Genetici-Technologies

Jul 23, 2020 Assay Kits

Best Column Bacterial Genomic DNA Miniprep Kit

Metagenomic water high quality monitoring with a conveyable laboratory

We describe the technical feasibility of metagenomic water high quality evaluation utilizing solely moveable tools, for instance mini-vacuum pumps and filtration models, mini-centrifuges, mini-PCR machines and the memory-stick sized MinION of Oxford Nanopore Applied sciences, for the library preparation and sequencing of 16S rRNA gene amplicons.

1 KB DNA LADDER, 500UL PER KIT

Utilizing this moveable toolbox on website, we efficiently characterised the microbiome of water samples collected from Birtley Sewage Therapy Plant, UK, and its environs. We additionally demonstrated the applicability of the moveable metagenomics toolbox in a low-income nation by surveying water samples from the Akaki River round Addis Ababa, Ethiopia.

The 16S rRNA gene sequencing workflow, together with DNA extraction, PCR amplification, sequencing library preparation, and sequencing was completed inside one working day. The metagenomic knowledge grew to become obtainable inside 24-72 h, relying on web pace.

Bacterial DNA Extraction Kit (Proteinase K included)

Metagenomic evaluation clearly distinguished the microbiome of pristine samples from sewage influenced water samples. Metagenomic evaluation recognized the potential function of two bacterial genera not conventionally monitored, Arcobacter and Aeromonas, as predominant faecal air pollution indicators/waterborne hazards.

 

Genetici-Technologies
Genetici-Technologies

Subsequent quantitative PCR evaluation validated the excessive Arcobacter butzleri abundances noticed within the city influenced Akaki River water samples by moveable subsequent technology sequencing with the MinION system. General, our discipline deployable metagenomics toolbox advances the aptitude of scientists to comprehensively monitor microbiomes wherever on the planet, together with within the water, meals and drinks industries, the well being providers, agriculture and past.

Key phrases: Fecal supply monitoring; Area kits; Metagenomics; MinION nanopore sequencing; Water high quality; Waterborne hazards.

AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria

Z1322006 1 kit (48 extractions) Including all required plastic disposables
EUR 259

Bacterial DNA Extraction Kit (Proteinase K included)

BA50 50 preps
EUR 120

Bacterial DNA Extraction Kit (Proteinase K included)

BA05 5 preps Ask for price

Bacterial DNA Extraction Kit (Proteinase K included)

BA100 100 preps
EUR 165.6

Bacterial DNA Extraction Kit (Proteinase K included)

BA96-05 5x96 preps
EUR 694

HiPer® Bacterial Genomic DNA Extraction

HTBM008E-10PR 1 unit
EUR 32.63
Description: HiPer® Bacterial Genomic DNA Extraction

HiPer® Bacterial Genomic DNA Extraction

HTBM009E-10PR 1 unit
EUR 37.4
Description: HiPer® Bacterial Genomic DNA Extraction

Insta NX® Bacterial Genomic DNA Purifica

MBIN006-12PR 1 unit
EUR 71.73
Description: Insta NX® Bacterial Genomic DNA Purifica

HiPurA® Bacterial Genomic DNA Miniprep P

MB505-20PR 1 unit
EUR 38.72
Description: HiPurA® Bacterial Genomic DNA Miniprep P

HiPurA® Bacterial Genomic DNA Miniprep P

MB505-250PR 1 unit
EUR 415.6
Description: HiPurA® Bacterial Genomic DNA Miniprep P

HiPurA® Bacterial Genomic DNA Miniprep P

MB505-50PR 1 unit
EUR 91.51
Description: HiPurA® Bacterial Genomic DNA Miniprep P

Column-Pure Bacterial Genomic DNA Isolation Kit

D511 50 Preparations
EUR 195

HiPurA® 96-Bacterial Genomic DNA Purific

MB548-1X96PR 1 unit
EUR 172.52
Description: HiPurA® 96-Bacterial Genomic DNA Purific

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS423 50Preps
EUR 98.36

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS624 250preps
EUR 231.86

GF-1 Bacterial Starter Kit / Taq DNA Polymerase, 25 preps

GF-BA-K each Ask for price

ExoQuali™ Bacterial MVs Isolation Kit (bacterial culture media)

CEIK-092L-10T 10 T Ask for price

ExoQuali™ Bacterial MVs Isolation Kit (bacterial culture media)

CEIK-092L-20T 20 T Ask for price

GF-1 Bacterial DNA Extraction Kit (Proteinase K included), 50 preps

GF-BA-050 each Ask for price

GF-1 Bacterial DNA Extraction Kit (Proteinase K included), 100 preps

GF-BA-100 each Ask for price

Bacterial Flagellin

VAdv-Ly0027 50 µg
EUR 9391.2
Description: Bacterial flagellin, a TLR5 agonist vaccine adjuvant.

GF-1 Bacterial Starter Kit / Chromo Taq DNA Polymerase, 25 preps

GF-BA-KW each Ask for price

EasyRNA? Bacterial RNA Kit

K1351-250 each
EUR 1161.6

EasyRNA? Bacterial RNA Kit

K1351-50 each
EUR 444

Bacterial Transformation Kit

MBT127-10R 1 unit
EUR 18.24
Description: Bacterial Transformation Kit

Bacterial Transformation Kit

MBT127-20R 1 unit
EUR 32.87
Description: Bacterial Transformation Kit

Bacterial Transformation Kit

MBT127-40R 1 unit
EUR 58.64
Description: Bacterial Transformation Kit

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 186

Bacterial Recombinant MDH

6357-100 each
EUR 574.8

Bacterial Recombinant GOR

6380-100 each
EUR 548.4

Ricketssia like bacterial

Oneq-V283-OneqV283100D Oneq-V283-100D
EUR 515

Ricketssia like bacterial

Oneq-V283-OneqV283150D Oneq-V283-150D
EUR 594

Ricketssia like bacterial

Oneq-V283-OneqV28350D Oneq-V283-50D
EUR 413

Ricketssia like bacterial

PCR-V283-PCRV28348D PCR-V283-48D
EUR 230

Ricketssia like bacterial

PCR-V283-PCRV28396D PCR-V283-96D
EUR 312

NFNB, bacterial recombinant

7830-100 each
EUR 483.6

Bacterial Recombinant SurA

6381-100 each
EUR 548.4

Bacterial Sialidase Antibody

GWB-9CC345 1 ml Ask for price

Bacterial Collagenase Assay Kit

3014 1 kit
EUR 409
Description: Culture Media, Tissue Homogenate

Collagenase (bacterial) antibody

20C-CR6033RP 500 ul
EUR 337
Description: Rabbit polyclonal Collagenase (bacterial) antibody

genesigPLEX GI Bacterial Kit 2

R01046 100 tests
EUR 1777

 

Intestine microbial composition adjustments in bladder most cancers sufferers: A case-control research in Harbin, China

Background and goals: This research aimed to discover the adjustments of intestine micro organism in bladder most cancers sufferers.

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

Strategies and research design: Newly recognized bladder most cancers sufferers had been recruited. All members accomplished a questionnaire about private conduct and weight loss program. Pyrosequencing of the overall genomic DNA extracted from human feces was carried out by Illumina HiSeq 2000. The copy variety of goal DNA for micro organism was decided by real-time quantitative PCR assay. Fecal brief chain fatty acids contents had been measured by gasoline chromatography (GC) evaluation. The concentrations of lipopolysaccharide and D-lactic acid in serum had been decided by enzyme-linked immunosorbent assay kits.

Outcomes: Fruit consumption was considerably decrease than in wholesome controls. The numbers of Clostridium cluster XI and Prevotella in bladder most cancers sufferers decreased. The numbers of area micro organism and Prevotella had been considerably and positively related to fruit consumption (r=0.002, p<0.05 for area micro organism; r=0.004, p<0.05 for Prevotella). The focus of butyric acid decreased considerably in bladder most cancers sufferers, and the portions of fecal butyric acid had been considerably and positively related to fruit consumption (r=0.610, p<0.01). The concentrations of lipopolysaccharide and D-lactic acid, two delicate markers of intestine permeability, had been larger in bladder most cancers sufferers.

Conclusions: Dysbiosis of intestine microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity had been present in bladder most cancers sufferers, which may be related to insufficient fruit consumption.

 

Oxalate Decarboxylase, Active Bacterial Recombinant

Background: Shotgun metagenomic sequencing has improved our understanding of the human intestine microbiota. Numerous DNA extraction strategies have been in comparison with discover protocols that robustly and most precisely mirror the unique microbial neighborhood buildings.

Nevertheless, these suggestions will be additional refined by contemplating the time and value calls for in coping with samples from very massive human cohorts. Moreover, fungal DNA extraction efficiency has thus far been little investigated.

Outcomes: We in contrast 6 DNA extraction protocols, MagPure Quick StoolDNA KF Equipment B, Macherey Nagel™ NucleoSpin™®Soil equipment, Zymo Analysis Fast-DNA™ Fecal/SoilMicrobe equipment, MOBIO DNeasy PowerSoil equipment, the guide non-commercial protocol MetaHIT, and the just lately printed protocol Q utilizing 1 microbial mock neighborhood (MMC) (containing eight bacterial and a couple of fungal strains) and fecal samples.

EZLys? Bacterial Protein Extraction Reagent

All samples had been manually extracted and subjected to shotgun metagenomics sequencing. Extracting DNA revealed excessive reproducibility inside all 6 protocols, however microbial extraction efficiencies various. The MMC outcomes demonstrated that bead dimension was a figuring out issue for fungal and bacterial DNA yields. In human fecal samples, the MagPure bacterial extraction carried out in addition to the standardized protocol Q however was quicker and less expensive.

Extraction utilizing the PowerSoil protocol resulted in a considerably greater ratio of gram-negative to gram-positive micro organism than different protocols, which could contribute to reported intestine microbial variations between wholesome adults.

Conclusions: We emphasize the significance of bead dimension choice for bacterial and fungal DNA extraction.

Extra importantly, the efficiency of the novel protocol MP matched that of the really helpful standardized protocol Q however consumed much less time, was less expensive, and is really helpful for additional large-scale human intestine metagenomic research.

Key phrases: DNA extraction; intestine microbiota; human fecal pattern; shotgun metagenomic sequencing.

Pig Blood Genomic DNA

PG-705 0.1mg
EUR 223

Human Blood Genomic DNA

HG-705 0.05mg
EUR 319

Bovine Blood Genomic DNA

BG-705 0.1mg
EUR 223

Blood Genomic DNA Kit (5-250 ul)

20-abx098078
  • Ask for price
  • Ask for price
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (5-250 µl)

abx098078-100l 100 µl
EUR 287.5

Blood Genomic DNA Kit (5-250 µl)

abx098078-1ml 1 ml Ask for price

Blood Genomic DNA Kit (5-250 µl)

abx098078-200l 200 µl
EUR 512.5

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For200mlBlood For 200 ml Blood
EUR 427.2

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For50mlBlood For 50 ml Blood
EUR 260.4

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-100l 100 µl
EUR 275

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-1ml 1 ml Ask for price

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-200l 200 µl
EUR 437.5

Column-Pure Blood Genomic DNA Kit

D203-Mini-1 50 Preparations
EUR 105

Blood Genomic DNA Kit (No RNase A)

20-abx098864
  • Ask for price
  • Ask for price
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (No RNase A)

abx098864-100l 100 µl
EUR 287.5

Blood Genomic DNA Kit (No RNase A)

abx098864-1ml 1 ml Ask for price

Blood Genomic DNA Kit (No RNase A)

abx098864-200l 200 µl
EUR 475

Rapid Blood Genomic DNA Extraction Kit

BT4782 50Preps
EUR 91.97

Magnetic bead Blood/Cell Genomic DNA Kit

PDM06-0100 100 rcs
EUR 265

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-50rxns 50 rxns
EUR 360

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-100l 100 µl
EUR 375

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-1ml 1 ml Ask for price

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-200l 200 µl Ask for price

Magnetic Beads-Based Blood Genomic DNA Kit

abx460020-1096tests 10 × 96 tests Ask for price

Magnetic Beads-Based Blood Genomic DNA Kit

abx460020-596tests 5 × 96 tests Ask for price

Magnetic Beads-Based Blood Genomic DNA Kit

abx460020-96tests 96 tests
EUR 437.5

Genomic DNA Mini Kit (Blood/Cultured Cell)

GB004 each
EUR 3

Genomic DNA Mini Kit (Blood/Cultured Cell)

GB100 each
EUR 70

Genomic DNA Mini Kit (Blood/Cultured Cell)

GB300 each
EUR 189

Genomic DNA Maxi Kit (Blood/Cultured Cell)

GDM002 each
EUR 21

Genomic DNA Maxi Kit (Blood/Cultured Cell)

GDM010 each
EUR 89.3

 

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