Genetici-Technologies

by Charles Jul 23, 2020 Assay Kits

Best Column Bacterial Genomic DNA Miniprep Kit

Metagenomic water high quality monitoring with a conveyable laboratory

We describe the technical feasibility of metagenomic water high quality evaluation utilizing solely moveable tools, for instance mini-vacuum pumps and filtration models, mini-centrifuges, mini-PCR machines and the memory-stick sized MinION of Oxford Nanopore Applied sciences, for the library preparation and sequencing of 16S rRNA gene amplicons.

1 KB DNA LADDER, 500UL PER KIT

Utilizing this moveable toolbox on website, we efficiently characterised the microbiome of water samples collected from Birtley Sewage Therapy Plant, UK, and its environs. We additionally demonstrated the applicability of the moveable metagenomics toolbox in a low-income nation by surveying water samples from the Akaki River round Addis Ababa, Ethiopia.

The 16S rRNA gene sequencing workflow, together with DNA extraction, PCR amplification, sequencing library preparation, and sequencing was completed inside one working day. The metagenomic knowledge grew to become obtainable inside 24-72 h, relying on web pace.

Bacterial DNA Extraction Kit (Proteinase K included)

Metagenomic evaluation clearly distinguished the microbiome of pristine samples from sewage influenced water samples. Metagenomic evaluation recognized the potential function of two bacterial genera not conventionally monitored, Arcobacter and Aeromonas, as predominant faecal air pollution indicators/waterborne hazards.

 

Genetici-Technologies
Genetici-Technologies

Subsequent quantitative PCR evaluation validated the excessive Arcobacter butzleri abundances noticed within the city influenced Akaki River water samples by moveable subsequent technology sequencing with the MinION system. General, our discipline deployable metagenomics toolbox advances the aptitude of scientists to comprehensively monitor microbiomes wherever on the planet, together with within the water, meals and drinks industries, the well being providers, agriculture and past.

Key phrases: Fecal supply monitoring; Area kits; Metagenomics; MinION nanopore sequencing; Water high quality; Waterborne hazards.

1 KB DNA LADDER, 500UL PER KIT
M-DNA-1KB 1/pk
EUR 70
Description: Bioscience Mol Bio; DNA Ladder

Bacterial DNA Extraction Kit (Proteinase K included)
BA50 50 preps
EUR 100

Bacterial DNA Extraction Kit (Proteinase K included)
BA05 5 preps Ask for price

Bacterial DNA Extraction Kit (Proteinase K included)
BA100 100 preps
EUR 138

Bacterial Flagellin
VAdv-Ly0027 50 µg
EUR 7826
Description: Bacterial flagellin, a TLR5 agonist vaccine adjuvant.

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS423 50Preps
EUR 81.97

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS624 250preps
EUR 193.22

NFNB, bacterial recombinant
7830-100
EUR 403

Collagenase (bacterial) antibody
20C-CR6033RP 500 ul
EUR 430
Description: Rabbit polyclonal Collagenase (bacterial) antibody

Bacterial Recombinant MDH
6357-100
EUR 479

Bacterial Recombinant GOR
6380-100
EUR 457

Bacterial Recombinant SurA
6381-100
EUR 457

Bacterial RNA Extraction Kit
R403-01 100 rxn
EUR 155

ATCC25922 Standard bacterial Strains
S0077 100 ul
EUR 365

ATCC25923 Standard bacterial Strains
S0078 100 ul
EUR 365

ATCC17802 Standard bacterial Strains
S0079 100 ul
EUR 365

ATCC16404 Standard bacterial Strains
S0080 100 ul
EUR 365

ATCC13813 Standard bacterial Strains
S0092 100 ul
EUR 365

ATCC27853 Standard bacterial Strains
S0110 100 ul
EUR 365

ATCC10792 Standard bacterial Strains
S0111 100 ul
EUR 365

ATCC19606 Standard bacterial Strains
S0113 100 ul
EUR 365

EasyRNA? Bacterial RNA Kit
K1351-250
EUR 968

EasyRNA? Bacterial RNA Kit
K1351-50
EUR 370

Bacterial Collagenase Assay Kit
3014 1 kit
EUR 583.6
Description: Bacterial Collagenase Assay Kit

Oxalate Decarboxylase, Active Bacterial Recombinant
7262-100
EUR 446

Oxalate Decarboxylase, Active Bacterial Recombinant
7262-1000
EUR 2931

Oxalate Decarboxylase, Active Bacterial Recombinant
7262-20
EUR 245

Sushi Peptide S3, bacterial recombinant
7611-100
EUR 370

Sushi Peptide S3, bacterial recombinant
7611-1000
EUR 1023

Sushi Peptide S3, bacterial recombinant
7611-20
EUR 153

EZLys? Bacterial Protein Extraction Reagent
8001-100
EUR 131

EZLys? Bacterial Protein Extraction Reagent
8001-500
EUR 294

Bacterial Counting Colorimetric Assay Kit
K511-2500
EUR 620

Bacterial Counting Colorimetric Assay Kit
K511-500
EUR 349

Acetobacter xylinum Standard bacterial Strains
S0090 100 ul
EUR 365

Complete Bacterial Protein Extraction Reagent
AR0157 50mL (sufficient for lysis of 10g of bacterial biomass)
EUR 141

Bacterial Total Protein Extraction Reagent
abx090632-50100assays 50-100 assays
EUR 258

Ricketssia like bacterial PCR kit
PCR-V283-48D 50T
EUR 453

Ricketssia like bacterial PCR kit
PCR-V283-96D 100T
EUR 572

pC001 - huLshC2C2- MBP for bacterial expression
PVT10645 2 ug
EUR 301

CMCC (B) 51572 Standard bacterial Strains
S0081 100 ul
EUR 365

 

Intestine microbial composition adjustments in bladder most cancers sufferers: A case-control research in Harbin, China

Background and goals: This research aimed to discover the adjustments of intestine micro organism in bladder most cancers sufferers.

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

Strategies and research design: Newly recognized bladder most cancers sufferers had been recruited. All members accomplished a questionnaire about private conduct and weight loss program. Pyrosequencing of the overall genomic DNA extracted from human feces was carried out by Illumina HiSeq 2000. The copy variety of goal DNA for micro organism was decided by real-time quantitative PCR assay. Fecal brief chain fatty acids contents had been measured by gasoline chromatography (GC) evaluation. The concentrations of lipopolysaccharide and D-lactic acid in serum had been decided by enzyme-linked immunosorbent assay kits.

Outcomes: Fruit consumption was considerably decrease than in wholesome controls. The numbers of Clostridium cluster XI and Prevotella in bladder most cancers sufferers decreased. The numbers of area micro organism and Prevotella had been considerably and positively related to fruit consumption (r=0.002, p<0.05 for area micro organism; r=0.004, p<0.05 for Prevotella). The focus of butyric acid decreased considerably in bladder most cancers sufferers, and the portions of fecal butyric acid had been considerably and positively related to fruit consumption (r=0.610, p<0.01). The concentrations of lipopolysaccharide and D-lactic acid, two delicate markers of intestine permeability, had been larger in bladder most cancers sufferers.

Conclusions: Dysbiosis of intestine microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity had been present in bladder most cancers sufferers, which may be related to insufficient fruit consumption.

 

Oxalate Decarboxylase, Active Bacterial Recombinant

Background: Shotgun metagenomic sequencing has improved our understanding of the human intestine microbiota. Numerous DNA extraction strategies have been in comparison with discover protocols that robustly and most precisely mirror the unique microbial neighborhood buildings.

Nevertheless, these suggestions will be additional refined by contemplating the time and value calls for in coping with samples from very massive human cohorts. Moreover, fungal DNA extraction efficiency has thus far been little investigated.

Outcomes: We in contrast 6 DNA extraction protocols, MagPure Quick StoolDNA KF Equipment B, Macherey Nagel™ NucleoSpin™®Soil equipment, Zymo Analysis Fast-DNA™ Fecal/SoilMicrobe equipment, MOBIO DNeasy PowerSoil equipment, the guide non-commercial protocol MetaHIT, and the just lately printed protocol Q utilizing 1 microbial mock neighborhood (MMC) (containing eight bacterial and a couple of fungal strains) and fecal samples.

EZLys? Bacterial Protein Extraction Reagent
All samples had been manually extracted and subjected to shotgun metagenomics sequencing. Extracting DNA revealed excessive reproducibility inside all 6 protocols, however microbial extraction efficiencies various. The MMC outcomes demonstrated that bead dimension was a figuring out issue for fungal and bacterial DNA yields. In human fecal samples, the MagPure bacterial extraction carried out in addition to the standardized protocol Q however was quicker and less expensive.

Extraction utilizing the PowerSoil protocol resulted in a considerably greater ratio of gram-negative to gram-positive micro organism than different protocols, which could contribute to reported intestine microbial variations between wholesome adults.

Conclusions: We emphasize the significance of bead dimension choice for bacterial and fungal DNA extraction.

Extra importantly, the efficiency of the novel protocol MP matched that of the really helpful standardized protocol Q however consumed much less time, was less expensive, and is really helpful for additional large-scale human intestine metagenomic research.

Key phrases: DNA extraction; intestine microbiota; human fecal pattern; shotgun metagenomic sequencing.

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-For50mlBlood For 50 ml Blood
EUR 217

Blood Genomic DNA Kit (5-250 ul)
20-abx098078
  • EUR 411.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (No RNase A)
20-abx098864
  • EUR 384.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (with Magnetic Stand)
abx098867-50rxns 50 rxns
EUR 300

Blood Genomic DNA Extraction Mini Kit (100prep)
FABGK-100 100 preps
EUR 165

Blood Genomic DNA Extraction Mini Kit (300prep)
FABGK-300 300 preps
EUR 266

LumiPure genomic DNA Blood and Buccal Kit, 50
21553 50 preps
EUR 97

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92031 2Plates, 192prep
EUR 175.71

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92032 5Plates, 480prep
EUR 324.92

Genomic DNA Kit
20-abx098076
  • EUR 439.00
  • EUR 244.00
  • 200 rxns
  • 50 rxns

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit
SK8253 50Preps
EUR 83.4

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit
SK8254 100preps
EUR 120.72

Blood Genomic DNA Extraction MIni Kit (Automatic Machine) Sample
FABGK-AUTO-024 24 preps
EUR 150

Genomic DNA Isolation Kit
55R-1362 50 assays
EUR 385
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA Extraction Kit
K5016005 1 kit
EUR 329

Genomic DNA Isolation Kit
K281-50
EUR 272

Soil Genomic DNA Kit
K1411-250
EUR 561

Soil Genomic DNA Kit
K1411-50
EUR 316

Insect Genomic DNA Kit
K1412-250
EUR 561

Insect Genomic DNA Kit
K1412-50
EUR 316

Mollusc Genomic DNA Kit
K1413-250
EUR 561

Mollusc Genomic DNA Kit
K1413-50
EUR 316

Yeast Genomic DNA Kit
K1414-250
EUR 561

Yeast Genomic DNA Kit
K1414-50
EUR 316

Fungal Genomic DNA Kit
K1415-250
EUR 561

Fungal Genomic DNA Kit
K1415-50
EUR 316

Plant Genomic DNA Kit
abx294004-50preps 50 preps
EUR 398

Animal Genomic DNA Kit
abx294005-100preps 100 preps
EUR 523

Animal Genomic DNA Kit
abx294005-50preps 50 preps
EUR 398

Plant Genomic DNA Kit
20-abx098077
  • EUR 411.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns

Bacteria Genomic DNA Kit
20-abx098080
  • EUR 105.00
  • EUR 244.00
  • 2 rxns
  • 50 rxns

 

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