Best Commercial Plasmid DNA Kit

Genetici-technologies

Comparability of six business kits to extract bacterial chromosome and plasmid DNA for MiSeq sequencing.

We in contrast business kits for extraction of genomic DNA from the Gram-negative bacterium Klebsiella pneumoniae for subsequent Miseq sequencing.

 

Purification of DNA was based mostly on matrix binding (silica or anion alternate resin) or differential precipitation (salting out), respectively.

 

The selection of extraction equipment had little impact on sequencing high quality and protection throughout drastically completely different replicons, aside from an obvious depletion of small plasmids (<5 kb) throughout precipitation-based extractions.

 

Genomic DNA Isolation Kit | Soil Genomic DNA Kit

Description: Genomic DNA Isolation Kit for use in the research laboratory

 

Sequencing protection supplied copy-number estimates for small plasmids that have been persistently larger than these from quantitative real-time PCR.

Genetici-technologies
Genetici-technologies

Excessive effectivity technique to acquire supercoiled DNA with a business plasmid purification equipment.

Supercoiled state corresponds to the energetic type for plasmid purposes. The relaxed round type of plasmids is commonly inactive or poorly energetic.

 

To acquire vital quantities of virtually absolutely supercoiled DNA, we modified the usual protocol of a commercially accessible Qiagen plasmid purification equipment. Our modifications led to isolation of virtually 100% of the plasmids within the supercoiled state.

Fungal Genomic DNA Kit | Plant Genomic DNA Kit

The modified protocol was used to purify completely different plasmids with constant outcomes. The purified plasmids keep supercoiled state for about two months.

 

The modified protocol could be very advantageous as a result of it permits straightforward DNA manufacturing with excessive diploma of supercoiled type at low price.

 

Plant Genomic DNA Kit
20-abx098077
  • EUR 493.20
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Micro Genomic DNA Kit
20-abx098242
  • EUR 126.00
  • EUR 393.60
  • 2 rxns
  • 50 rxns

Blood Genomic DNA Kit
abx098868-50rxns 50 rxns
EUR 326.4

Plant Genomic DNA Kit
abx294004-50preps 50 preps
EUR 477.6

Yeast Genomic DNA Kit
K1414-250 each
EUR 673.2

Yeast Genomic DNA Kit
K1414-50 each
EUR 379.2

Animal Genomic DNA Kit
abx294005-100preps 100 preps
EUR 627.6

Animal Genomic DNA Kit
abx294005-50preps 50 preps
EUR 477.6

Insect Genomic DNA Kit
K1412-250 each
EUR 673.2

Insect Genomic DNA Kit
K1412-50 each
EUR 379.2

Fungal Genomic DNA Kit
K1415-250 each
EUR 673.2

Fungal Genomic DNA Kit
K1415-50 each
EUR 379.2

Mollusc Genomic DNA Kit
K1413-250 each
EUR 673.2

Mollusc Genomic DNA Kit
K1413-50 each
EUR 379.2

Bacteria Genomic DNA Kit
20-abx098080
  • EUR 126.00
  • EUR 292.80
  • 2 rxns
  • 50 rxns

Genomic DNA Isolation Kit
55R-1362 50 assays
EUR 462
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation Kit
K281-50 each
EUR 326.4

Genomic DNA Isolation Kit
K2118-50 50 assays
EUR 385.2

ISOLATE II Genomic DNA Kit
BIO-52065 10 preps Ask for price

ISOLATE II Genomic DNA Kit
BIO-52066 50 preps Ask for price

ISOLATE II Genomic DNA Kit
BIO-52067 250 preps Ask for price

FFPE Tissue Genomic DNA Kit
abx098866-50rxns 50 rxns
EUR 326.4

Genomic DNA - Human Tumor Cell Line: A431
D1255801 100 ug
EUR 245

Genomic DNA - Human Tumor Cell Line: Hela
D1255811 100 ug
EUR 245

Genomic DNA - Human Tumor Cell Line: Raji
D1255840 100 ug
EUR 245

Marine Animal Genomic DNA Kit
20-abx098079
  • EUR 126.00
  • EUR 292.80
  • 2 rxns
  • 50 rxns

Human Genomic DNA
BIO-35025 500µl @ 200ng/µl Ask for price

Human Genomic DNA 
X11000
  • Ask for price
  • EUR 235.40
  • 0.2 ml
  • 0.2 ml

Sera-Xtracta Genomic DNA Kit
29429140 EACH
EUR 574.8

Genomic DNA - Human Tumor Cell Line: Jurkat
D1255815 100 ug
EUR 245

Genomic DNA Kit (No RNase A)
20-abx098862
  • EUR 477.60
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Cell & tissue genomic DNA extraction and purification kit
K1442-100 each
EUR 679.2

Genomic DNA - Human Tumor Cell Line: K 562
D1255820 100 ug
EUR 245

Genomic DNA - Human Tumor Cell Line: MCF 7
D1255830 100 ug
EUR 245

AccuRT Genomic DNA Removal Kit
G488 200 Reactions
EUR 98.4

Blood Genomic DNA Kit (5-250 ul)
20-abx098078
  • EUR 493.20
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-For200mlBlood For 200 ml Blood
EUR 427.2

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-For50mlBlood For 50 ml Blood
EUR 260.4

Bacterial Genomic DNA Isolation Kit
K309-100 each
EUR 451.2

Human Brain Genomic DNA  
X11001
  • Ask for price
  • EUR 77.00
  • 10 µg
  • 10 ul

Zenoprep Genomic DNA Purification Kit
Z2004-400 400 preps
EUR 660

Plant Genomic DNA Kit(No RNase A)
20-abx098863
  • EUR 460.80
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (No RNase A)
20-abx098864
  • EUR 460.80
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Zenoquick Genomic DNA Purification Kit
Z2003-200 200 preps
EUR 757.2

Bacteria Genomic DNA Kit (No RNase A)
abx098240-50rxns 50 rxns
EUR 276

T-Pro Genomic DNA Mini Kit (100)
RB94-NGS100 100preps/kit
EUR 255.6

T-Pro Genomic DNA Midi Kit (20)
RB94-NGM020 20preps/kit
EUR 266.4

Food and Fodder Security Genomic DNA Kit
abx098241-50rxns 50 rxns
EUR 376.8

Rapid Blood Genomic DNA Extraction Kit
BT4782 50Preps
EUR 91.97

Rapid Yeast Genomic DNA Extraction Kit
BS8227 50Preps
EUR 91.97

Rapid Fungal Genomic DNA Isolation Kit
FT71415 50Preps
EUR 91.97

Plant Tissue Genomic DNA Isolation Kit
K316-100 each
EUR 451.2

Blood Genomic DNA Kit (with Magnetic Stand)
abx098867-50rxns 50 rxns
EUR 360

Rapid Animal Genomic DNA Extraction Kit
AT4780 10Preps
EUR 82.84

Rapid Animal Genomic DNA Extraction Kit
AT4781 50Preps
EUR 151.36

Rapid Animal Genomic DNA Extraction Kit
AT4782 250preps
EUR 425.4

Easy3-Plant Genomic DNA Isolation Kit
FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit
FYG503-500P 500 Preps Ask for price

Rapid Bacteria Genomic DNA Isolation Kit
BS8225 50Preps
EUR 91.97

Nucleon BACC3 Genomic DNA Extraction Kit
RPN8512 EACH
EUR 484.8

Control Genomic DNA - Rat Male
D1434999-G01 100 ug
EUR 170

Control Genomic DNA - Dog Male
D1734999-G01 100 ug
EUR 170

Marine Animal Genomic DNA Kit (No RNase A)
abx298012-50rxns 50 rxns
EUR 276

One-4-All Genomic DNA Miniprep Kit
BS88503 50Preps
EUR 140.39

One-4-All Genomic DNA Miniprep Kit
BS88504 100preps
EUR 204.7

One-4-All Genomic DNA Miniprep Kit
BS88505 250preps
EUR 393.55

Mouse Tail Genomic DNA Extraction Kit(250)
M9100-250 250 prep
EUR 643.2

 

 

Zenoquick Genomic DNA Purification Kit

 

Plasmid DNA purification from E. coli is a core approach for molecular cloning. Small scale purification (miniprep) from lower than 5 ml of bacterial tradition is a fast means for clone verification or DNA isolation, adopted by additional enzymatic reactions (polymerase chain response and restriction enzyme digestion).

 

Right here, we video-recorded the final procedures of miniprep via the QIAGEN’s QIAprep eight Miniprep Equipment, aiming to introducing this extremely environment friendly approach to the final learners for molecular biology strategies.

 

The entire process relies on alkaline lysis of E. coli cells adopted by adsorption of DNA onto silica within the presence of excessive salt.

 

Cell & tissue genomic DNA extraction and purification kit

Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

It consists of three steps: 1) preparation and clearing of a bacterial lysate, 2) adsorption of DNA onto the QIAprep membrane, 3) washing and elution of plasmid DNA.

 

Genomic DNA from Plant Regular Tissue: Arabidopsis
Description: Can be utilized for numerous research within the realm of gene expression, each regular and pathological. It is a superb management and appropriate for academic functions.  Can be utilized for numerous research within the realm of gene expression, each regular and pathological. It is a superb management and appropriate for academic functions.

 

 

All steps are carried out with out the use of phenol, chloroform, CsCl, ethidium bromide, and with out alcohol precipitation. It normally takes lower than 2 hours to complete your complete process.

 

Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification equipment, for era of huge quantities of plasmid DNA.

The GenomiPhi DNA Amplification Equipment employs rolling circle amplification (RCA) utilizing phi29 polymerase, dNTPs, and random hexamers. We demonstrated that repeated RCA (a minimum of 3 times) is helpful for high-fidelity amplification of huge quantities of plasmid DNA.

 

Toxin Child uncouples DNA replication and cell division to implement retention of plasmid R1 in Escherichia coli cells.

Worldwide dissemination of antibiotic resistance in micro organism is facilitated by plasmids that encode postsegregational killing (PSK) techniques.

 

These produce a secure toxin (T) and a labile antitoxin (A) conditioning cell survival to plasmid upkeep, as a result of solely this ensures neutralization of toxicity.

 

Scarcity of antibiotic options and the hyperlink of TA pairs to PSK have stimulated the opinion that untimely toxin activation could possibly be used to kill these recalcitrant organisms within the clinic.

 

Nevertheless, validation of TA pairs as therapeutic targets requires unambiguous understanding of their mode of motion, penalties for cell viability, and performance in plasmids.

Rapid Blood Genomic DNA Extraction Kit

Conflicting with widespread notions regarding these points, we had proposed that the TA pair kis-kid (killing suppressor-killing determinant) would possibly operate as a plasmid rescue system and never as a PSK system, however this remained to be validated.

 

Right here, we aimed to make clear unsettled mechanistic features of Child activation, and of the results of this for kis-kid-bearing plasmids and their host cells.

Easy3-Plant Genomic DNA Isolation Kit

We verify that activation of Child happens in cells which might be about to lose the toxin-encoding plasmid, and we present that this provokes extremely selective restriction of protein outputs that inhibits cell division briefly, avoiding plasmid loss, and stimulates DNA replication, selling plasmid rescue.

 

Kis and Child are conserved in plasmids encoding a number of antibiotic resistance genes, together with prolonged spectrum β-lactamases, for which therapeutic choices are scarce, and our findings advise in opposition to the activation of this TA pair to combat pathogens carrying these extrachromosomal DNAs.

 

 

 

 

Genomic DNA Extraction KitAnimal Tissue
FYG102-50P 50 Preps Ask for price

Genomic DNA - Rat Normal Tissue: Lung
D1434152 100 ug
EUR 286

Genomic DNA - Rat Normal Tissue: Brain
D1434035 100 ug
EUR 286

Genomic DNA - Rat Normal Tissue: Heart
D1434122 100 ug
EUR 286

Genomic DNA - Rat Normal Tissue: Liver
D1434149 100 ug
EUR 286

Genomic DNA - Mouse Normal Tissue: Lung
D1334152 100 ug
EUR 286

Genomic DNA - Rat Normal Tissue: Kidney
D1434142 100 ug
EUR 286

Genomic DNA - Rat Normal Tissue: Testis
D1434260 100 ug
EUR 286

Genomic DNA - Rat Normal Tissue: Thymus
D1434264 100 ug
EUR 286

Genomic DNA - Plant Normal Tissue: Corn
D1634330 10 ug
EUR 212

Genomic DNA - Plant Normal Tissue: Rice
D1634360 10 ug
EUR 212

Genomic DNA - Mouse Normal Tissue: Brain
D1334035 100 ug
EUR 286

Genomic DNA - Mouse Normal Tissue: Heart
D1334122 100 ug
EUR 286

Genomic DNA - Mouse Normal Tissue: Liver
D1334149 100 ug
EUR 286

Genomic DNA - Rat Normal Tissue: Stomach
D1434248 100 ug
EUR 286

Genomic DNA - Plant Normal Tissue: Wheat
D1634390 10 ug
EUR 212

Genomic DNA - Mouse Normal Tissue: Kidney
D1334142 100 ug
EUR 286

Genomic DNA - Mouse Normal Tissue: Spleen
D1334246 100 ug
EUR 286

Genomic DNA - Plant Normal Tissue: Orange
D1634340 10 ug
EUR 212

Genomic DNA - Plant Normal Tissue: Potato
D1634350 10 ug
EUR 212

Genomic DNA Extraction Mini KitPlant Tissue
FYG103-100P 100 Preps Ask for price

Genomic DNA - Mouse Normal Tissue: Stomach
D1334248 100 ug
EUR 286

Genomic DNA - Plant Normal Tissue: Arabidopsis
D1634310 10 ug
EUR 547

Genomic DNA - Plant Normal Tissue: Arabidopsis
D1634310-5 5 ug
EUR 286

Genomic DNA - Plant Normal Tissue: Soy bean
D1634370 10 ug
EUR 212

FFPE Genomic DNA - Human Tumor Tissue: Lung
D2235152 2 ug
EUR 960

FFPE Genomic DNA - Human Tumor Tissue: Colon
D2235090 2 ug
EUR 960

FFPE Genomic DNA - Human Tumor Tissue: Breast
D2235086 2 ug
EUR 960

FFPE Genomic DNA - Human Tumor Tissue: Uterus
D2235274 2 ug
EUR 960

FFPE Genomic DNA - Human Tumor Tissue: Stomach
D2235248 2 ug
EUR 960

FFPE Genomic DNA - Human Tumor Tissue: Pancreas
D2235188 2 ug
EUR 960

Genomic DNA - Rat Normal Tissue: Skeletal Muscle
D1434171 100 ug
EUR 286

Genomic DNA - Rat Normal Tissue: Small Intestine
D1434226 100 ug
EUR 286

Cell & tissue genomic DNA extraction and purification kit
K1442-100 each
EUR 679.2

Genomic DNA - Mouse Normal Tissue: Skeletal Muscle
D1334171 100 ug
EUR 286

Genomic DNA - Mouse Normal Tissue: Small Intestine
D1334226 100 ug
EUR 286

FFPE Genomic DNA - Human Adult Normal Tissue: Lung
D2234152 2 ug
EUR 684