by Charles Jul 23, 2020 Assay Kits

Best Column Bacterial Genomic DNA Miniprep Kit

Metagenomic water high quality monitoring with a conveyable laboratory

We describe the technical feasibility of metagenomic water high quality evaluation utilizing solely moveable tools, for instance mini-vacuum pumps and filtration models, mini-centrifuges, mini-PCR machines and the memory-stick sized MinION of Oxford Nanopore Applied sciences, for the library preparation and sequencing of 16S rRNA gene amplicons.

1 KB DNA LADDER, 500UL PER KIT

Utilizing this moveable toolbox on website, we efficiently characterised the microbiome of water samples collected from Birtley Sewage Therapy Plant, UK, and its environs. We additionally demonstrated the applicability of the moveable metagenomics toolbox in a low-income nation by surveying water samples from the Akaki River round Addis Ababa, Ethiopia.

The 16S rRNA gene sequencing workflow, together with DNA extraction, PCR amplification, sequencing library preparation, and sequencing was completed inside one working day. The metagenomic knowledge grew to become obtainable inside 24-72 h, relying on web pace.

Bacterial DNA Extraction Kit (Proteinase K included)

Metagenomic evaluation clearly distinguished the microbiome of pristine samples from sewage influenced water samples. Metagenomic evaluation recognized the potential function of two bacterial genera not conventionally monitored, Arcobacter and Aeromonas, as predominant faecal air pollution indicators/waterborne hazards.

Subsequent quantitative PCR evaluation validated the excessive Arcobacter butzleri abundances noticed within the city influenced Akaki River water samples by moveable subsequent technology sequencing with the MinION system. General, our discipline deployable metagenomics toolbox advances the aptitude of scientists to comprehensively monitor microbiomes wherever on the planet, together with within the water, meals and drinks industries, the well being providers, agriculture and past.

Key phrases: Fecal supply monitoring; Area kits; Metagenomics; MinION nanopore sequencing; Water high quality; Waterborne hazards.

Bacterial DNA Extraction Kit (Proteinase K included)

BA50 GeneOn 50 preps
EUR 120

AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria

Z1322006 Biochain 1 kit (48 extractions) Including all required plastic disposables
EUR 216

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS423 Bio Basic 50Preps
EUR 98.36

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS624 Bio Basic 250preps
EUR 231.86

Bacterial Flagellin

VAdv-Ly0027 Creative Biolabs 50 µg
EUR 9391.2

Description: Bacterial flagellin, a TLR5 agonist vaccine adjuvant.

EasyRNA? Bacterial RNA Kit

K1351-250 Biovision each
EUR 1161.6

EasyRNA? Bacterial RNA Kit

K1351-50 Biovision each
EUR 444

Bacterial Recombinant MDH

6357-100 Biovision each
EUR 574.8

Bacterial Recombinant GOR

6380-100 Biovision each
EUR 548.4

Bacterial Recombinant SurA

6381-100 Biovision each
EUR 548.4

NFNB, bacterial recombinant

7830-100 Biovision each
EUR 483.6

Agar Bacterial Tech 2Kg

281210 Scientific Laboratory Supplies EACH
EUR 686.4

Bacterial RNA Extraction Kit

R403-01 Vazyme 100 rxn
EUR 186

Collagenase (bacterial) antibody

20C-CR6033RP Fitzgerald 500 ul
EUR 516

Description: Rabbit polyclonal Collagenase (bacterial) antibody

Bacterial Collagenase Assay Kit

3014 Chondrex 1 kit
EUR 700.32

Description: Bacterial Collagenase Assay Kit

Ricketssia like bacterial PCR kit

PCR-V283-48D Bioingentech 50T
EUR 543.6

Ricketssia like bacterial PCR kit

PCR-V283-96D Bioingentech 100T
EUR 686.4

Bacterial Protein Extraction Buffer

20010 BPS Bioscience 200 ml
EUR 130

Description: BPS Bacterial Protein extraction Buffer enables extraction of both soluble and insoluble (inclusion bodies) proteins from bacteria. The buffer provides high efficiency recovery of over-expressed proteins and is fully compatible with HIS/GST-tag purification procedures and standard protein concentration determination assays. The buffer can also be used for protein extraction from insect cells.

Bacterial Protein Extraction Buffer

20020 BPS Bioscience 500 ml
EUR 245

Description: BPS Bacterial Protein extraction Buffer enables extraction of both soluble and insoluble (inclusion bodies) proteins from bacteria. The buffer provides high efficiency recovery of over-expressed proteins and is fully compatible with HIS/GST-tag purification procedures and standard protein concentration determination assays. The buffer can also be used for protein extraction from insect cells.

ATCC25922 Standard bacterial Strains

S0077 Lifescience Market 100 ul
EUR 438

ATCC25923 Standard bacterial Strains

S0078 Lifescience Market 100 ul
EUR 438

ATCC17802 Standard bacterial Strains

S0079 Lifescience Market 100 ul
EUR 438

ATCC16404 Standard bacterial Strains

S0080 Lifescience Market 100 ul
EUR 438

ATCC13813 Standard bacterial Strains

S0092 Lifescience Market 100 ul
EUR 438

ATCC27853 Standard bacterial Strains

S0110 Lifescience Market 100 ul
EUR 438

ATCC10792 Standard bacterial Strains

S0111 Lifescience Market 100 ul
EUR 438

ATCC19606 Standard bacterial Strains

S0113 Lifescience Market 100 ul
EUR 438

Ricketssia like bacterial RT PCR kit

RTq-V283-100D Bioingentech 100T
EUR 860.4

Ricketssia like bacterial RT PCR kit

RTq-V283-150D Bioingentech 150T
EUR 969.6

Ricketssia like bacterial RT PCR kit

RTq-V283-50D Bioingentech 50T
EUR 717.6

Suez Filter Cartridge Bacterial 0.1um

WAT2274 Scientific Laboratory Supplies EACH
EUR 960

Human bacterial vaginosis,BV ELISA Kit

201-12-1087 SunredBio 96 tests
EUR 528

Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Bacterial vaginosis (BV) ELISA Kit

abx052798-96tests Abbexa 96 tests
EUR 801.6

Human bacterial vaginosis(BV)ELISA Kit

GA-E1103HM-48T GenAsia Biotech 48T
EUR 346.8

Human bacterial vaginosis(BV)ELISA Kit

GA-E1103HM-96T GenAsia Biotech 96T
EUR 559.2

Human bacterial vaginosis(BV)ELISA Kit

QY-E00922 Qayee Biotechnology 96T
EUR 433.2

Human bacterial vaginosis,BV ELISA Kit

YLA2512HU-48T Shanghai YL Biotech 48T
EUR 435

Human bacterial vaginosis,BV ELISA Kit

YLA2512HU-96T Shanghai YL Biotech 96T
EUR 562.5

Bacterial Counting Colorimetric Assay Kit

K511-2500 Biovision each
EUR 744

Bacterial Counting Colorimetric Assay Kit

K511-500 Biovision each
EUR 418.8

Sushi Peptide S3, bacterial recombinant

7611-100 Biovision each
EUR 444

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Intestine microbial composition adjustments in bladder most cancers sufferers: A case-control research in Harbin, China

Background and goals: This research aimed to discover the adjustments of intestine micro organism in bladder most cancers sufferers.

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

Strategies and research design: Newly recognized bladder most cancers sufferers had been recruited. All members accomplished a questionnaire about private conduct and weight loss program. Pyrosequencing of the overall genomic DNA extracted from human feces was carried out by Illumina HiSeq 2000. The copy variety of goal DNA for micro organism was decided by real-time quantitative PCR assay. Fecal brief chain fatty acids contents had been measured by gasoline chromatography (GC) evaluation. The concentrations of lipopolysaccharide and D-lactic acid in serum had been decided by enzyme-linked immunosorbent assay kits.

Outcomes: Fruit consumption was considerably decrease than in wholesome controls. The numbers of Clostridium cluster XI and Prevotella in bladder most cancers sufferers decreased. The numbers of area micro organism and Prevotella had been considerably and positively related to fruit consumption (r=0.002, p<0.05 for area micro organism; r=0.004, p<0.05 for Prevotella). The focus of butyric acid decreased considerably in bladder most cancers sufferers, and the portions of fecal butyric acid had been considerably and positively related to fruit consumption (r=0.610, p<0.01). The concentrations of lipopolysaccharide and D-lactic acid, two delicate markers of intestine permeability, had been larger in bladder most cancers sufferers.

Conclusions: Dysbiosis of intestine microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity had been present in bladder most cancers sufferers, which may be related to insufficient fruit consumption.

Oxalate Decarboxylase, Active Bacterial Recombinant

Background: Shotgun metagenomic sequencing has improved our understanding of the human intestine microbiota. Numerous DNA extraction strategies have been in comparison with discover protocols that robustly and most precisely mirror the unique microbial neighborhood buildings.

Nevertheless, these suggestions will be additional refined by contemplating the time and value calls for in coping with samples from very massive human cohorts. Moreover, fungal DNA extraction efficiency has thus far been little investigated.

Outcomes: We in contrast 6 DNA extraction protocols, MagPure Quick StoolDNA KF Equipment B, Macherey Nagel™ NucleoSpin™®Soil equipment, Zymo Analysis Fast-DNA™ Fecal/SoilMicrobe equipment, MOBIO DNeasy PowerSoil equipment, the guide non-commercial protocol MetaHIT, and the just lately printed protocol Q utilizing 1 microbial mock neighborhood (MMC) (containing eight bacterial and a couple of fungal strains) and fecal samples.

EZLys? Bacterial Protein Extraction Reagent

All samples had been manually extracted and subjected to shotgun metagenomics sequencing. Extracting DNA revealed excessive reproducibility inside all 6 protocols, however microbial extraction efficiencies various. The MMC outcomes demonstrated that bead dimension was a figuring out issue for fungal and bacterial DNA yields. In human fecal samples, the MagPure bacterial extraction carried out in addition to the standardized protocol Q however was quicker and less expensive.

Extraction utilizing the PowerSoil protocol resulted in a considerably greater ratio of gram-negative to gram-positive micro organism than different protocols, which could contribute to reported intestine microbial variations between wholesome adults.

Conclusions: We emphasize the significance of bead dimension choice for bacterial and fungal DNA extraction.

Extra importantly, the efficiency of the novel protocol MP matched that of the really helpful standardized protocol Q however consumed much less time, was less expensive, and is really helpful for additional large-scale human intestine metagenomic research.

Key phrases: DNA extraction; intestine microbiota; human fecal pattern; shotgun metagenomic sequencing.

Blood Genomic DNA Kit (No RNase A)

20-abx098864 Abbexa

EUR 460.80

EUR 276.00

200 rxns

50 rxns

Rapid Blood Genomic DNA Extraction Kit

BT4782 Bio Basic 50Preps
EUR 91.97

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-50rxns Abbexa 50 rxns
EUR 360

Blood Genomic DNA Extraction Mini Kit (100prep)

FABGK-100 Favorgen 100 preps
EUR 198

Blood Genomic DNA Extraction Mini Kit (300prep)

FABGK-300 Favorgen 300 preps
EUR 319.2

Blood genomic DNA extraction and purification kit

K1443-100 Biovision each
EUR 574.8

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92031 Bio Basic 2Plates, 192prep
EUR 210.85

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92032 Bio Basic 5Plates, 480prep
EUR 389.9

LumiPure genomic DNA Blood and Buccal Kit, 50

21553 Lumiprobe 50 preps
EUR 116.4

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit

SK8253 Bio Basic 50Preps
EUR 100.08

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit

SK8254 Bio Basic 100preps
EUR 144.86

Blood Genomic DNA Extraction MIni Kit (Automatic Machine) Sample

FABGK-AUTO-024 Favorgen 24 preps
EUR 180

Genomic DNA Kit

20-abx098076 Abbexa

EUR 526.80

EUR 292.80

200 rxns

50 rxns

96 Well Human Adult Normal Genomic DNA Plate - Peripheral Blood Leukocyte

D8234148-1 Biochain 1 plate
EUR 254

Soil Genomic DNA Kit

K1411-250 Biovision each
EUR 673.2

Soil Genomic DNA Kit

K1411-50 Biovision each
EUR 379.2

Plant Genomic DNA Kit

20-abx098077 Abbexa

EUR 493.20

EUR 276.00

200 rxns

50 rxns

Micro Genomic DNA Kit

20-abx098242 Abbexa

EUR 126.00

EUR 393.60

2 rxns

50 rxns

Plant Genomic DNA Kit

abx294004-50preps Abbexa 50 preps
EUR 477.6

Yeast Genomic DNA Kit

K1414-250 Biovision each
EUR 673.2

Yeast Genomic DNA Kit

K1414-50 Biovision each
EUR 379.2

Animal Genomic DNA Kit

abx294005-100preps Abbexa 100 preps
EUR 627.6

Animal Genomic DNA Kit

abx294005-50preps Abbexa 50 preps
EUR 477.6

Insect Genomic DNA Kit

K1412-250 Biovision each
EUR 673.2

Insect Genomic DNA Kit

K1412-50 Biovision each
EUR 379.2

Fungal Genomic DNA Kit

K1415-250 Biovision each
EUR 673.2

Fungal Genomic DNA Kit

K1415-50 Biovision each
EUR 379.2

Mollusc Genomic DNA Kit

K1413-250 Biovision each
EUR 673.2

Mollusc Genomic DNA Kit

K1413-50 Biovision each
EUR 379.2

Bacteria Genomic DNA Kit

20-abx098080 Abbexa

EUR 126.00

EUR 292.80

2 rxns

50 rxns

Genomic DNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, from a single donor

D1234148 Biochain 100 ug
EUR 286

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Bacterial DNA Extraction Kit (Proteinase K included)
BA50	GeneOn	50 preps	EUR 120

AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria
Z1322006	Biochain	1 kit (48 extractions) Including all required plastic disposables	EUR 216

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS423	Bio Basic	50Preps	EUR 98.36

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS624	Bio Basic	250preps	EUR 231.86

Bacterial Flagellin
VAdv-Ly0027	Creative Biolabs	50 µg	EUR 9391.2
Description: Bacterial flagellin, a TLR5 agonist vaccine adjuvant.

Agar Bacterial Tech 2Kg
281210	Scientific Laboratory Supplies	EACH	EUR 686.4

Collagenase (bacterial) antibody
20C-CR6033RP	Fitzgerald	500 ul	EUR 516
Description: Rabbit polyclonal Collagenase (bacterial) antibody

Bacterial Collagenase Assay Kit
3014	Chondrex	1 kit	EUR 700.32
Description: Bacterial Collagenase Assay Kit

Ricketssia like bacterial PCR kit
PCR-V283-48D	Bioingentech	50T	EUR 543.6

Ricketssia like bacterial PCR kit
PCR-V283-96D	Bioingentech	100T	EUR 686.4

Bacterial Protein Extraction Buffer
20010	BPS Bioscience	200 ml	EUR 130
Description: BPS Bacterial Protein extraction Buffer enables extraction of both soluble and insoluble (inclusion bodies) proteins from bacteria. The buffer provides high efficiency recovery of over-expressed proteins and is fully compatible with HIS/GST-tag purification procedures and standard protein concentration determination assays. The buffer can also be used for protein extraction from insect cells.

Bacterial Protein Extraction Buffer
20020	BPS Bioscience	500 ml	EUR 245
Description: BPS Bacterial Protein extraction Buffer enables extraction of both soluble and insoluble (inclusion bodies) proteins from bacteria. The buffer provides high efficiency recovery of over-expressed proteins and is fully compatible with HIS/GST-tag purification procedures and standard protein concentration determination assays. The buffer can also be used for protein extraction from insect cells.

ATCC25922 Standard bacterial Strains
S0077	Lifescience Market	100 ul	EUR 438

ATCC25923 Standard bacterial Strains
S0078	Lifescience Market	100 ul	EUR 438

ATCC17802 Standard bacterial Strains
S0079	Lifescience Market	100 ul	EUR 438

ATCC16404 Standard bacterial Strains
S0080	Lifescience Market	100 ul	EUR 438

ATCC13813 Standard bacterial Strains
S0092	Lifescience Market	100 ul	EUR 438

ATCC27853 Standard bacterial Strains
S0110	Lifescience Market	100 ul	EUR 438

ATCC10792 Standard bacterial Strains
S0111	Lifescience Market	100 ul	EUR 438

ATCC19606 Standard bacterial Strains
S0113	Lifescience Market	100 ul	EUR 438

Ricketssia like bacterial RT PCR kit
RTq-V283-100D	Bioingentech	100T	EUR 860.4

Ricketssia like bacterial RT PCR kit
RTq-V283-150D	Bioingentech	150T	EUR 969.6

Ricketssia like bacterial RT PCR kit
RTq-V283-50D	Bioingentech	50T	EUR 717.6

Suez Filter Cartridge Bacterial 0.1um
WAT2274	Scientific Laboratory Supplies	EACH	EUR 960

Human bacterial vaginosis,BV ELISA Kit
201-12-1087	SunredBio	96 tests	EUR 528

Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Bacterial vaginosis (BV) ELISA Kit
abx052798-96tests	Abbexa	96 tests	EUR 801.6

Human bacterial vaginosis(BV)ELISA Kit
GA-E1103HM-48T	GenAsia Biotech	48T	EUR 346.8

Human bacterial vaginosis(BV)ELISA Kit
GA-E1103HM-96T	GenAsia Biotech	96T	EUR 559.2

Human bacterial vaginosis(BV)ELISA Kit
QY-E00922	Qayee Biotechnology	96T	EUR 433.2

Human bacterial vaginosis,BV ELISA Kit
YLA2512HU-48T	Shanghai YL Biotech	48T	EUR 435