Genetici-Technologies

by Charles Jul 23, 2020 Assay Kits

Best Column Bacterial Genomic DNA Miniprep Kit

Metagenomic water high quality monitoring with a conveyable laboratory

We describe the technical feasibility of metagenomic water high quality evaluation utilizing solely moveable tools, for instance mini-vacuum pumps and filtration models, mini-centrifuges, mini-PCR machines and the memory-stick sized MinION of Oxford Nanopore Applied sciences, for the library preparation and sequencing of 16S rRNA gene amplicons.

1 KB DNA LADDER, 500UL PER KIT

Utilizing this moveable toolbox on website, we efficiently characterised the microbiome of water samples collected from Birtley Sewage Therapy Plant, UK, and its environs. We additionally demonstrated the applicability of the moveable metagenomics toolbox in a low-income nation by surveying water samples from the Akaki River round Addis Ababa, Ethiopia.

The 16S rRNA gene sequencing workflow, together with DNA extraction, PCR amplification, sequencing library preparation, and sequencing was completed inside one working day. The metagenomic knowledge grew to become obtainable inside 24-72 h, relying on web pace.

Bacterial DNA Extraction Kit (Proteinase K included)

Metagenomic evaluation clearly distinguished the microbiome of pristine samples from sewage influenced water samples. Metagenomic evaluation recognized the potential function of two bacterial genera not conventionally monitored, Arcobacter and Aeromonas, as predominant faecal air pollution indicators/waterborne hazards.

 

Genetici-Technologies
Genetici-Technologies

Subsequent quantitative PCR evaluation validated the excessive Arcobacter butzleri abundances noticed within the city influenced Akaki River water samples by moveable subsequent technology sequencing with the MinION system. General, our discipline deployable metagenomics toolbox advances the aptitude of scientists to comprehensively monitor microbiomes wherever on the planet, together with within the water, meals and drinks industries, the well being providers, agriculture and past.

Key phrases: Fecal supply monitoring; Area kits; Metagenomics; MinION nanopore sequencing; Water high quality; Waterborne hazards.

100 BP DNA LADDER, 500UL PER KIT
M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT
M-DNA-1KB 1/pk
EUR 84
Description: Bioscience Mol Bio; DNA Ladder

Bacterial DNA Extraction Kit (Proteinase K included)
BA05 5 preps Ask for price

Bacterial DNA Extraction Kit (Proteinase K included)
BA100 100 preps
EUR 165.6

Bacterial DNA Extraction Kit (Proteinase K included)
BA50 50 preps
EUR 120

Bacterial Flagellin
VAdv-Ly0027 50 µg
EUR 9391.2
Description: Bacterial flagellin, a TLR5 agonist vaccine adjuvant.

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS423 50Preps
EUR 98.36

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS624 250preps
EUR 231.86

Bacterial Recombinant MDH
6357-100
EUR 574.8

Bacterial Recombinant GOR
6380-100
EUR 548.4

Bacterial Recombinant SurA
6381-100
EUR 548.4

Collagenase (bacterial) antibody
20C-CR6033RP 500 ul
EUR 516
Description: Rabbit polyclonal Collagenase (bacterial) antibody

NFNB, bacterial recombinant
7830-100
EUR 483.6

Bacterial Collagenase Assay Kit
3014 1 kit
EUR 700.32
Description: Bacterial Collagenase Assay Kit

EasyRNA? Bacterial RNA Kit
K1351-250
EUR 1161.6

EasyRNA? Bacterial RNA Kit
K1351-50
EUR 444

Bacterial RNA Extraction Kit
R403-01 100 rxn
EUR 186

ATCC25922 Standard bacterial Strains
S0077 100 ul
EUR 438

ATCC25923 Standard bacterial Strains
S0078 100 ul
EUR 438

ATCC17802 Standard bacterial Strains
S0079 100 ul
EUR 438

ATCC16404 Standard bacterial Strains
S0080 100 ul
EUR 438

ATCC13813 Standard bacterial Strains
S0092 100 ul
EUR 438

ATCC27853 Standard bacterial Strains
S0110 100 ul
EUR 438

ATCC10792 Standard bacterial Strains
S0111 100 ul
EUR 438

ATCC19606 Standard bacterial Strains
S0113 100 ul
EUR 438

Agar Bacterial Tech 2Kg
281210 EACH
EUR 571.14

Oxalate Decarboxylase, Active Bacterial Recombinant
7262-100
EUR 535.2

Oxalate Decarboxylase, Active Bacterial Recombinant
7262-1000
EUR 3517.2

Oxalate Decarboxylase, Active Bacterial Recombinant
7262-20
EUR 294

Bacterial Total Protein Extraction Reagent
abx090632-50100assays 50-100 assays
EUR 309.6

EZLys? Bacterial Protein Extraction Reagent
8001-100
EUR 157.2

EZLys? Bacterial Protein Extraction Reagent
8001-500
EUR 352.8

Sushi Peptide S3, bacterial recombinant
7611-100
EUR 444

Sushi Peptide S3, bacterial recombinant
7611-1000
EUR 1227.6

Sushi Peptide S3, bacterial recombinant
7611-20
EUR 183.6

Complete Bacterial Protein Extraction Reagent
AR0157 50mL (sufficient for lysis of 10g of bacterial biomass)
EUR 169.2

Bacterial Counting Colorimetric Assay Kit
K511-2500
EUR 744

Bacterial Counting Colorimetric Assay Kit
K511-500
EUR 418.8

Ricketssia like bacterial PCR kit
PCR-V283-48D 50T
EUR 543.6

Ricketssia like bacterial PCR kit
PCR-V283-96D 100T
EUR 686.4

Acetobacter xylinum Standard bacterial Strains
S0090 100 ul
EUR 438

 

Intestine microbial composition adjustments in bladder most cancers sufferers: A case-control research in Harbin, China

Background and goals: This research aimed to discover the adjustments of intestine micro organism in bladder most cancers sufferers.

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

Strategies and research design: Newly recognized bladder most cancers sufferers had been recruited. All members accomplished a questionnaire about private conduct and weight loss program. Pyrosequencing of the overall genomic DNA extracted from human feces was carried out by Illumina HiSeq 2000. The copy variety of goal DNA for micro organism was decided by real-time quantitative PCR assay. Fecal brief chain fatty acids contents had been measured by gasoline chromatography (GC) evaluation. The concentrations of lipopolysaccharide and D-lactic acid in serum had been decided by enzyme-linked immunosorbent assay kits.

Outcomes: Fruit consumption was considerably decrease than in wholesome controls. The numbers of Clostridium cluster XI and Prevotella in bladder most cancers sufferers decreased. The numbers of area micro organism and Prevotella had been considerably and positively related to fruit consumption (r=0.002, p<0.05 for area micro organism; r=0.004, p<0.05 for Prevotella). The focus of butyric acid decreased considerably in bladder most cancers sufferers, and the portions of fecal butyric acid had been considerably and positively related to fruit consumption (r=0.610, p<0.01). The concentrations of lipopolysaccharide and D-lactic acid, two delicate markers of intestine permeability, had been larger in bladder most cancers sufferers.

Conclusions: Dysbiosis of intestine microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity had been present in bladder most cancers sufferers, which may be related to insufficient fruit consumption.

 

Oxalate Decarboxylase, Active Bacterial Recombinant

Background: Shotgun metagenomic sequencing has improved our understanding of the human intestine microbiota. Numerous DNA extraction strategies have been in comparison with discover protocols that robustly and most precisely mirror the unique microbial neighborhood buildings.

Nevertheless, these suggestions will be additional refined by contemplating the time and value calls for in coping with samples from very massive human cohorts. Moreover, fungal DNA extraction efficiency has thus far been little investigated.

Outcomes: We in contrast 6 DNA extraction protocols, MagPure Quick StoolDNA KF Equipment B, Macherey Nagel™ NucleoSpin™®Soil equipment, Zymo Analysis Fast-DNA™ Fecal/SoilMicrobe equipment, MOBIO DNeasy PowerSoil equipment, the guide non-commercial protocol MetaHIT, and the just lately printed protocol Q utilizing 1 microbial mock neighborhood (MMC) (containing eight bacterial and a couple of fungal strains) and fecal samples.

EZLys? Bacterial Protein Extraction Reagent
All samples had been manually extracted and subjected to shotgun metagenomics sequencing. Extracting DNA revealed excessive reproducibility inside all 6 protocols, however microbial extraction efficiencies various. The MMC outcomes demonstrated that bead dimension was a figuring out issue for fungal and bacterial DNA yields. In human fecal samples, the MagPure bacterial extraction carried out in addition to the standardized protocol Q however was quicker and less expensive.

Extraction utilizing the PowerSoil protocol resulted in a considerably greater ratio of gram-negative to gram-positive micro organism than different protocols, which could contribute to reported intestine microbial variations between wholesome adults.

Conclusions: We emphasize the significance of bead dimension choice for bacterial and fungal DNA extraction.

Extra importantly, the efficiency of the novel protocol MP matched that of the really helpful standardized protocol Q however consumed much less time, was less expensive, and is really helpful for additional large-scale human intestine metagenomic research.

Key phrases: DNA extraction; intestine microbiota; human fecal pattern; shotgun metagenomic sequencing.

Blood Genomic DNA Kit (No RNase A)
20-abx098864
  • EUR 460.80
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (with Magnetic Stand)
abx098867-50rxns 50 rxns
EUR 360

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-For200mlBlood For 200 ml Blood
EUR 427.2

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-For50mlBlood For 50 ml Blood
EUR 260.4

Blood Genomic DNA Kit (5-250 ul)
20-abx098078
  • EUR 493.20
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Blood genomic DNA extraction and purification kit
K1443-100
EUR 574.8

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92031 2Plates, 192prep
EUR 210.85

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92032 5Plates, 480prep
EUR 389.9

LumiPure genomic DNA Blood and Buccal Kit, 50
21553 50 preps
EUR 116.4

Genomic DNA Kit
20-abx098076
  • EUR 526.80
  • EUR 292.80
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Extraction MIni Kit (Automatic Machine) Sample
FABGK-AUTO-024 24 preps
EUR 180

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit
SK8253 50Preps
EUR 100.08

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit
SK8254 100preps
EUR 144.86

Genomic DNA Isolation Kit
55R-1362 50 assays
EUR 462
Description: Genomic DNA Isolation Kit for use in the research laboratory

Plant Genomic DNA Kit
20-abx098077
  • EUR 493.20
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Bacteria Genomic DNA Kit
20-abx098080
  • EUR 126.00
  • EUR 292.80
  • 2 rxns
  • 50 rxns

Micro Genomic DNA Kit
20-abx098242
  • EUR 126.00
  • EUR 393.60
  • 2 rxns
  • 50 rxns

Plant Genomic DNA Kit
abx294004-50preps 50 preps
EUR 477.6

Animal Genomic DNA Kit
abx294005-100preps 100 preps
EUR 627.6

Animal Genomic DNA Kit
abx294005-50preps 50 preps
EUR 477.6

Genomic DNA Isolation Kit
K281-50
EUR 326.4

Soil Genomic DNA Kit
K1411-250
EUR 673.2

Soil Genomic DNA Kit
K1411-50
EUR 379.2

Insect Genomic DNA Kit
K1412-250
EUR 673.2

Insect Genomic DNA Kit
K1412-50
EUR 379.2

Mollusc Genomic DNA Kit
K1413-250
EUR 673.2

Mollusc Genomic DNA Kit
K1413-50
EUR 379.2

Yeast Genomic DNA Kit
K1414-250
EUR 673.2

Yeast Genomic DNA Kit
K1414-50
EUR 379.2

Fungal Genomic DNA Kit
K1415-250
EUR 673.2

Fungal Genomic DNA Kit
K1415-50
EUR 379.2

 

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