Best Commercial Plasmid DNA Kit

Genetici-technologies

Comparability of six business kits to extract bacterial chromosome and plasmid DNA for MiSeq sequencing.

We in contrast business kits for extraction of genomic DNA from the Gram-negative bacterium Klebsiella pneumoniae for subsequent Miseq sequencing.

 

Purification of DNA was based mostly on matrix binding (silica or anion alternate resin) or differential precipitation (salting out), respectively.

 

The selection of extraction equipment had little impact on sequencing high quality and protection throughout drastically completely different replicons, aside from an obvious depletion of small plasmids (<5 kb) throughout precipitation-based extractions.

 

Genomic DNA Isolation Kit | Soil Genomic DNA Kit

Description: Genomic DNA Isolation Kit for use in the research laboratory

 

Sequencing protection supplied copy-number estimates for small plasmids that have been persistently larger than these from quantitative real-time PCR.

Genetici-technologies
Genetici-technologies

Excessive effectivity technique to acquire supercoiled DNA with a business plasmid purification equipment.

Supercoiled state corresponds to the energetic type for plasmid purposes. The relaxed round type of plasmids is commonly inactive or poorly energetic.

 

To acquire vital quantities of virtually absolutely supercoiled DNA, we modified the usual protocol of a commercially accessible Qiagen plasmid purification equipment. Our modifications led to isolation of virtually 100% of the plasmids within the supercoiled state.

Fungal Genomic DNA Kit | Plant Genomic DNA Kit

The modified protocol was used to purify completely different plasmids with constant outcomes. The purified plasmids keep supercoiled state for about two months.

 

The modified protocol could be very advantageous as a result of it permits straightforward DNA manufacturing with excessive diploma of supercoiled type at low price.

 

Micro Genomic DNA Kit
20-abx098242
  • EUR 105.00
  • EUR 328.00
  • 2 rxns
  • 50 rxns

Blood Genomic DNA Kit
abx098868-50rxns 50 rxns
EUR 272

Plant Genomic DNA Kit
abx294004-50preps 50 preps
EUR 398

Animal Genomic DNA Kit
abx294005-100preps 100 preps
EUR 523

Animal Genomic DNA Kit
abx294005-50preps 50 preps
EUR 398

Genomic DNA Isolation Kit
K2118-50 50 assays
EUR 321

Genomic DNA Isolation Kit
55R-1362 50 assays
EUR 385
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA Extraction Kit
K5016005 1 kit
EUR 329

Soil Genomic DNA Kit
K1411-250
EUR 561

Soil Genomic DNA Kit
K1411-50
EUR 316

Insect Genomic DNA Kit
K1412-250
EUR 561

Insect Genomic DNA Kit
K1412-50
EUR 316

Mollusc Genomic DNA Kit
K1413-250
EUR 561

Mollusc Genomic DNA Kit
K1413-50
EUR 316

Yeast Genomic DNA Kit
K1414-250
EUR 561

Yeast Genomic DNA Kit
K1414-50
EUR 316

Fungal Genomic DNA Kit
K1415-250
EUR 561

Fungal Genomic DNA Kit
K1415-50
EUR 316

Genomic DNA Isolation Kit
K281-50
EUR 272

Human Genomic DNA
BIO-35025 500µl @ 200ng/µl Ask for price

Human Genomic DNA
X11000 0.2 ml Ask for price
Description: reagents widely cited

ISOLATE II Genomic DNA Kit
BIO-52065 10 preps Ask for price

ISOLATE II Genomic DNA Kit
BIO-52066 50 preps Ask for price

ISOLATE II Genomic DNA Kit
BIO-52067 250 preps Ask for price

Marine Animal Genomic DNA Kit
20-abx098079
  • EUR 105.00
  • EUR 244.00
  • 2 rxns
  • 50 rxns

FFPE Tissue Genomic DNA Kit
abx098866-50rxns 50 rxns
EUR 272

Zenoquick Genomic DNA Purification Kit
Z2003-200 200 preps
EUR 631

Zenoprep Genomic DNA Purification Kit
Z2004-400 400 preps
EUR 550

AccuRT Genomic DNA Removal Kit
G488 200 Reactions
EUR 82

Bacterial Genomic DNA Isolation Kit
K309-100
EUR 376

Cell & tissue genomic DNA extraction and purification kit
K1442-100
EUR 566

100 BP DNA LADDER, 500UL PER KIT
M-DNA-100BP 1/pk
EUR 73
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT
M-DNA-1KB 1/pk
EUR 70
Description: Bioscience Mol Bio; DNA Ladder

Human Brain Genomic DNA
X11001 10 µg Ask for price
Description: kits suitable for this type of research

Genomic DNA - Human Male
D1234999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Human Female
D1234999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Mouse Male
D1334999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Mouse Female
D1334999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Male
D1434999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Female
D1434999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Male
D1734999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Female
D1734999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Male
D1834999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Female
D1834999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Male
D1934999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Female
D1934999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Male
D1B34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Female
D1B34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Male
D1C34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Female
D1C34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Male
D1H34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Female
D1H34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Male
D1O34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Female
D1O34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Rapid Fungal Genomic DNA Isolation Kit
FT71415 50Preps
EUR 76.64

Easy3-Plant Genomic DNA Isolation Kit
FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit
FYG503-500P 500 Preps Ask for price

Rapid Bacteria Genomic DNA Isolation Kit
BS8225 50Preps
EUR 76.64

Rapid Yeast Genomic DNA Extraction Kit
BS8227 50Preps
EUR 76.64

Rapid Blood Genomic DNA Extraction Kit
BT4782 50Preps
EUR 76.64

Rapid Animal Genomic DNA Extraction Kit
AT4780 10Preps
EUR 69.03

Rapid Animal Genomic DNA Extraction Kit
AT4781 50Preps
EUR 126.13

Rapid Animal Genomic DNA Extraction Kit
AT4782 250preps
EUR 354.5

Genomic DNA Kit (No RNase A)
20-abx098862
  • EUR 398.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns

Plant Tissue Genomic DNA Isolation Kit
K316-100
EUR 376

Genomic DNA Extraction and Purification
EP10012 100 Tests
EUR 505

Genomic DNA Extraction KitAnimal Tissue
FYG102-100P 100 Preps Ask for price

 

 

Zenoquick Genomic DNA Purification Kit

 

Plasmid DNA purification from E. coli is a core approach for molecular cloning. Small scale purification (miniprep) from lower than 5 ml of bacterial tradition is a fast means for clone verification or DNA isolation, adopted by additional enzymatic reactions (polymerase chain response and restriction enzyme digestion).

 

Right here, we video-recorded the final procedures of miniprep via the QIAGEN’s QIAprep eight Miniprep Equipment, aiming to introducing this extremely environment friendly approach to the final learners for molecular biology strategies.

 

The entire process relies on alkaline lysis of E. coli cells adopted by adsorption of DNA onto silica within the presence of excessive salt.

 

Cell & tissue genomic DNA extraction and purification kit

Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

It consists of three steps: 1) preparation and clearing of a bacterial lysate, 2) adsorption of DNA onto the QIAprep membrane, 3) washing and elution of plasmid DNA.

 

Genomic DNA from Plant Regular Tissue: Arabidopsis
Description: Can be utilized for numerous research within the realm of gene expression, each regular and pathological. It is a superb management and appropriate for academic functions.  Can be utilized for numerous research within the realm of gene expression, each regular and pathological. It is a superb management and appropriate for academic functions.

 

 

All steps are carried out with out the use of phenol, chloroform, CsCl, ethidium bromide, and with out alcohol precipitation. It normally takes lower than 2 hours to complete your complete process.

 

Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification equipment, for era of huge quantities of plasmid DNA.

The GenomiPhi DNA Amplification Equipment employs rolling circle amplification (RCA) utilizing phi29 polymerase, dNTPs, and random hexamers. We demonstrated that repeated RCA (a minimum of 3 times) is helpful for high-fidelity amplification of huge quantities of plasmid DNA.

 

Toxin Child uncouples DNA replication and cell division to implement retention of plasmid R1 in Escherichia coli cells.

Worldwide dissemination of antibiotic resistance in micro organism is facilitated by plasmids that encode postsegregational killing (PSK) techniques.

 

These produce a secure toxin (T) and a labile antitoxin (A) conditioning cell survival to plasmid upkeep, as a result of solely this ensures neutralization of toxicity.

 

Scarcity of antibiotic options and the hyperlink of TA pairs to PSK have stimulated the opinion that untimely toxin activation could possibly be used to kill these recalcitrant organisms within the clinic.

 

Nevertheless, validation of TA pairs as therapeutic targets requires unambiguous understanding of their mode of motion, penalties for cell viability, and performance in plasmids.

Rapid Blood Genomic DNA Extraction Kit

Conflicting with widespread notions regarding these points, we had proposed that the TA pair kis-kid (killing suppressor-killing determinant) would possibly operate as a plasmid rescue system and never as a PSK system, however this remained to be validated.

 

Right here, we aimed to make clear unsettled mechanistic features of Child activation, and of the results of this for kis-kid-bearing plasmids and their host cells.

Easy3-Plant Genomic DNA Isolation Kit

We verify that activation of Child happens in cells which might be about to lose the toxin-encoding plasmid, and we present that this provokes extremely selective restriction of protein outputs that inhibits cell division briefly, avoiding plasmid loss, and stimulates DNA replication, selling plasmid rescue.

 

Kis and Child are conserved in plasmids encoding a number of antibiotic resistance genes, together with prolonged spectrum β-lactamases, for which therapeutic choices are scarce, and our findings advise in opposition to the activation of this TA pair to combat pathogens carrying these extrachromosomal DNAs.

 

 

 

 

Genomic DNA Extraction KitAnimal Tissue
FYG102-50P 50 Preps Ask for price

Genomic DNA Extraction Mini KitPlant Tissue
FYG103-100P 100 Preps Ask for price

Genomic DNA Kit
20-abx098076
  • EUR 439.00
  • EUR 244.00
  • 200 rxns
  • 50 rxns

Cell & tissue genomic DNA extraction and purification kit
K1442-100
EUR 566

Genomic DNA from Mouse Normal Tissue: Brain
D1334035 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Heart
D1334122 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Kidney
D1334142 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Liver
D1334149 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Lung
D1334152 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Spleen
D1334246 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Stomach
D1334248 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Brain
D1434035 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Heart
D1434122 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Kidney
D1434142 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Liver
D1434149 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Lung
D1434152 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Stomach
D1434248 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Testis
D1434260 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Thymus
D1434264 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Arabidopsis
D1634310 10 ug
EUR 493
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Arabidopsis
D1634310-5 5 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Corn
D1634330 10 ug
EUR 222
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Orange
D1634340 10 ug
EUR 222
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Potato
D1634350 10 ug
EUR 222
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Rice
D1634360 10 ug
EUR 222
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Wheat
D1634390 10 ug
EUR 222
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Tumor Tissue: Breast
D2235086 2 ug
EUR 821
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Tumor Tissue: Colon
D2235090 2 ug
EUR 821
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Tumor Tissue: Lung
D2235152 2 ug
EUR 821
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Tumor Tissue: Pancreas
D2235188 2 ug
EUR 821
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Tumor Tissue: Stomach
D2235248 2 ug
EUR 821
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Tumor Tissue: Uterus
D2235274 2 ug
EUR 821
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Plant Genomic DNA Kit
20-abx098077
  • EUR 411.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns

Bacteria Genomic DNA Kit
20-abx098080
  • EUR 105.00
  • EUR 244.00
  • 2 rxns
  • 50 rxns

Micro Genomic DNA Kit
20-abx098242
  • EUR 105.00
  • EUR 328.00
  • 2 rxns
  • 50 rxns

Blood Genomic DNA Kit
abx098868-50rxns 50 rxns
EUR 272

Plant Genomic DNA Kit
abx294004-50preps 50 preps
EUR 398