Accurate method of detecting DNA, Klentaq

Genetici-technologies

A quick and correct technique of detecting Aleutian mink illness virus in blood and tissues of chronically contaminated mink.

 

 

 

The target of this research was to evaluate the sensitivity of the Omni Klentaq–LA DNA polymerase for detecting Aleutian mink illness virus (AMDV) in mink blood and tissues by PCR with out DNA extraction.

  • The presence of AMDV DNA was instantly examined by Klentaq within the plasma, serum, complete blood, and spleen homogenates of 188 mink Four and 16 months after inoculation with the virus.

 

  • Samples from bone marrow, small gut, liver, lungs, kidneys, and lymph nodes of 20 of the identical mink have been additionally examined by Klentaq. DNA was extracted from paired samples of plasma and the aforesaid tissues by a industrial nucleic acid extraction package (Dynabeads Silane) and examined by PCR.

 

  • In contrast with the extracted DNA, Klentaq detected a considerably higher variety of samples in the entire blood, serum, plasma, spleen, and small gut. It was concluded that Klentaq is a most well-liked system for instantly detecting AMDV DNA in mink blood and tissues.

 

  • The decrease success fee of extracted DNA in contrast with Klentaq could possibly be the results of DNA losses throughout the extraction course of. This is a crucial think about chronically contaminated mink, which have a low AMDV copy quantity within the bloodstream.
  • Direct AMDV detection additionally reduces the price of PCR amplification and lowers the chance of pattern contamination.

 

 

Genetici-technologies
Genetici-technologies

 

mPEG-LA
abx085219-20kDa1g 20 kDa; 1 g
EUR 565
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mPEG-LA
abx085219-2kDa1g 2 kDa; 1 g
EUR 537
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mPEG-LA
abx085219-5kDa1g 5 kDa; 1 g
EUR 537
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DCP-LA
HY-108599 10mg
EUR 291
SSB La protein
30-AS74 200 ug
EUR 570
Description: Purified native Bovine SSB La protein
Lupus La Antibody
20-abx133336
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
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LA-PEG-NH2
abx085239-2kDa1g 2 kDa; 1 g
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LA-PEG-NH2
abx085239-34kDa1g 3.4 kDa; 1 g
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LA-PEG-NH2
abx085239-34kDa5g 3.4 kDa; 5 g
EUR 1817
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LA-PEG-NH2
abx085239-5kDa1g 5 kDa; 1 g
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LA-PEG-Biotin
abx085329-34kDa1g 3.4 kDa; 1 g
EUR 773
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LA-PEG-Biotin
abx085329-34kDa5g 3.4 kDa; 5 g
EUR 1970
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LA-PEG-Biotin
abx085329-5kDa1g 5 kDa; 1 g
EUR 773
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LA-PEG-Biotin
abx085329-5kDa5g 5 kDa; 5 g
EUR 1970
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LA-PEG-COOH
abx085330-34kDa1g 3.4 kDa; 1 g
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LA-PEG-COOH
abx085330-34kDa5g 3.4 kDa; 5 g
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LA-PEG-COOH
abx085330-5kDa1g 5 kDa; 1 g
EUR 718
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LA-PEG-COOH
abx085330-5kDa5g 5 kDa; 5 g
EUR 1817
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LA-PEG-NHS
abx085331-34kDa1g 3.4 kDa; 1 g
EUR 718
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LA-PEG-NHS
abx085331-34kDa5g 3.4 kDa; 5 g
EUR 1817
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LA-PEG-NHS
abx085331-5kDa1g 5 kDa; 1 g
EUR 718
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LA-PEG-NHS
abx085331-5kDa5g 5 kDa; 5 g
EUR 1817
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LA-PEG-NH2,2K
33-HE039005-2K
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  • EUR 1345.00
  • 1g
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
LA-PEG-NH2,3.4K
33-HE039005-3.4K
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  • EUR 1345.00
  • 1g
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
LA-PEG-NH2,4K
33-HE039005-4K
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  • 1g
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
LA-PEG-NH2,5K
33-HE039005-5K
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
mPEG-LA,10K
33-MF001039-10K
  • EUR 359.00
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
mPEG-LA,1K
33-MF001039-1K
  • EUR 322.00
  • EUR 864.00
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
mPEG-LA,20K
33-MF001039-20K
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
mPEG-LA,2K
33-MF001039-2K
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mPEG-LA,5K
33-MF001039-5K
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  • EUR 790.00
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
mPEG-LA, 750
33-MF001039-750
  • EUR 346.00
  • EUR 938.00
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
LA/SS-B LA / SS-B Human Recombinant Protein
PROTP05455 Regular: 20ug
EUR 317
Description: LA/SS-B Human Recombinant produced in SF9 is a single, glycosylated, polypeptide chain having a calculated molecular mass of 52 kDa, is fused to a hexahistidine purification tag.;The LA/SS-B is purified by proprietary chromatographic techniques.
La Ribonucleoprotein (SSB) Antibody
31239-05111 150 ug
EUR 261
Lupus La (pS366) Antibody
20-abx134921
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  • EUR 230.00
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Lupus La protein Antibody
20-abx109971
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  • EUR 1845.00
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  • EUR 300.00
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LA/SS-B Protein
abx160021-1mg 1 mg
EUR 1678
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Lupus La Blocking Peptide
20-abx162045
  • EUR 272.00
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SS-B (La) Protein
abx069945-02mg 0.2 mg
EUR 1156
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SS-B (La) Protein
abx069946-1000Units 1000 Units
EUR 1483
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SS-B (La) Protein
abx069947-100ug 100 ug
EUR 599
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LA / SS-B Protein
20-abx261802
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  • EUR 328.00
  • EUR 230.00
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Human LA ELISA Kit
EHL0011 96Tests
EUR 521
Bovine LA ELISA Kit
EBL0011 96Tests
EUR 521
Chicken LA ELISA Kit
ECKL0011 96Tests
EUR 521
Anserini LA ELISA Kit
EAL0011 96Tests
EUR 521
Canine LA ELISA Kit
ECL0011 96Tests
EUR 521
Goat LA ELISA Kit
EGTL0011 96Tests
EUR 521
LA-PEG-COOH,2K
33-HE039017-2K
  • EUR 482.00
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
LA-PEG-COOH,3.4K
33-HE039017-3.4K
  • EUR 482.00
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
LA-PEG-COOH,5K
33-HE039017-5K
  • EUR 482.00
  • EUR 1345.00
  • 1g
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
LA-PEG-NHS,3.4K
33-HE039023-3.4K
  • EUR 482.00
  • EUR 1345.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
LA-PEG-NHS,5K
33-HE039023-5K
  • EUR 482.00
  • EUR 1345.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
LA-PEG-Biotin,2K
33-HE039041-2K
  • EUR 519.00
  • EUR 1456.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
LA-PEG-Biotin,3.4K
33-HE039041-3.4K
  • EUR 519.00
  • EUR 1456.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
LA-PEG-Biotin,5K
33-HE039041-5K
  • EUR 519.00
  • EUR 1456.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
Porcine LA ELISA Kit
EPL0011 96Tests
EUR 521
Sheep LA ELISA Kit
ESL0011 96Tests
EUR 521
Rat LA ELISA Kit
ERL0011 96Tests
EUR 521
Rabbit LA ELISA Kit
ERTL0011 96Tests
EUR 521
Monkey LA ELISA Kit
EMKL0011 96Tests
EUR 521
Mouse LA ELISA Kit
EML0011 96Tests
EUR 521
Human ? Lactalbumin,?-La ELISA Kit
201-12-1850 96 tests
EUR 440
  • This alpha Lactalbumin ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.
Recombinant Human LA / SS-B
7-05473 5µg Ask for price
Recombinant Human LA / SS-B
7-05474 20µg Ask for price
Recombinant Human LA / SS-B
7-05475 1mg Ask for price
Lupus La protein Polyclonal Antibody
42333-100ul 100ul
EUR 333

 

Evaluating the efficacy of assorted thermo-stable polymerases in opposition to co-extracted PCR inhibitors in historic DNA samples.

 

DNA from historic and forensic specimens is usually co-extracted with unknown quantities of unknown PCR inhibitors, which might result in underestimated DNA concentrations, allelic drop-out, and/or false-negative outcomes.

 

LA-PEG-NHS,5K | LA-PEG-Biotin,2K

Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture.

In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

 

It isn’t shocking, on this case, that quite a few strategies have been developed to take away PCR inhibitors or subdue their results. One easy and price efficient strategy could possibly be the adoption of a polymerase that overcomes or is much less affected by PCR inhibitors.

 

On this research, 9 completely different polymerases have been evaluated for his or her efficacy in opposition to PCR inhibitors co-extracted with DNA from 63 historic salmon vertebrae. These samples have been excavated from two archeological websites positioned on the Dionisio Level locality on the northern finish of Galiano Island in coastal southwestern British Columbia, Canada and date to 700-1000 and 1300-1500 years earlier than current.

 

LA-PEG-NH2,2K | LA-PEG-Biotin,5K

Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture.

 

In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Description: A excessive purity chemical with numerous functions in medical analysis, drug-release, nanotechnology and new supplies analysis, cell tradition. Within the research of ligand, polypeptide synthesis help, a graft polymer compounds, new supplies, and polyethylene glycol-modified practical coatings and different elements of the energetic compound.

A excessive purity chemical with numerous functions in medical analysis, drug-release, nanotechnology and new supplies analysis, cell tradition. Within the research of ligand, polypeptide synthesis help, a graft polymer compounds, new supplies, and polyethylene glycol-modified practical coatings and different elements of the energetic compound.

 

 

Beforehand, DNA extracts from samples studied from this locality have been decided to be largely inhibited to PCR amplification.

 

Within the current research, Omni Klentaq LA (DNA Polymerase Expertise, Inc.) outperformed the opposite eight polymerases in two measures: (1) its success in genetic species identification of those vertebrae, and (2) its means to amplify an historic DNA constructive management when spiked with a quantity of probably inhibited extract from the vertebrae.

 

Lupus La Protein (SSB) Antibody | Bovine LA ELISA Kit

 

Escherichia coli polymerase 1 (Pol 1) and Thermus aquaticus Taq polymerase are homologous Sort I DNA polymerases, every comprised of a polymerase area, a proofreading area (inactive in Taq), and a 5′ nuclease area.

 

“Klenow” and “Klentaq” are the big fragments of Pol 1 and Taq and are practical polymerases missing the 5′ nuclease area. Within the accessible crystal constructions of full-length Taq, the 5′ nuclease area is positioned in two completely different orientations: in a single construction, it’s prolonged out into resolution, whereas within the different, it’s folded up in opposition to the polymerase area in a extra compact construction.

 

Analytical ultracentrifugation experiments report s20,w values of 5.05 for Taq, 4.1 for Klentaq, 5.three for E. coli Pol 1, and 4.6 for Klenow. Measured partial particular volumes are all fairly related, indicating no important variations in packing density between the mesophilic and thermophilic proteins.

 

Small angle x-ray scattering research report radii of gyration of 38.three A for Taq, 30.7 A for Klentaq, and 30.5 A for Klenow. The hydrodynamic and x-ray scattering properties of the polymerases have been additionally calculated instantly from the completely different crystal constructions utilizing the applications HYDROPRO (Garcia De La Torre, J., Huertas, M. L., and Carrasco, B. (2000) Biophys J. 78, 719-730) and CRYSOL (Svergun, D. I., Barberato, C., and Koch, M. H. J. (1995) J. Appl. Crystalogr. 28, 768-773), respectively.

 

The mixed experimental and computational characterizations point out that the full-length polymerases in resolution are in a conformation the place the 5′ nuclease area is prolonged into resolution. Additional, the radius of gyration, and therefore the worldwide conformation of Taq polymerase, just isn’t altered by the binding of both matched primer template DNA or ddATP.

 

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria

Halobacillus PCR kit
PCR-A635-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Halobacillus

Halobacillus PCR kit
PCR-A635-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Halobacillus

Enterovirus PCR kit
PCR-H433-48R 50T
EUR 524.4
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Description: An conventional PCR kit for detection of Enterovirus

Enterovirus PCR kit
PCR-H433-96R 100T
EUR 669.4
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Description: An conventional PCR kit for detection of Enterovirus

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PCR-H453-48R 50T
EUR 524.4
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Description: An conventional PCR kit for detection of Mengovirus

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PCR-H453-96R 100T
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Description: An conventional PCR kit for detection of Mengovirus

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PCR-H491-48R 50T
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Description: An conventional PCR kit for detection of Astrovirus

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PCR-H491-96R 100T
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PCR-H493-48R 50T
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Description: An conventional PCR kit for detection of Calicivirus

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Description: An conventional PCR kit for detection of Calicivirus

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PCR-H501-48D 50T
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Description: An conventional PCR kit for detection of Adenovirus

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PCR-H501-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Adenovirus

Polyomavirus PCR kit
PCR-H515-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Polyomavirus

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PCR-H515-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Polyomavirus

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PCR-H630-48R 50T
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Description: An conventional PCR kit for detection of Sapovirus

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PCR-H630-96R 100T
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Description: An conventional PCR kit for detection of Sapovirus

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PCR-H642-48R 50T
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Description: An conventional PCR kit for detection of Parechovirus

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PCR-H642-96R 100T
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Description: An conventional PCR kit for detection of Parechovirus

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PCR-H643-48R 50T
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Description: An conventional PCR kit for detection of Metapneumovirus

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Description: An conventional PCR kit for detection of Metapneumovirus

Entamoeba PCR kit
PCR-H683-48D 50T
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Description: An conventional PCR kit for detection of Entamoeba

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PCR-H683-96D 100T
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Description: An conventional PCR kit for detection of Entamoeba

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PCR-H684-48D 50T
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Description: An conventional PCR kit for detection of Acanthamoeba

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Description: An conventional PCR kit for detection of Acanthamoeba

Echovirus PCR kit
PCR-H722-48D 50T
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Description: An conventional PCR kit for detection of Echovirus

Echovirus PCR kit
PCR-H722-96D 100T
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Description: An conventional PCR kit for detection of Echovirus

ranavirus PCR kit
PCR-V337-48D 50T
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Description: An conventional PCR kit for detection of ranavirus

ranavirus PCR kit
PCR-V337-96D 100T
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Description: An conventional PCR kit for detection of ranavirus

Ruminococcus PCR kit
PCR-V619-48D 50T
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Description: An conventional PCR kit for detection of Ruminococcus

Ruminococcus PCR kit
PCR-V619-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Ruminococcus

Bifidobacterium PCR kit
PCR-V621-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Bifidobacterium

Bifidobacterium PCR kit
PCR-V621-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Bifidobacterium

Hantavirus PCR kit
PCR-VH373-48R 50T
EUR 524.4
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Description: An conventional PCR kit for detection of Hantavirus

Hantavirus PCR kit
PCR-VH373-96R 100T
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Description: An conventional PCR kit for detection of Hantavirus

Ruminococcus PCR kit
PCR-H619-48D 48T
EUR 453

Ruminococcus PCR kit
PCR-H619-96D 96T
EUR 572

Bifidobacterium PCR kit
PCR-H621-48D 48T
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Bifidobacterium PCR kit
PCR-H621-96D 96T
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Microthrix parvicella PCR kit
PCR-A575-48D 50T
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Description: An conventional PCR kit for detection of Microthrix parvicella

Microthrix parvicella PCR kit
PCR-A575-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Microthrix parvicella

Yersinia pseudotuberculosis PCR kit
PCR-H190-48D 50T
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Description: An conventional PCR kit for detection of Yersinia pseudotuberculosis

Yersinia pseudotuberculosis PCR kit
PCR-H190-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Yersinia pseudotuberculosis

Neisseria meningitidis PCR kit
PCR-H421-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Neisseria meningitidis