Genetici-Technologies

by Charles Jul 23, 2020 Assay Kits

Best Column Bacterial Genomic DNA Miniprep Kit

Metagenomic water high quality monitoring with a conveyable laboratory

We describe the technical feasibility of metagenomic water high quality evaluation utilizing solely moveable tools, for instance mini-vacuum pumps and filtration models, mini-centrifuges, mini-PCR machines and the memory-stick sized MinION of Oxford Nanopore Applied sciences, for the library preparation and sequencing of 16S rRNA gene amplicons.

1 KB DNA LADDER, 500UL PER KIT

Utilizing this moveable toolbox on website, we efficiently characterised the microbiome of water samples collected from Birtley Sewage Therapy Plant, UK, and its environs. We additionally demonstrated the applicability of the moveable metagenomics toolbox in a low-income nation by surveying water samples from the Akaki River round Addis Ababa, Ethiopia.

The 16S rRNA gene sequencing workflow, together with DNA extraction, PCR amplification, sequencing library preparation, and sequencing was completed inside one working day. The metagenomic knowledge grew to become obtainable inside 24-72 h, relying on web pace.

Bacterial DNA Extraction Kit (Proteinase K included)

Metagenomic evaluation clearly distinguished the microbiome of pristine samples from sewage influenced water samples. Metagenomic evaluation recognized the potential function of two bacterial genera not conventionally monitored, Arcobacter and Aeromonas, as predominant faecal air pollution indicators/waterborne hazards.

 

Genetici-Technologies
Genetici-Technologies

Subsequent quantitative PCR evaluation validated the excessive Arcobacter butzleri abundances noticed within the city influenced Akaki River water samples by moveable subsequent technology sequencing with the MinION system. General, our discipline deployable metagenomics toolbox advances the aptitude of scientists to comprehensively monitor microbiomes wherever on the planet, together with within the water, meals and drinks industries, the well being providers, agriculture and past.

Key phrases: Fecal supply monitoring; Area kits; Metagenomics; MinION nanopore sequencing; Water high quality; Waterborne hazards.

Bacterial DNA Purification Kit II
K1457-250 250 Preps
EUR 697.2

Bacterial DNA Purification Kit II
K1457-50 50 Preps
EUR 381.6

EcoSpin Bacterial Genomic DNA Kit
E1050 50 rxn
EUR 53.9
Description: EcoSpin Bacterial Genomic DNA Kit is designed as a simple and convenient purification of high quality genomic DNA from Gram (-) negative and Gram (+) positive bacterial cells. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 25 minutes and purified DNA can be used directly in PCR, qPCR, sequencing and enzymatic reactions.

Milk Bacterial DNA Extraction Kit (50prep)
FAMBD-001 50 preps
EUR 328.8

Milk Bacterial DNA Extraction Kit (50prep)
FAMBD_001 1 kit
EUR 134.5

Bacterial Genomic DNA Isolation Kit
17900 50 Preps
EUR 99

Bacterial Genomic DNA Isolation Kit
K309-100 each
EUR 451.2

Bacterial Genomic DNA Extraction kit
2513A 50 Tests
EUR 80

Bacterial Genomic DNA Extraction kit
2513B 100 Tests
EUR 80

Bacterial Genomic DNA Extraction kit
2513C 2*100 Tests
EUR 150

Bacterial Genomic DNA Extraction kit
2514A 20 Tests
EUR 250

Bacterial Genomic DNA Extraction kit
2514B 50 Tests
EUR 450

GeneMATRIX TISSUE & BACTERIAL DNA Purification Kit
E3551-01 50preps
EUR 98.1
Description: Kit for isolation of total DNA from human and animal tissues and bacteria

GeneMATRIX TISSUE & BACTERIAL DNA Purification Kit
E3551-02 150preps
EUR 275.77
Description: Kit for isolation of total DNA from human and animal tissues and bacteria

Staph A Quantified Bacterial DNA Standard
28301 1 Unit
EUR 395.4

AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria
Z1322006 1 kit (48 extractions) Including all required plastic disposables
EUR 230.78

Bacterial DNA Extraction Kit (Proteinase K included)
BA05 5 preps Ask for price

Bacterial DNA Extraction Kit (Proteinase K included)
BA100 100 preps
EUR 110

Bacterial DNA Extraction Kit (Proteinase K included)
BA50 50 preps
EUR 74

Bacterial DNA Extraction Kit (Proteinase K included)
BA96-05 5x96 preps
EUR 694

Samonella enterica Quantified Bacterial DNA Standard
28300 1 Unit
EUR 395.4

HiPer® Bacterial Genomic DNA Extraction
HTBM008E-10PR 1 unit
EUR 32.63
Description: HiPer® Bacterial Genomic DNA Extraction

HiPer® Bacterial Genomic DNA Extraction
HTBM009E-10PR 1 unit
EUR 37.4
Description: HiPer® Bacterial Genomic DNA Extraction

Neisseria gonorrhoeae Quantified Bacterial DNA Standard
28296 1 Unit
EUR 395.4

Listeria monocytogenes Quantified Bacterial DNA Standard
28299 1 Unit
EUR 395.4

Bacterial Genomic DNA Isolation 96-Well Kit
17950 2 Plates
EUR 299.4

Insta NX® Bacterial Genomic DNA Purifica
MBIN006-12PR 1 unit
EUR 71.73
Description: Insta NX® Bacterial Genomic DNA Purifica

SaMag Bacterial DNA Extraction kit For use with SaMag-12/24 instruments; extraction of bacterial DNA of from clinical specimens or coltures
SM006 48
EUR 187.48

HiPurA® Bacterial Genomic DNA Miniprep P
MB505-20PR 1 unit
EUR 38.72
Description: HiPurA® Bacterial Genomic DNA Miniprep P

HiPurA® Bacterial Genomic DNA Miniprep P
MB505-250PR 1 unit
EUR 415.6
Description: HiPurA® Bacterial Genomic DNA Miniprep P

HiPurA® Bacterial Genomic DNA Miniprep P
MB505-50PR 1 unit
EUR 91.51
Description: HiPurA® Bacterial Genomic DNA Miniprep P

Column-Pure Bacterial Genomic DNA Isolation Kit
D511 50 Preparations
EUR 195

HiPurA® 96-Bacterial Genomic DNA Purific
MB548-1X96PR 1 unit
EUR 172.52
Description: HiPurA® 96-Bacterial Genomic DNA Purific

Synovial Fluid Bacterial Genomic DNA Isolation Kit
67900 50 Preps
EUR 203.4

Bacterial Genomic DNA Purification kit（Spin Column)
2512A 50 Tests
EUR 100

Bacterial Genomic DNA Purification kit（Spin Column)
2512B 100 Tests
EUR 180

Bacterial Genomic DNA Purification kit（Spin Column)
2512C 2*100 Tests
EUR 300

GeneMATRIX BACTERIAL & YEAST GENOMIC DNA Purification Kit
E3580-01 50preps
EUR 95.92

GeneMATRIX BACTERIAL & YEAST GENOMIC DNA Purification Kit
E3580-02 150preps
EUR 270.32

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS624 250preps
EUR 231.86

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS423 50Preps
EUR 98.36

 

Intestine microbial composition adjustments in bladder most cancers sufferers: A case-control research in Harbin, China

Background and goals: This research aimed to discover the adjustments of intestine micro organism in bladder most cancers sufferers.

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

Strategies and research design: Newly recognized bladder most cancers sufferers had been recruited. All members accomplished a questionnaire about private conduct and weight loss program. Pyrosequencing of the overall genomic DNA extracted from human feces was carried out by Illumina HiSeq 2000. The copy variety of goal DNA for micro organism was decided by real-time quantitative PCR assay. Fecal brief chain fatty acids contents had been measured by gasoline chromatography (GC) evaluation. The concentrations of lipopolysaccharide and D-lactic acid in serum had been decided by enzyme-linked immunosorbent assay kits.

Outcomes: Fruit consumption was considerably decrease than in wholesome controls. The numbers of Clostridium cluster XI and Prevotella in bladder most cancers sufferers decreased. The numbers of area micro organism and Prevotella had been considerably and positively related to fruit consumption (r=0.002, p<0.05 for area micro organism; r=0.004, p<0.05 for Prevotella). The focus of butyric acid decreased considerably in bladder most cancers sufferers, and the portions of fecal butyric acid had been considerably and positively related to fruit consumption (r=0.610, p<0.01). The concentrations of lipopolysaccharide and D-lactic acid, two delicate markers of intestine permeability, had been larger in bladder most cancers sufferers.

Conclusions: Dysbiosis of intestine microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity had been present in bladder most cancers sufferers, which may be related to insufficient fruit consumption.

 

Oxalate Decarboxylase, Active Bacterial Recombinant

Background: Shotgun metagenomic sequencing has improved our understanding of the human intestine microbiota. Numerous DNA extraction strategies have been in comparison with discover protocols that robustly and most precisely mirror the unique microbial neighborhood buildings.

Nevertheless, these suggestions will be additional refined by contemplating the time and value calls for in coping with samples from very massive human cohorts. Moreover, fungal DNA extraction efficiency has thus far been little investigated.

Outcomes: We in contrast 6 DNA extraction protocols, MagPure Quick StoolDNA KF Equipment B, Macherey Nagel™ NucleoSpin™®Soil equipment, Zymo Analysis Fast-DNA™ Fecal/SoilMicrobe equipment, MOBIO DNeasy PowerSoil equipment, the guide non-commercial protocol MetaHIT, and the just lately printed protocol Q utilizing 1 microbial mock neighborhood (MMC) (containing eight bacterial and a couple of fungal strains) and fecal samples.

EZLys? Bacterial Protein Extraction Reagent
All samples had been manually extracted and subjected to shotgun metagenomics sequencing. Extracting DNA revealed excessive reproducibility inside all 6 protocols, however microbial extraction efficiencies various. The MMC outcomes demonstrated that bead dimension was a figuring out issue for fungal and bacterial DNA yields. In human fecal samples, the MagPure bacterial extraction carried out in addition to the standardized protocol Q however was quicker and less expensive.

Extraction utilizing the PowerSoil protocol resulted in a considerably greater ratio of gram-negative to gram-positive micro organism than different protocols, which could contribute to reported intestine microbial variations between wholesome adults.

Conclusions: We emphasize the significance of bead dimension choice for bacterial and fungal DNA extraction.

Extra importantly, the efficiency of the novel protocol MP matched that of the really helpful standardized protocol Q however consumed much less time, was less expensive, and is really helpful for additional large-scale human intestine metagenomic research.

Key phrases: DNA extraction; intestine microbiota; human fecal pattern; shotgun metagenomic sequencing.

EcoSpin Blood Genomic DNA Kit
EcoBGD-50x 50 rxn
EUR 53.9
Description: EcoSpin Blood Genomic DNA Kit is is designed as a simple and convenient purification of high quality genomic DNA from whole blood. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 25 minutes and purified DNA can be used directly in PCR, qPCR, Southern blotting and enzymatic reactions.

Pig Blood Genomic DNA
PG-705 0.1mg
EUR 223

Blood Genomic DNA Midi
GGD2001 20 preps
EUR 203.16

Blood Genomic DNA Midi
GGD2002 50 preps
EUR 457.11

Blood Genomic DNA Maxi
GGM2001 10 preps
EUR 203.16

Blood Genomic DNA Maxi
GGM2002 25 preps
EUR 457.11

Human Blood Genomic DNA
HG-705 0.05mg
EUR 319

Bovine Blood Genomic DNA
BG-705 0.1mg
EUR 223

Mbead Blood/Cell Genomic DNA Kit
PDM06-0100 100 rxns
EUR 154

Blood Genomic DNA Kit (5-250 µl)
abx098078-100l 100 µl
EUR 287.5

Blood Genomic DNA Kit (5-250 µl)
abx098078-1ml 1 ml Ask for price

Blood Genomic DNA Kit (5-250 µl)
abx098078-200l 200 µl
EUR 512.5

Blood Genomic DNA Kit (5-250 ul)
20-abx098078
  • Ask for price
  • Ask for price
  • 50 rxns
  • 200 rxns

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-100l 100 µl
EUR 275

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-1ml 1 ml Ask for price

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-200l 200 µl
EUR 437.5

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-For200mlBlood For 200 ml Blood
EUR 427.2

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-For50mlBlood For 50 ml Blood
EUR 260.4

Column-Pure Blood Genomic DNA Kit
D203-Mini-1 50 Preparations
EUR 105

Blood Genomic DNA Kit (No RNase A)
abx098864-100l 100 µl
EUR 287.5

Blood Genomic DNA Kit (No RNase A)
abx098864-1ml 1 ml Ask for price

Blood Genomic DNA Kit (No RNase A)
abx098864-200l 200 µl
EUR 475

Blood Genomic DNA Kit (No RNase A)
20-abx098864
  • Ask for price
  • Ask for price
  • 50 rxns
  • 200 rxns

Rapid Blood Genomic DNA Extraction Kit
BT4782 50Preps
EUR 91.97

Whool Blood Genomic DNA Purification Kit
2503A 50*0.3 mL
EUR 50

Whool Blood Genomic DNA Purification Kit
2503B 100*0.3 mL
EUR 80

Whool Blood Genomic DNA Purification Kit
2503C 200*0.3 mL
EUR 100

Whool Blood Genomic DNA Purification Kit
2504A 20*3 mL
EUR 100

Whool Blood Genomic DNA Purification Kit
2504B 50*3 mL
EUR 200

Whool Blood Genomic DNA Purification Kit
2505A 16*10 mL
EUR 200

Whool Blood Genomic DNA Purification Kit
2505B 32*10 mL
EUR 300

 

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