids.

Rapid Blood Genomic DNA Extraction Kit

Conflicting with widespread notions regarding these points, we had proposed that the TA pair kis-kid (killing suppressor-killing determinant) would possibly operate as a plasmid rescue system and never as a PSK system, however this remained to be validated.

 

Right here, we aimed to make clear unsettled mechanistic features of Child activation, and of the results of this for kis-kid-bearing plasmids and their host cells.

Easy3-Plant Genomic DNA Isolation Kit

We verify that activation of Child happens in cells which might be about to lose the toxin-encoding plasmid, and we present that this provokes extremely selective restriction of protein outputs that inhibits cell division briefly, avoiding plasmid loss, and stimulates DNA replication, selling plasmid rescue.

 

Kis and Child are conserved in plasmids encoding a number of antibiotic resistance genes, together with prolonged spectrum β-lactamases, for which therapeutic choices are scarce, and our findings advise in opposition to the activation of this TA pair to combat pathogens carrying these extrachromosomal DNAs.

 

 

 

 

Genomic DNA Mini Kit (Tissue)

GT004 each
EUR 3

Genomic DNA Mini Kit (Tissue)

GT050 each
EUR 63.5

Genomic DNA Mini Kit (Tissue)

GT100 each
EUR 105

Genomic DNA Mini Kit (Tissue)

GT300 each
EUR 283.5

Genomic DNA Isolation Kit (Tissue)

PDC11-0100 100 rxns
EUR 132

Magnetic bead Tissue Genomic DNA Kit

PDM02-0100 100 rcs
EUR 265

Dual Genomic DNA Isolation Kit(Tissue)

PDC06-0100 100 rxns
EUR 154

Plant Tissue Genomic DNA Isolation Kit

K316-100 each
EUR 451.2

Magnetic Beads-Based Tissue Genomic DNA Kit

abx460016-1096tests 10 × 96 tests Ask for price

Magnetic Beads-Based Tissue Genomic DNA Kit

abx460016-596tests 5 × 96 tests Ask for price

Magnetic Beads-Based Tissue Genomic DNA Kit

abx460016-96tests 96 tests
EUR 437.5

Genomic DNA Extraction KitAnimal Tissue

FYG102-100P 100 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-300P 300 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-50P 50 Preps Ask for price

Genomic DNA - Rat Normal Tissue: Lung

D1434152 100 ug
EUR 329

Genomic DNA - Rat Normal Tissue: Brain

D1434035 100 ug
EUR 329

Genomic DNA - Rat Normal Tissue: Heart

D1434122 100 ug
EUR 329

Genomic DNA - Rat Normal Tissue: Liver

D1434149 100 ug
EUR 329

Genomic DNA - Mouse Normal Tissue: Lung

D1334152 100 ug
EUR 329

Genomic DNA - Rat Normal Tissue: Kidney

D1434142 100 ug
EUR 329

Genomic DNA - Rat Normal Tissue: Testis

D1434260 100 ug
EUR 329

Genomic DNA - Rat Normal Tissue: Thymus

D1434264 100 ug
EUR 329

Genomic DNA - Plant Normal Tissue: Corn

D1634330 10 ug
EUR 244

Genomic DNA - Plant Normal Tissue: Rice

D1634360 10 ug
EUR 244

Genomic DNA - Mouse Normal Tissue: Brain

D1334035 100 ug
EUR 329

Genomic DNA - Mouse Normal Tissue: Heart

D1334122 100 ug
EUR 329

Genomic DNA - Mouse Normal Tissue: Liver

D1334149 100 ug
EUR 329

Genomic DNA - Rat Normal Tissue: Stomach

D1434248 100 ug
EUR 329

Genomic DNA - Plant Normal Tissue: Wheat

D1634390 10 ug
EUR 244

Genomic DNA Extraction Mini KitPlant Tissue

FYG103-100P 100 Preps Ask for price

Genomic DNA - Mouse Normal Tissue: Kidney

D1334142 100 ug
EUR 329

Genomic DNA - Mouse Normal Tissue: Spleen

D1334246 100 ug
EUR 329

Genomic DNA - Plant Normal Tissue: Orange

D1634340 10 ug
EUR 244

Genomic DNA - Plant Normal Tissue: Potato

D1634350 10 ug
EUR 244

Genomic DNA - Mouse Normal Tissue: Stomach

D1334248 100 ug
EUR 329

Genomic DNA Isolation Kit (Paraffin-embedded tissue)

PDC12-0100 100 rxns
EUR 132

Genomic DNA - Plant Normal Tissue: Arabidopsis

D1634310 10 ug
EUR 629