Combined use of single molecule real-time DNA sequencing technology and culture-dependent methods to analyze the functional microorganisms in inoculated raw wheat Qu.

Dec 10, 2020 Assay Kits

Combined use of single molecule real-time DNA sequencing technology and culture-dependent methods to analyze the functional microorganisms in inoculated raw wheat Qu.

Inoculated uncooked wheat Qu (IRWQ), which could be produced with excessive effectivity and low value whereas sustaining secure high quality, is a brand new Qu that has been utilized to Huangjiu brewing. On this research, single molecule real-time DNA sequencing know-how and culture-dependent strategies have been mixed for the primary time to check the microbiota and the perform of the precept microorganisms in IRWQ. The glucoamylase, amylase and protease contents of IRWQ have been 1.17, 1.55 and a pair of.87-times better, respectively, than these of conventional wheat Qu. Like conventional wheat Qu, the principle risky taste compounds in IRWQ have been alcohols; nonetheless, the esters content material was a lot increased and the acids content material was a lot decrease. Single molecule real-time DNA sequencing know-how recognized 18 fungal species and 59 bacterial species in IRWQ.

Then, 30 species have been remoted by culture-dependent strategies. These species represented about 80% of the entire fungal microbiota and 35% of the entire micro organism microbiota. Molds have been the principle contributors of glucoamylase, amylase and protease actions. Aspergillus flavus had the very best glucoamylase and protease exercise. Bacillus subtilis and Bacillus amyloliquefaciens additionally produced excessive hydrolytic enzyme actions. Saccharomyces cerevisiae produced the biggest quantities of aromatics compounds, alcohols, esters and acids. Aldehydes and ketones are primarily produced by molds. With the speedy improvement of molecular organic strategies, DNA microarray has proven nice benefit in biology and medication because it permits high-throughput measurement of assorted organic parameters.

Trypanosomiasis stays the main focus amongst quite a few human blood parasitic illnesses. The DNA microarray is a helpful approach for finding out the trypanosome genome and parasite-host interplay, in addition to for vaccine screening and drug improvement. This assessment offers an replace on the appliance of DNA microarray in human analysis on Trypanosoma brucei and Trypanosoma cruzi.

Excessive-Throughput DNA Plasmid Transfection Utilizing Acoustic Droplet Ejection Know-how.

The Labcyte Echo acoustic liquid handler permits correct droplet ejection at excessive velocity from a supply effectively plate to a vacation spot plate. It has already been utilized in varied miniaturized organic assays, akin to quantitative PCR (q-PCR), quantitative real-time PCR (q-RT-PCR), protein crystallization, drug screening, cell shelling out, and siRNA transfection. Nonetheless, no plasmid DNA transfection assay has been revealed to date utilizing this shelling out know-how. On this research, we evaluated the power of the Echo 550 system to carry out plasmid DNA transfection in 384-well plates.
As a result of excessive throughput of this system, we concurrently optimized the three foremost parameters of a transfection course of: dilution of the transfection reagent, DNA quantity, and beginning DNA focus. We outlined a four-step protocol whose optimum settings allowed us to transfect HeLa cells with as much as 90% effectivity and attain a co-expression of almost 100% inside transfected cells in co-transfection experiments. This quick, dependable, and automatic protocol opens new methods to simply and quickly determine optimum transfection settings for a given cell sort.
Moreover, it permits straightforward software-based transfection management and multiplexing of plasmids distributed on wells of a supply plate. This new improvement might result in new array functions, akin to human ORFeome protein expression or CRISPR-Cas9-based gene perform validation in nonpooled screening methods.
Combined use of single molecule real-time DNA sequencing technology and culture-dependent methods to analyze the functional microorganisms in inoculated raw wheat Qu.
Combined use of single molecule real-time DNA sequencing technology and culture-dependent methods to analyze the functional microorganisms in inoculated raw wheat Qu.

Aberrant DNA Methylation of IGF2-H19 Locus in Human Fetus and in Spermatozoa From Assisted Reproductive Applied sciences.

Given the upper threat of growing imprinting issues in assisted reproductive know-how (ART)-conceived kids, we hypothesized that ART could have an effect on DNA methylation of the insulin-like progress issue 2 (IGF2), H19, small nuclear ribonucleoprotein polypeptide N (SNRPN) differentially methylated areas (DMRs) on the fetal stage, which in flip could also be related to sperm abnormalities. A complete of four affected person teams have been recruited, specifically, multifetal discount following in vitro fertilization (IVF)/ intracytoplasmic sperm injection (ICSI; n = 56), multifetal discount following managed ovarian hyperstimulation (COH; n = 42), male sufferers with regular semen parameters denoted as normozoospermia group (NZ) for IVF (n = 36), and male sufferers presenting with asthenozoospermia (OAZ) for ICSI (n = 38).
The expression ranges and the DNA methylation standing of IGF2- H19 and SNRPN DMRs within the fetuses and the semen samples have been evaluated by real-time quantitative polymerase chain response and pyrosequencing. In our outcomes, the expression ranges of H19 have been considerably increased, whereas the methylation charges have been decrease in IVF-conceived fetuses in comparison with the management group ( P < .05). Moreover, increased methylation charges of IGF2 DMR2 and SNRPN DMR have been detected each in IVF- and ICSI-conceived fetuses ( P < .05). The information additional indicated that the sufferers who introduced with nearly all of the CpG websites within the H19 DMR area that have been decrease methylated have been these within the OAZ group.

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1358.4

ApexPrep DNA Plasmid Miniprep Kit

A5001-300 300 tests
EUR 338.4

ApexPrep DNA Plasmid Miniprep Kit

A5001-50 50 tests
EUR 115.2

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Plasmid DNA Extraction Mini Kit (100prep)

FAPDE-001 100 preps
EUR 168

Plasmid DNA Extraction Mini Kit (300prep)

FAPDE-001-1 300 preps
EUR 241.2

Plasmid DNA Extraction Mini Kit (100prep)

FAPDE-100 100 preps
EUR 154.8

Plasmid DNA Extraction Mini Kit (300prep)

FAPDE-300 300 preps
EUR 206.4

Plasmid DNA extraction and purification kit

K1445-100
EUR 444

Column Packing Kit

PACK-KIT 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)

P402 - Ask for price

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

T4 DNA Ligase Plasmid

PVT7008 2 ug
EUR 614.4

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

96-Well Plate Plasmid DNA Miniprep Kit

BS415 5XPlates, 480prep
EUR 300.37

96-Well Plate Plasmid DNA Miniprep Kit

BS4152 2XPlates, 192prep
EUR 186.19

Plasmid DNA Extraction Mini Kit (300prep)-capless

FAPDE-300-CAPLESS 300 preps
EUR 206.4

Genorise® Plasmid DNA Extraction Kit 1000

GR101073 1000
EUR 1020

Genorise® Plasmid DNA Extraction Kit 100

GR101073-100 100
EUR 172.8

Genorise® Plasmid DNA Extraction Kit 200

GR101073-200 200
EUR 309.6

Genorise® Plasmid DNA Extraction Kit 50

GR101073-50 50
EUR 104.4

Genorise® Plasmid DNA Extraction Kit 500

GR101073-500 500
EUR 576

Plasmid DNA Extraction Kit (Rnase A included)

PL05 5 preps Ask for price

Plasmid DNA Extraction Kit (Rnase A included)

PL100 100 preps
EUR 156

Plasmid DNA Extraction Kit (Rnase A included)

PL200 200 preps
EUR 226.8

Plasmid DNA Extraction Kit (Rnase A included)

PL50 50 preps
EUR 112.8

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1358.4

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 966

Plasmid DNA Extraction and Purification

EP10014 100 Tests
EUR 403.2

Plasmid DNA Maxiprep (10 preps)

9K-006-0023 10Preps
EUR 257.32

Plasmid DNA Maxiprep (30 preps)

9K-006-0026 30preps
EUR 651.95

Relaxed plasmid DNA - 0.050 mg

RDNA-050UG 0.050 mg
EUR 181.67
Description: This product includes 0.050 mg of relaxed plasmid DNA at 1 mg/ml in TE buffer

Relaxed plasmid DNA - 1 mg

RDNA-1MG 1 mg
EUR 2298.23
Description: This product includes 1 mg of relaxed plasmid DNA at 1 mg/ml in TE buffer

Supercoiled plasmid DNA - 0.050 mg

SDNA-050UG 0.050 mg
EUR 160.91
Description: This product includes 0.050 mg of supercoiled plasmid DNA at 1 mg/ml in TE buffer

Supercoiled Plasmid DNA -1 mg

SDNA-1MG 1 mg
EUR 1752.66
Description: This product includes 1 mg of supercoiled plasmid DNA at 1 mg/ml concentration in TE buffer.

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1358.4

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 183.6

Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXMT-KIT 30ML
EUR 237.12

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 5929.2

Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXHM-KIT 30ML
EUR 245.1

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 5929.2

EZ-10 Spin Column Plasmid DNA Miniprep Kit

BS413 50Preps
EUR 91.97

EZ-10 Spin Column Plasmid DNA Miniprep Kit

BS414 100preps
EUR 114.82

Plasmid DNA Extraction Midi Kit (25prep), Ion Exchange

FAPDE-002 25 preps
EUR 259.2

Plasmid DNA Extraction Midi Kit (50prep), Ion Exchange

FAPDE-002-1 50 preps
EUR 380.4

Plasmid DNA Extraction Maxi Kit (10prep), Ion Exchange

FAPDE-003 10 preps
EUR 241.2

Plasmid DNA Extraction Maxi Kit (20prep), Ion Exchange

FAPDE-003-1 20 preps
EUR 337.2

Plasmid DNA ExtractionMaxi Kit (10prep), EndoToxinFree, Ion Exchange

FAPDE-003-EF 10 preps
EUR 259.2

Plasmid DNA Extraction Mini Kit (Automatic Machine) Sample

FAPDE-AUTO-024 24 preps
EUR 168

96-well Plasmid DNA Extraction Kit (1 plate)

FAPWE-96001 1 prep
EUR 175.2

96-well Plasmid DNA Extraction Kit (2 plates)

FAPWE-96002 2 preps
EUR 225.6

96-well Plasmid DNA Extration Kit (4 plates)

FAPWE-96004 4 preps
EUR 328.8

Miniprep Kit For Plasmid DNA Extraction And Purification

MB1005-100Reactions 100 Reactions
EUR 171.6

Miniprep Kit For Plasmid DNA Extraction And Purification

MB1005-250Reactions 250 Reactions
EUR 310.8

Miniprep Kit For Plasmid DNA Extraction And Purification

MB1005-50Reactions 50 Reactions
EUR 144

Genorise® Pure Plasmid DNA Mini Kit 50

GR101078 50
EUR 178.8

Genorise® Pure Plasmid DNA Mini Kit 100

GR101078-100 100
EUR 320.4

Genorise® Pure Plasmid DNA Mini Kit 200

GR101079 200
EUR 572.4

Genorise® Pure Plasmid DNA Mini Kit 500

GR101079-500 500
EUR 1288.8

EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid

BS4139 50Preps
EUR 105.67

EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid

BS4149 100preps
EUR 136.74

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2558.4

ApexPrep DNA Plasmid Miniprep Column Only

A5002-300 300 tests
EUR 296.4

ApexPrep DNA Plasmid Miniprep Column Only

A5002-50 50 tests
EUR 104.4

EZ-10 Spin Column Plasmid DNA Min-preps Kit, Low Copy Plasmid

BS6149 250preps
EUR 251.05

vWF Acty. Kit

ABP-ACT-KIT 12 x 8 microwells
EUR 513.6

vWF Ant. Kit

ABP-TOT-KIT 12 x 8 microwells
EUR 472.8

EZ-500 Spin Column Plasmid DNA Maxi-preps Kit

BS4654 4preps
EUR 150.92

EZ-500 Spin Column Plasmid DNA Maxi-preps Kit

BS466 20Preps
EUR 466.63

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit

BS467 10Preps
EUR 78.28

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit

BS468 50Preps
EUR 133.08

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit

BS469 250preps
EUR 364.32

EZ-10 Spin Column Plasmid DNA Min-preps Kit

BS614 250preps
EUR 199.9

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92028 2XPlates, 192prep
EUR 254.7

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92029 5XPlates, 480prep
EUR 437.4

Plasmid DNA Extraction Maxi Kit (4prep), FavorFilter, Ion Exchange

FAFTE-001 4 preps
EUR 180

Plasmid DNA Extraction Maxi Kit (10prep), FavorFilter, Ion Exchange

FAFTE-001-1 10 preps
EUR 249.6

Plasmid DNA Extraction Midi Kit (25prep), FavorFilter, Ion Exchange

FAFTE-002 25 preps
EUR 294

Plasmid DNA Extraction Midi Kit (50prep), FavorFilter, Ion Exchange

FAFTE-002-1 50 preps
EUR 441.6

Plasmid DNA Extraction Midi Kit (25prep), EndoToxinFree, Ion Exchange

FAPDE-002-EF 25 preps
EUR 276

Plasmid DNA Midi Endotoxin Removal kit(25prep)-spin column

FAPDE-005B-025 25 preps
EUR 198

Plasmid DNA Midi Endotoxin Removal kit(50prep)-spin column

FAPDE-005B-050 50 preps
EUR 272.4

Plasmid DNA Maxi Endotoxin Removal kit(10prep)-spin column

FAPDE-005C-010 10 preps
EUR 186

Plasmid DNA Maxi Endotoxin Removal kit(20prep)-spin column

FAPDE-005C-020 20 preps
EUR 249.6

PrecisionX Multiplex gRNA Cloning Kit

CAS9-GRNA-KIT 10 rxn
EUR 534

hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)

CAS620A-KIT 1 kit
EUR 2582.4
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN300A-KIT 1 Kit
EUR 3357.6

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN400A-KIT 1 Kit
EUR 3357.6

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN410A-KIT 1 Kit
EUR 5202

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN412A-KIT 1 Kit
EUR 5202

Filtration System 47mm Complete Assembly

5809/KIT EACH
EUR 427.69

mRNAExpress mRNA Synthesis kit (5 reactions)

MR-KIT-1 5 reactions
EUR 1382.4

42 Component Terpene Kit in Methanol

CAN-TERP-KIT 1ML
EUR 287.28

DNA Assay Kit

6023 1 kit
EUR 216.6
Description: DNA Assay Kit

Circulating DNA Kit

abx098870-50rxns 50 rxns
EUR 477.6

Genomic DNA Kit

20-abx098076
  • EUR 526.80
  • EUR 292.80
  • 200 rxns
  • 50 rxns

Circulating DNA Kit

abx298027-50rxns 50 rxns
EUR 477.6

DNA Pure Kit

FYG601-100P 100 Preps Ask for price

DNA Pure Kit

FYG601-300P 300 Preps Ask for price

Mycoplasma DNA Kit

K1416-250
EUR 673.2

Mycoplasma DNA Kit

K1416-50
EUR 379.2

DNA Concentrator Kit 

P-1006-1 50 Samples
EUR 90.2

DNA Concentrator Kit 

P-1006-2 100 Samples
EUR 171.6

DNA Assay Kit

Z5030023 250 assays
EUR 776.4
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

DNA Concentrator Kit 

P-1006
  • EUR 303.60
  • EUR 90.20
  • EUR 171.60
  • 100 Samples
  • 50 Samples
  • 100 Samples

Genorise® Feline EGF Expression Plasmid DNA

GR233001 10 µg
EUR 238.8

Genorise® Feline TNFA Expression Plasmid DNA

GR233004 10 µg
EUR 238.8

Canadian Pesticide Kit Containing All 6 Mixes

CAN-CAN-KIT 1ML
EUR 758.1

AxioCam Erc5s rev 2, w/Accessory Kit

ZV-Erc5sAC-Kit 1 KIT
EUR 1611
Description: AxioCam Erc5s rev 2, w/Accessory Kit- connection cable Y-USB 2.0.- HDMI cable to DVI-D- SD card- IR remote control

EZ-10 Spin Column Endotoxin-Free Plasmid DNA Miniprep Kit

BS71918 50Preps
EUR 149.52

Plasmid DNA Extraction Maxi Kit (10prep), FavorFilter, EndotoxinFree, Ion Exchange

FAFTE-001-1-EF 10 preps
EUR 259.2

Plasmid DNA Extraction Maxi Kit (4prep), FavorFilter, EndotoxinFree, Ion Exchange

FAFTE-001-EF 4 preps
EUR 186

96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based)

B814152-0002 2XPlates, 2XPlates
EUR 186.19

96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based)

B814152-0005 5XPlates, 5XPlates
EUR 300.37

High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA)

20-abx098008
  • EUR 260.40
  • EUR 427.20
  • 1 ml
  • 5 ml

Mouse Anti-DNA Antibody (DNA) ELISA Kit

abx257816-96tests 96 tests
EUR 895.2

EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit

SK1249 4Maxi-Preps, 4prep
EUR 182.24

EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit

SK1250 20Maxi-Preps, 20prep
EUR 614.88

Genorise® Feline IL-10 Expression Plasmid DNA

GR233002 10 µg
EUR 238.8

Genorise® Feline IL-1B Expression Plasmid DNA

GR233003 10 µg
EUR 238.8

DNA Damage Quantification Kit

55R-1345 25 assays
EUR 1012.8
Description: Assay Kit for Quantification of DNA Damage in the research laboratory

Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 990
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 462
Description: Genomic DNA Isolation Kit for use in the research laboratory

Blood Genomic DNA Kit

abx098868-50rxns 50 rxns
EUR 326.4
The outcomes demonstrated that the epigenetic dysregulations of IGF2-H19 and SNRPN DMRs that have been brought on by ART have been famous within the fetuses. Furthermore, the current research recommended that epigenetic perturbations of the H19 DMR could be a key biomarker for spermatogenesis defects in people.

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