Best Commercial Plasmid DNA Kit

Genetici-technologies

Comparability of six business kits to extract bacterial chromosome and plasmid DNA for MiSeq sequencing.

We in contrast business kits for extraction of genomic DNA from the Gram-negative bacterium Klebsiella pneumoniae for subsequent Miseq sequencing.

 

Purification of DNA was based mostly on matrix binding (silica or anion alternate resin) or differential precipitation (salting out), respectively.

 

The selection of extraction equipment had little impact on sequencing high quality and protection throughout drastically completely different replicons, aside from an obvious depletion of small plasmids (<5 kb) throughout precipitation-based extractions.

 

Genomic DNA Isolation Kit | Soil Genomic DNA Kit

Description: Genomic DNA Isolation Kit for use in the research laboratory

 

Sequencing protection supplied copy-number estimates for small plasmids that have been persistently larger than these from quantitative real-time PCR.

Genetici-technologies
Genetici-technologies

Excessive effectivity technique to acquire supercoiled DNA with a business plasmid purification equipment.

Supercoiled state corresponds to the energetic type for plasmid purposes. The relaxed round type of plasmids is commonly inactive or poorly energetic.

 

To acquire vital quantities of virtually absolutely supercoiled DNA, we modified the usual protocol of a commercially accessible Qiagen plasmid purification equipment. Our modifications led to isolation of virtually 100% of the plasmids within the supercoiled state.

Fungal Genomic DNA Kit | Plant Genomic DNA Kit

The modified protocol was used to purify completely different plasmids with constant outcomes. The purified plasmids keep supercoiled state for about two months.

 

The modified protocol could be very advantageous as a result of it permits straightforward DNA manufacturing with excessive diploma of supercoiled type at low price.

 

Mammalian Cell Genomic DNA Isolation Kit
K967-100 each
EUR 457.2

Genomic DNA Maxi Kit (Blood/Cultured Cell)
GDM002 each
EUR 21

Genomic DNA Maxi Kit (Blood/Cultured Cell)
GDM010 each
EUR 89.3

Genomic DNA Maxi Kit (Blood/Cultured Cell)
GDM025 each
EUR 199.5

Genomic DNA Mini Kit (Blood/Cultured Cell)
GB004 each
EUR 3

Genomic DNA Mini Kit (Blood/Cultured Cell)
GB100 each
EUR 70

Genomic DNA Mini Kit (Blood/Cultured Cell)
GB300 each
EUR 189

Mbead Blood/Cell Genomic DNA Kit (MBeads Based)
MBS402046-100Reactions 100Reactions
EUR 330

Mbead Blood/Cell Genomic DNA Kit (MBeads Based)
MBS402046-4Reactions 4Reactions
EUR 185

Blood/Cell/Tissue Genomic DNA Extraction Kit
EP007-200T 200T
EUR 151

Blood/Cell/Tissue Genomic DNA Extraction Kit
EP007-50T 50T
EUR 54

Blood/Cell/Tissue Genomic DNA Extraction Kit
MBS8808202-200Tests 200Tests
EUR 340

Blood/Cell/Tissue Genomic DNA Extraction Kit
MBS8808202-50Tests 50Tests
EUR 215

Genomic DNA Kit
abx098076-100l 100 µl
EUR 300

Genomic DNA Kit
abx098076-1ml 1 ml Ask for price

Genomic DNA Kit
abx098076-200l 200 µl
EUR 550

Genomic DNA Kit
20-abx098076
  • Ask for price
  • Ask for price
  • 50 rxns
  • 200 rxns

GENOMIC DNA KIT
IB47250 2 X 96 PREP KIT
EUR 759.68

GENOMIC DNA KIT
IB47251 4 X 96 PREP KIT
EUR 1464.04

GENOMIC DNA KIT
IB47252 10 X 96 PREP KIT
EUR 3254.61

Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)
PDC09-0100 100 rxns
EUR 99

Biospin Blood/Cell/Tissue Genomic DNA Extraction Kit
TRI-C47M1 100T
EUR 252

Biospin Blood/Cell/Tissue Genomic DNA Extraction Kit
TRI-C47S1 50T
EUR 131.04

Genomic DNA - Human Tumor Cell Line: A431
D1255801 100 ug
EUR 207.2

Genomic DNA - Human Tumor Cell Line: Hela
D1255811 100 ug
EUR 207.2

Genomic DNA - Human Tumor Cell Line: Raji
D1255840 100 ug
EUR 207.2

Cell & tissue genomic DNA extraction and purification kit
K1442-100 each
EUR 679.2

Genomic DNA - Human Tumor Cell Line: Jurkat
D1255815 100 ug
EUR 207.2

Insta NX® Cell Genomic DNA Purification
MBIN011-12PR 1 unit
EUR 71.73
Description: Insta NX® Cell Genomic DNA Purification

Soil Genomic DNA Kit
K1411-250 each
EUR 673.2

Soil Genomic DNA Kit
K1411-50 each
EUR 379.2

Swab Genomic DNA Kit
MBS278036-50Preps 50Preps
EUR 245

Micro Genomic DNA Kit
abx098242-100l 100 µl
EUR 812.5

Micro Genomic DNA Kit
abx098242-1ml 1 ml Ask for price

Micro Genomic DNA Kit
abx098242-200l 200 µl Ask for price

Micro Genomic DNA Kit
20-abx098242
  • Ask for price
  • Ask for price
  • 2 rxns
  • 50 rxns

Blood Genomic DNA Kit
abx098868-100l 100 µl
EUR 337.5

Blood Genomic DNA Kit
abx098868-1ml 1 ml Ask for price

Blood Genomic DNA Kit
abx098868-200l 200 µl Ask for price

Blood Genomic DNA Kit
abx098868-50rxns 50 rxns
EUR 326.4

Plant Genomic DNA Kit
abx098077-100l 100 µl
EUR 287.5

Plant Genomic DNA Kit
abx098077-1ml 1 ml Ask for price

Plant Genomic DNA Kit
abx098077-200l 200 µl
EUR 512.5

Plant Genomic DNA Kit
20-abx098077
  • Ask for price
  • Ask for price
  • 50 rxns
  • 200 rxns

Plant Genomic DNA Kit
abx294004-100g 100 µg Ask for price

Plant Genomic DNA Kit
abx294004-20g 20 µg
EUR 312.5

Plant Genomic DNA Kit
abx294004-50g 50 µg Ask for price

Plant Genomic DNA Kit
abx294004-50preps 50 preps
EUR 477.6

Yeast Genomic DNA Kit
K1414-250 each
EUR 673.2

Yeast Genomic DNA Kit
K1414-50 each
EUR 379.2

Animal Genomic DNA Kit
abx294005-100g 100 µg Ask for price

Animal Genomic DNA Kit
abx294005-100preps 100 preps
EUR 627.6

Animal Genomic DNA Kit
abx294005-20g 20 µg
EUR 312.5

Animal Genomic DNA Kit
abx294005-50g 50 µg
EUR 725

Animal Genomic DNA Kit
abx294005-50preps 50 preps
EUR 477.6

Insect Genomic DNA Kit
K1412-250 each
EUR 673.2

Insect Genomic DNA Kit
K1412-50 each
EUR 379.2

Fungal Genomic DNA Kit
K1415-250 each
EUR 673.2

EcoPURE Genomic DNA Kit
E1075 50 rxn
EUR 60.5
Description: EcoPURE Genomic DNA Kit is designed as a simple and convenient purification of high quality genomic DNA from various samples including whole blood, cultured cells, frozen or fresh tissues, rodent tails, yeast, gram positive or gram negative bacteria, insects, dried blood spots, and buccal swaps. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 25 minutes and purified DNA can be used directly in PCR, qPCR, sequencing and enzymatic reactions.

Mollusc Genomic DNA Kit
K1413-250 each
EUR 673.2

Mollusc Genomic DNA Kit
K1413-50 each
EUR 379.2

Genomic DNA - Human Tumor Cell Line: K 562
D1255820 100 ug
EUR 207.2

Bacteria Genomic DNA Kit
abx098080-100l 100 µl
EUR 300

Bacteria Genomic DNA Kit
abx098080-1ml 1 ml Ask for price

Bacteria Genomic DNA Kit
abx098080-200l 200 µl Ask for price

Bacteria Genomic DNA Kit
20-abx098080
  • Ask for price
  • Ask for price
  • 2 rxns
  • 50 rxns

Genomic DNA - Human Tumor Cell Line: MCF 7
D1255830 100 ug
EUR 207.2

 

 

Zenoquick Genomic DNA Purification Kit

 

Plasmid DNA purification from E. coli is a core approach for molecular cloning. Small scale purification (miniprep) from lower than 5 ml of bacterial tradition is a fast means for clone verification or DNA isolation, adopted by additional enzymatic reactions (polymerase chain response and restriction enzyme digestion).

 

Right here, we video-recorded the final procedures of miniprep via the QIAGEN’s QIAprep eight Miniprep Equipment, aiming to introducing this extremely environment friendly approach to the final learners for molecular biology strategies.

 

The entire process relies on alkaline lysis of E. coli cells adopted by adsorption of DNA onto silica within the presence of excessive salt.

 

Cell & tissue genomic DNA extraction and purification kit

Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

It consists of three steps: 1) preparation and clearing of a bacterial lysate, 2) adsorption of DNA onto the QIAprep membrane, 3) washing and elution of plasmid DNA.

 

Genomic DNA from Plant Regular Tissue: Arabidopsis
Description: Can be utilized for numerous research within the realm of gene expression, each regular and pathological. It is a superb management and appropriate for academic functions.  Can be utilized for numerous research within the realm of gene expression, each regular and pathological. It is a superb management and appropriate for academic functions.

 

 

All steps are carried out with out the use of phenol, chloroform, CsCl, ethidium bromide, and with out alcohol precipitation. It normally takes lower than 2 hours to complete your complete process.

 

Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification equipment, for era of huge quantities of plasmid DNA.

The GenomiPhi DNA Amplification Equipment employs rolling circle amplification (RCA) utilizing phi29 polymerase, dNTPs, and random hexamers. We demonstrated that repeated RCA (a minimum of 3 times) is helpful for high-fidelity amplification of huge quantities of plasmid DNA.

 

Toxin Child uncouples DNA replication and cell division to implement retention of plasmid R1 in Escherichia coli cells.

Worldwide dissemination of antibiotic resistance in micro organism is facilitated by plasmids that encode postsegregational killing (PSK) techniques.

 

These produce a secure toxin (T) and a labile antitoxin (A) conditioning cell survival to plasmid upkeep, as a result of solely this ensures neutralization of toxicity.

 

Scarcity of antibiotic options and the hyperlink of TA pairs to PSK have stimulated the opinion that untimely toxin activation could possibly be used to kill these recalcitrant organisms within the clinic.

 

Nevertheless, validation of TA pairs as therapeutic targets requires unambiguous understanding of their mode of motion, penalties for cell viability, and performance in plasmids.

Rapid Blood Genomic DNA Extraction Kit

Conflicting with widespread notions regarding these points, we had proposed that the TA pair kis-kid (killing suppressor-killing determinant) would possibly operate as a plasmid rescue system and never as a PSK system, however this remained to be validated.

 

Right here, we aimed to make clear unsettled mechanistic features of Child activation, and of the results of this for kis-kid-bearing plasmids and their host cells.

Easy3-Plant Genomic DNA Isolation Kit

We verify that activation of Child happens in cells which might be about to lose the toxin-encoding plasmid, and we present that this provokes extremely selective restriction of protein outputs that inhibits cell division briefly, avoiding plasmid loss, and stimulates DNA replication, selling plasmid rescue.

 

Kis and Child are conserved in plasmids encoding a number of antibiotic resistance genes, together with prolonged spectrum β-lactamases, for which therapeutic choices are scarce, and our findings advise in opposition to the activation of this TA pair to combat pathogens carrying these extrachromosomal DNAs.

 

 

 

 

Mbead Tissue Genomic DNA Kit
PDM02-0100 100 rxns
EUR 154

EcoSpin Tissue Genomic DNA Kit
E1070 50 rxn
EUR 53.9
Description: EcoSpin Tissue Genomic DNA Kit is designed as a simple and convenient purification of high quality genomic DNA from fresh or frozen tissue samples and rodent tails. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 30 minutes and purified DNA can be used directly in PCR, qPCR, sequencing and enzymatic reactions.

Genomic DNA Mini Kit (Tissue)
GT004 each
EUR 3

Genomic DNA Mini Kit (Tissue)
GT050 each
EUR 63.5

Genomic DNA Mini Kit (Tissue)
GT100 each
EUR 105

Genomic DNA Mini Kit (Tissue)
GT300 each
EUR 283.5

Genomic DNA Isolation Kit (Tissue)
PDC11-0100 100 rxns
EUR 132

Dual Genomic DNA Isolation Kit(Tissue)
PDC06-0100 100 rxns
EUR 154

Plant Tissue Genomic DNA Isolation Kit
K316-100 each
EUR 451.2

Cells/Tissue genomic DNA Extraction kit
2509A 50 Tests
EUR 80

Cells/Tissue genomic DNA Extraction kit
2509B 100 Tests
EUR 100

Cells/Tissue genomic DNA Extraction kit
2509C 2*100 Tests
EUR 150

Cells/Tissue genomic DNA Extraction kit
2510A 20 Tests
EUR 250

Cells/Tissue genomic DNA Extraction kit
2510B 50 Tests
EUR 450

Biospin Tissue Genomic DNA Extraction Kit
TRI-C04M1 100T
EUR 272.16

Biospin Tissue Genomic DNA Extraction Kit
TRI-C04S1 50T
EUR 141.12

BioFast Tissue Genomic DNA Extraction Kit
TRI-C19S1 50T
EUR 211.68

Mbead Tissue Genomic DNA Kit (MBeads Based)
MBS402042-100Reactions 100Reactions
EUR 330

Mbead Tissue Genomic DNA Kit (MBeads Based)
MBS402042-4Reactions 4Reactions
EUR 185

Genomic DNA Extraction KitAnimal Tissue
FYG102-100P 100 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue
FYG102-300P 300 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue
FYG102-50P 50 Preps Ask for price

Magnetic Beads-Based Tissue Genomic DNA Kit
abx460016-1096tests 10 × 96 tests Ask for price

Magnetic Beads-Based Tissue Genomic DNA Kit
abx460016-596tests 5 × 96 tests Ask for price

Magnetic Beads-Based Tissue Genomic DNA Kit
abx460016-96tests 96 tests
EUR 437.5

BioFastspin Tissue Genomic DNA Extraction Kit
TRI-C20S1 50T
EUR 231.84

Genomic DNA Isolation Kit (Tissue) (Column Based)
MBS402038-100Reactions 100Reactions
EUR 305

Genomic DNA Isolation Kit (Tissue) (Column Based)
MBS402038-4Reactions 4Reactions
EUR 185

Blood/Cell/Tissue Genomic DNA Extraction Kit
EP007-200T 200T
EUR 151

Blood/Cell/Tissue Genomic DNA Extraction Kit
EP007-50T 50T
EUR 54

Blood/Cell/Tissue Genomic DNA Extraction Kit
MBS8808202-200Tests 200Tests
EUR 340

Blood/Cell/Tissue Genomic DNA Extraction Kit
MBS8808202-50Tests 50Tests
EUR 215

Genomic DNA - Rat Normal Tissue: Lung
D1434152 100 ug
EUR 241.5

Genomic DNA - Rat Normal Tissue: Brain
D1434035 100 ug
EUR 241.5

Genomic DNA - Rat Normal Tissue: Heart
D1434122 100 ug
EUR 241.5

Genomic DNA - Rat Normal Tissue: Liver
D1434149 100 ug
EUR 241.5

Biospin FFPE Tissue Genomic DNA Extraction Kit
TRI-C24M1 100T
EUR 650.16