Genetici-Technologies

by Charles Jul 23, 2020 Assay Kits

Best Column Bacterial Genomic DNA Miniprep Kit

Metagenomic water high quality monitoring with a conveyable laboratory

We describe the technical feasibility of metagenomic water high quality evaluation utilizing solely moveable tools, for instance mini-vacuum pumps and filtration models, mini-centrifuges, mini-PCR machines and the memory-stick sized MinION of Oxford Nanopore Applied sciences, for the library preparation and sequencing of 16S rRNA gene amplicons.

1 KB DNA LADDER, 500UL PER KIT

Utilizing this moveable toolbox on website, we efficiently characterised the microbiome of water samples collected from Birtley Sewage Therapy Plant, UK, and its environs. We additionally demonstrated the applicability of the moveable metagenomics toolbox in a low-income nation by surveying water samples from the Akaki River round Addis Ababa, Ethiopia.

The 16S rRNA gene sequencing workflow, together with DNA extraction, PCR amplification, sequencing library preparation, and sequencing was completed inside one working day. The metagenomic knowledge grew to become obtainable inside 24-72 h, relying on web pace.

Bacterial DNA Extraction Kit (Proteinase K included)

Metagenomic evaluation clearly distinguished the microbiome of pristine samples from sewage influenced water samples. Metagenomic evaluation recognized the potential function of two bacterial genera not conventionally monitored, Arcobacter and Aeromonas, as predominant faecal air pollution indicators/waterborne hazards.

 

Genetici-Technologies
Genetici-Technologies

Subsequent quantitative PCR evaluation validated the excessive Arcobacter butzleri abundances noticed within the city influenced Akaki River water samples by moveable subsequent technology sequencing with the MinION system. General, our discipline deployable metagenomics toolbox advances the aptitude of scientists to comprehensively monitor microbiomes wherever on the planet, together with within the water, meals and drinks industries, the well being providers, agriculture and past.

Key phrases: Fecal supply monitoring; Area kits; Metagenomics; MinION nanopore sequencing; Water high quality; Waterborne hazards.

Bacterial DNA Purification Kit II
K1457-50 50 Preps
EUR 381.6

EcoSpin Bacterial Genomic DNA Kit
E1050 50 rxn
EUR 53.9
Description: EcoSpin Bacterial Genomic DNA Kit is designed as a simple and convenient purification of high quality genomic DNA from Gram (-) negative and Gram (+) positive bacterial cells. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 25 minutes and purified DNA can be used directly in PCR, qPCR, sequencing and enzymatic reactions.

Milk Bacterial DNA Extraction Kit (50prep)
FAMBD-001 50 preps
EUR 328.8

Milk Bacterial DNA Extraction Kit (50prep)
FAMBD_001 1 kit
EUR 134.5

Bacterial Genomic DNA Isolation Kit
K309-100 each
EUR 451.2

GeneMATRIX TISSUE & BACTERIAL DNA Purification Kit
E3551-01 50preps
EUR 98.1
Description: Kit for isolation of total DNA from human and animal tissues and bacteria

GeneMATRIX TISSUE & BACTERIAL DNA Purification Kit
E3551-02 150preps
EUR 275.77
Description: Kit for isolation of total DNA from human and animal tissues and bacteria

AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria
Z1322006 1 kit (48 extractions) Including all required plastic disposables
EUR 259

Bacterial DNA Extraction Kit (Proteinase K included)
BA50 50 preps
EUR 120

Bacterial DNA Extraction Kit (Proteinase K included)
BA05 5 preps Ask for price

Bacterial DNA Extraction Kit (Proteinase K included)
BA100 100 preps
EUR 165.6

Bacterial DNA Extraction Kit (Proteinase K included)
BA96-05 5x96 preps
EUR 694

HiPer® Bacterial Genomic DNA Extraction
HTBM008E-10PR 1 unit
EUR 32.63
Description: HiPer® Bacterial Genomic DNA Extraction

HiPer® Bacterial Genomic DNA Extraction
HTBM009E-10PR 1 unit
EUR 37.4
Description: HiPer® Bacterial Genomic DNA Extraction

Insta NX® Bacterial Genomic DNA Purifica
MBIN006-12PR 1 unit
EUR 71.73
Description: Insta NX® Bacterial Genomic DNA Purifica

SaMag Bacterial DNA Extraction kit For use with SaMag-12/24 instruments; extraction of bacterial DNA of from clinical specimens or coltures
SM006 48
EUR 187.48

HiPurA® Bacterial Genomic DNA Miniprep P
MB505-20PR 1 unit
EUR 38.72
Description: HiPurA® Bacterial Genomic DNA Miniprep P

HiPurA® Bacterial Genomic DNA Miniprep P
MB505-250PR 1 unit
EUR 415.6
Description: HiPurA® Bacterial Genomic DNA Miniprep P

HiPurA® Bacterial Genomic DNA Miniprep P
MB505-50PR 1 unit
EUR 91.51
Description: HiPurA® Bacterial Genomic DNA Miniprep P

Column-Pure Bacterial Genomic DNA Isolation Kit
D511 50 Preparations
EUR 195

HiPurA® 96-Bacterial Genomic DNA Purific
MB548-1X96PR 1 unit
EUR 172.52
Description: HiPurA® 96-Bacterial Genomic DNA Purific

GeneMATRIX BACTERIAL & YEAST GENOMIC DNA Purification Kit
E3580-01 50preps
EUR 95.92

GeneMATRIX BACTERIAL & YEAST GENOMIC DNA Purification Kit
E3580-02 150preps
EUR 270.32

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS423 50Preps
EUR 98.36

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS624 250preps
EUR 231.86

ExoQuali™ Bacterial MVs Isolation Kit (bacterial culture media)
CEIK-092L-10T 10 T Ask for price

ExoQuali™ Bacterial MVs Isolation Kit (bacterial culture media)
CEIK-092L-20T 20 T Ask for price

GF-1 Bacterial Starter Kit / Taq DNA Polymerase, 25 preps
GF-BA-K each Ask for price

GF-1 Bacterial DNA Extraction Kit (Proteinase K included), 50 preps
GF-BA-050 each Ask for price

GF-1 Bacterial DNA Extraction Kit (Proteinase K included), 100 preps
GF-BA-100 each Ask for price

Bacterial Flagellin
VAdv-Ly0027 50 µg
EUR 9391.2
Description: Bacterial flagellin, a TLR5 agonist vaccine adjuvant.

GF-1 Bacterial Starter Kit / Chromo Taq DNA Polymerase, 25 preps
GF-BA-KW each Ask for price

Bacterial Transformation Kit
MBT127-10R 1 unit
EUR 18.24
Description: Bacterial Transformation Kit

Bacterial Transformation Kit
MBT127-20R 1 unit
EUR 32.87
Description: Bacterial Transformation Kit

Bacterial Transformation Kit
MBT127-40R 1 unit
EUR 58.64
Description: Bacterial Transformation Kit

EasyRNA? Bacterial RNA Kit
K1351-250 each
EUR 1161.6

EasyRNA? Bacterial RNA Kit
K1351-50 each
EUR 444

Bacterial Recombinant MDH
6357-100 each
EUR 574.8

Bacterial Recombinant GOR
6380-100 each
EUR 548.4

Ricketssia like bacterial
Oneq-V283-OneqV283100D Oneq-V283-100D
EUR 515

Ricketssia like bacterial
Oneq-V283-OneqV283150D Oneq-V283-150D
EUR 594

 

Intestine microbial composition adjustments in bladder most cancers sufferers: A case-control research in Harbin, China

Background and goals: This research aimed to discover the adjustments of intestine micro organism in bladder most cancers sufferers.

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

Strategies and research design: Newly recognized bladder most cancers sufferers had been recruited. All members accomplished a questionnaire about private conduct and weight loss program. Pyrosequencing of the overall genomic DNA extracted from human feces was carried out by Illumina HiSeq 2000. The copy variety of goal DNA for micro organism was decided by real-time quantitative PCR assay. Fecal brief chain fatty acids contents had been measured by gasoline chromatography (GC) evaluation. The concentrations of lipopolysaccharide and D-lactic acid in serum had been decided by enzyme-linked immunosorbent assay kits.

Outcomes: Fruit consumption was considerably decrease than in wholesome controls. The numbers of Clostridium cluster XI and Prevotella in bladder most cancers sufferers decreased. The numbers of area micro organism and Prevotella had been considerably and positively related to fruit consumption (r=0.002, p<0.05 for area micro organism; r=0.004, p<0.05 for Prevotella). The focus of butyric acid decreased considerably in bladder most cancers sufferers, and the portions of fecal butyric acid had been considerably and positively related to fruit consumption (r=0.610, p<0.01). The concentrations of lipopolysaccharide and D-lactic acid, two delicate markers of intestine permeability, had been larger in bladder most cancers sufferers.

Conclusions: Dysbiosis of intestine microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity had been present in bladder most cancers sufferers, which may be related to insufficient fruit consumption.

 

Oxalate Decarboxylase, Active Bacterial Recombinant

Background: Shotgun metagenomic sequencing has improved our understanding of the human intestine microbiota. Numerous DNA extraction strategies have been in comparison with discover protocols that robustly and most precisely mirror the unique microbial neighborhood buildings.

Nevertheless, these suggestions will be additional refined by contemplating the time and value calls for in coping with samples from very massive human cohorts. Moreover, fungal DNA extraction efficiency has thus far been little investigated.

Outcomes: We in contrast 6 DNA extraction protocols, MagPure Quick StoolDNA KF Equipment B, Macherey Nagel™ NucleoSpin™®Soil equipment, Zymo Analysis Fast-DNA™ Fecal/SoilMicrobe equipment, MOBIO DNeasy PowerSoil equipment, the guide non-commercial protocol MetaHIT, and the just lately printed protocol Q utilizing 1 microbial mock neighborhood (MMC) (containing eight bacterial and a couple of fungal strains) and fecal samples.

EZLys? Bacterial Protein Extraction Reagent
All samples had been manually extracted and subjected to shotgun metagenomics sequencing. Extracting DNA revealed excessive reproducibility inside all 6 protocols, however microbial extraction efficiencies various. The MMC outcomes demonstrated that bead dimension was a figuring out issue for fungal and bacterial DNA yields. In human fecal samples, the MagPure bacterial extraction carried out in addition to the standardized protocol Q however was quicker and less expensive.

Extraction utilizing the PowerSoil protocol resulted in a considerably greater ratio of gram-negative to gram-positive micro organism than different protocols, which could contribute to reported intestine microbial variations between wholesome adults.

Conclusions: We emphasize the significance of bead dimension choice for bacterial and fungal DNA extraction.

Extra importantly, the efficiency of the novel protocol MP matched that of the really helpful standardized protocol Q however consumed much less time, was less expensive, and is really helpful for additional large-scale human intestine metagenomic research.

Key phrases: DNA extraction; intestine microbiota; human fecal pattern; shotgun metagenomic sequencing.

EcoSpin Blood Genomic DNA Kit
EcoBGD-50x 50 rxn
EUR 53.9
Description: EcoSpin Blood Genomic DNA Kit is is designed as a simple and convenient purification of high quality genomic DNA from whole blood. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard protocol lasts less than 25 minutes and purified DNA can be used directly in PCR, qPCR, Southern blotting and enzymatic reactions.

Pig Blood Genomic DNA
PG-705 0.1mg
EUR 223

Blood Genomic DNA Midi
GGD2001 20 preps
EUR 203.16

Blood Genomic DNA Midi
GGD2002 50 preps
EUR 457.11

Blood Genomic DNA Maxi
GGM2001 10 preps
EUR 203.16

Blood Genomic DNA Maxi
GGM2002 25 preps
EUR 457.11

Human Blood Genomic DNA
HG-705 0.05mg
EUR 319

Bovine Blood Genomic DNA
BG-705 0.1mg
EUR 223

Blood Genomic DNA Kit (5-250 ul)
20-abx098078
  • Ask for price
  • Ask for price
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (5-250 µl)
abx098078-100l 100 µl
EUR 287.5

Blood Genomic DNA Kit (5-250 µl)
abx098078-1ml 1 ml Ask for price

Blood Genomic DNA Kit (5-250 µl)
abx098078-200l 200 µl
EUR 512.5

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-For200mlBlood For 200 ml Blood
EUR 427.2

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-For50mlBlood For 50 ml Blood
EUR 260.4

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-1ml 1 ml Ask for price

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-200l 200 µl
EUR 437.5

Blood Genomic DNA Kit (0.1-20 ml)
abx098074-100l 100 µl
EUR 275

Column-Pure Blood Genomic DNA Kit
D203-Mini-1 50 Preparations
EUR 105

Blood Genomic DNA Kit (No RNase A)
20-abx098864
  • Ask for price
  • Ask for price
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (No RNase A)
abx098864-100l 100 µl
EUR 287.5

Blood Genomic DNA Kit (No RNase A)
abx098864-1ml 1 ml Ask for price

Blood Genomic DNA Kit (No RNase A)
abx098864-200l 200 µl
EUR 475

Rapid Blood Genomic DNA Extraction Kit
BT4782 50Preps
EUR 91.97

Blood Genomic DNA Mini Kit (0.1-1ml)
MBS278035-50Preps 50Preps
EUR 215

Magnetic bead Blood/Cell Genomic DNA Kit
PDM06-0100 100 rcs
EUR 265

Blood Genomic DNA Kit (with Magnetic Stand)
abx098867-50rxns 50 rxns
EUR 360

Blood Genomic DNA Kit (with Magnetic Stand)
abx098867-100l 100 µl
EUR 375

Blood Genomic DNA Kit (with Magnetic Stand)
abx098867-1ml 1 ml Ask for price

Blood Genomic DNA Kit (with Magnetic Stand)
abx098867-200l 200 µl Ask for price

Magnetic Beads-Based Blood Genomic DNA Kit
abx460020-1096tests 10 × 96 tests Ask for price

Magnetic Beads-Based Blood Genomic DNA Kit
abx460020-596tests 5 × 96 tests Ask for price

 

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